白芨微球的研制及其肝动脉栓塞实验研究

研究白及微球的制作工艺及其猪肝动脉栓塞和临床患者栓塞的效果。方法：采用乳化－冷凝技术制备白及微球,并通过正交实验,确定制备特定粒径微球的最佳工艺条件。通过股动脉经导管把白及微球注入肝动脉。结果：正交试验结果表明所考察的因素如搅拌速度、油／水比例和白及胶浓度对微球粒径及分布均有显著影响。白及微球呈圆形,粒径分布窄,圆整,分散性好,平均粒径为５４３６μｍ。实验小猪微球栓塞后的肝动脉造影表明白及微球可致肝右支动脉一级、二级分支完全栓塞;病理检查表明梗死区可见肝组织呈肝硬变改变,大量的假小叶形成。微球临床初步应用效果良好。结论：白及微球作为新型肝动脉栓塞制剂在介入栓塞治疗中有较好的作用     

Hepatic glucose signals vagally modulate the cyclicity of gastric motility in rats

The cyclicity and intensity of gastric motlity were examined following glucose injection into the portal vein with an intragastric balloon in anesthetized rats. Enhanced gastric motility caused by insulin administration was influenced by 4 mM glucose (25 l) injected into the portal vein; glucose provoked a shift in the cyclicity power spectrum without any change in intensity. The peak power spectrum shifted from 4.0–5.0 cpm to 2.0–3.0 cpm. Hepatic branch vagotomy abolished the response.The results suggest that glucose signals in the hepatic vagal branch modulate the cyclicity of gastric motiligy.     

肝癌介入治疗现状

为探讨肝癌目前的治疗现状,综合国内外近10 a来已公开发表的论著、文献阐述肝癌介入治疗的现状,归纳总结文献中关于肝癌介入治疗的不同方法,比较各自的优劣.认为肝癌介入治疗方法较多,目前缺乏规范,主张综合治疗为主,实施个体化方案.     

新生猪肝细胞输注治疗大白鼠急性肝功能衰竭的研究

本文报导以新生的猪肝细胞输注治疗D—氨基半乳糖致急性肝功能衰竭的大白鼠,并对其机理作了初步探讨。腹腔输注新生猪肝细胞能使发病大白鼠的存活率由对照组的16.22％提高到55.56％,用放射性同位素技术证明输注肝细胞能使病肝细胞的H-thymidine掺入量明显增加,病肝再生加快,从而使病鼠恢复健康。     

抗大鼠肝脏脂酶抗血清的制备及应用

按照ＲＨＬｃＤＮＡ核苷酸序列所预示肽链的氨基酸序列，化学合成了与肽链Ｎ端、Ｃｕｍｄｊｔ ｋ ｕｋ ｓｈ ｃｆ ｙｉｄ ｒ ３ｗｈ ｅｅｄｙ     

TSGF与AFP对原发性肝癌的诊断特异性、灵敏度分析

分析探讨TSGF与AFP检测在原发性肝癌诊断中的特异性和灵敏度,以及对原发性肝癌的早期诊断价值.采用分光光度法检测TSGF,包括87例原发性肝癌(男性54例、女性33例、平均年龄67岁)、67例继发性肝癌(男性42例、女性25例、平均年龄63岁),同时用化学发光法检测AFP.对照检测179例肝病患者的TSGF、AFP(肝硬化患者74例、慢性乙型肝炎105例).其结果TSGF对原发性肝癌的敏感性(86.2%)高于AFP(71.4%)有显著性差异(P＜0.05);TSGF对继发性肝癌的敏感性(88.1%)高于AFP(35.8%)有显著性差异(P＜0.05);二者联合检测敏感性提高至94.3%.由此证明TSGF对恶性肿瘤的检测兼有早期性和广谱性,对原发性肝癌其灵敏度高于AFP,特异性低于AFP.二者联合检测显著提高对原发性肝癌诊断的特异性和灵敏度,对原发性肝癌早期诊断有重要的参考价值.     

Subcutaneous transposition of the spleen enhances the survival rate following 90% hepatectomy in rats

Summary The effects of subcutaneous transposition of the spleen (STS) on the survival rate following 90% hepatectomy were investigated in rats. The survival rate was significantly higher in the STS group than in the non-STS group. Light microscopy enabled us to note that congestion in the terminal portal veins and sinusoids occurred either slightly earlier or to a higher degree in the non-STS group.     

Postischemic ATP levels predict hepatic function 24 hours following ischemia in the rat

Summary Hepatic function was assessed by the aminopyrine breath test (ABT) in male Sprague Dawley rats 24 h after partial hepatic ischemia. ABT decreased progressively to 26.3 (p<0.05) and 19.7% of dose (p<0.05) after 90 and 120 min of ischemia, respectively. ABT at 24 h after injury was correlated to the concentration of ATP in the ischemic lobes 1 h after the onset of reperfusion (r²=0.971) but not to ALT activity in plasma at 1 h (r²=0.391). We conclude that postischemic ATP levels are a better index of subsequent hepatic function than ALT.     

肝内门—腔静脉分流术的应用解剖

目的：为临床进行肝内门—腔静脉分流术提供解剖学基础。方法：在31例成人肝标本上观测肝静脉的汇合类型、开口纵径、横径,肝静脉与门静脉交叉处的长度、横径、两静脉间距离。结果：三条肝静脉注入下腔静脉分三种类型,肝右静脉与门静脉右干及右后上支交叉、肝中、左静脉与门静脉左干交叉。结论：肝内门—腔静脉分流术的首选部位是在肝右静脉与门静脉右干及右肩上支之间;其次是在肝中静脉与门静脉左干之间;肝左静脉不宜行肝内门—腔静脉分流术。