抗冻肽基因在热激表达盒式载体中的构建

克隆于细菌表达载体ｐ＾ＧＥＭ－３ｘｆ（－）中的ＡＦＰ基因经ＳａｌＩ和ＫｐｎＩＩ消化后,插入到热激盒式表达载体ｐ＾ＭＡ４１２的ＳａｌＩ和ＫｐｎＩ位点中,连接于可融合的报道基因元。     

升麻总皂苷在体肠吸收动力学研究

考察了升麻提取物中升麻苷H-2、升麻苷H-1、27-脱氧升麻亭、升麻醇-3-O-β-D吡喃木糖苷、25-O-乙酰升麻醇木糖苷5种主要有效成分在大鼠的肠吸收性质.以酚红为标示物,运用大鼠在体单向肠灌流模型并采用HPLC/MS/MS法测定灌流液中5种主要有效成分的浓度变化.结果发现,升麻苷H-2、升麻苷H-1、27-脱氧升...     

A new semisynthetic ergot peptide alkaloid: DCN 203-922

Summary We report the synthesis, stereochemistry and preliminary pharmacological evaluation of DCN 203-922, a novel ergot alkaloid of the cyclol type, which contains in its peptide moiety the uncommon amino acid L-allo-isoleucine.Part of this paper was reported by this author at the Herbstversammlung der Schweizerischen Chemischen Gesellschaft, Bern, in October 1986.     

Increased responsiveness of the hypothalamic-pituitary axis to steroid feedback effects in ovariectomized rats treated neonatally with monosodium L-glutamate

Summary Chronic ovariectomized rats treated neonatally with MSG showed reduced circulating concentrations of LH coupled with elevated hypothalamic LHRH stores. Despite the apparent loss of LHRH secretion, the small pituitary glands showed an increased density of LHRH receptors and normal responsiveness to the releasing hormone. The positive feedback effects of progesterone on LH release in oestrogen-primed animals was greatly exaggerated reflecting the build-up of hypothalamic LHRH stores without loss of pituitary responsiveness to LHRH.     

In vitro biosynthesis of juvenile hormone III by the corpora allata ofCalliphora vomitoria and its role in ovarian maturation and sexual receptivity

Summary JH III is the only JH detected by GLC-MS in medium from in vitro incubations of corpora allata of adult females ofCalliphora vomitoria. When corpora allata were removed from females at various times during the reproductive cycle and the JH III produced by the glands in vitro measured by a JH III radioimmunoassay, an increase in the level of synthesis was found to occur before previtellogenesis (0–24 h). A second increase appeared at the onset of vitellogenesis (72–83 h) and continued until the end of vitellogenesis (96 h) and the occurrence of chorionation (120 h). Since sexual receptivity develops with vitellogenesis, the significantly higher levels of JH III biosynthesis in vitro at this time supports a possible role for JH in the acquisitive of receptivity.     

Laminin五肽对胃癌细胞系MGc－803的体外抑制作用

描述了Ｌａｍｉｎｉｎ五肽（ＹＩＧＳＲ）对胃癌细胞系ＭＧｃ－８０３的体外抑制作用，在多肽浓度为１ｍｇ／ｍｌ时抑制率高达７３％．抑制作用的机理可能是Ｌａｍｉｎｉｎ五肽竞争了肿瘤细胞上Ｌａｍｉｎｉｎ受体，从而封闭了肿瘤细胞对基底膜的结合及增殖．因此，利用Ｌａｍｉｎｉｎ五肽一类的药物有可能为肿瘤术后防止扩散提供一个新方法．     

抗孕酮化合物RU486的研究

该文对ＲＵ４８６的合成路线、结构特征、与ＰＲ作用的构效关系和作用特点作了介绍。     

目前内分泌研究进展

本文对传统内分泌腺之外的一些细胞和组织的内分泌功能作一概述，其中包括神经内分泌，胃肠道内分泌，血循环系统内分泌，免疫细胞内分泌及排泄系统内分泌，研究表明，人体所有的细胞事实上都具有产生激素的共同基因，都有合成和分泌激素的能力，只是他们在不同细胞表达的方式和数量不同而已。人们预言，内分泌功能是一切生命细胞最基本的特征之一，所有的细胞都具有内分泌功能。     

枣快速繁殖技术研究

通过三年对枣树组织培养的研究和实践应用，结果表明，适宜的灭菌时间为７５％的乙醇５．７ｓ，０．１％升汞１０ｍｉｎ。适宜的激素配比为６－ＢＡ３４ｍｇ／Ｌ＋ＮＡＡ０．０１ｍｇ／Ｌ，适宜生根的激素为ＩＢＡ０．４－０．６ｍｇ／Ｌ。     

煤微生物转化产物对色素微生物和蔬菜的刺激作用

在两种产色素微生物的培养基中，加入煤的微生物转化产物，所得微生物发酵液的色素浓度及吸光度有较显著的增加，将煤生物转化产物作为蔬菜生长刺激素的实验亦取得了显著效果，该结果揭示煤微生物转化产物在生物激素上的重要应用前景。