Cooperative inhibitory effect of follicular fluid and cAMP on hamster oocyte maturation

Summary Porcine or human follicular fluid inhibited the spontaneous maturation of isolated hamster oocytes in vitro during the first 1.5 h of culture. Moreover, the presence of 50% follicular fluid combined with 100 M dbcAMP cooperatively reduced the incidence of germinal vesicle breakdown. The addition of FSH also inhibited the resumption of meiosis, and the presence of LH did not overcome the inhibitory effects of follicular fluid and tended to impede isolated hamster oocyte maturation in vitro.     

三种脑-肠肽对人绒毛膜促性腺激素分泌的影响

本工作利用灌流技术观察了3种脑-肠肽(P物质,CCK及NT)对新鲜人工流产的绒毛hCG分泌的影响。结果表明,P物质,CCK及NT均明显刺激绒毛释放hCG,并发现CCK作用大于P物质及NT。提示,hCG的分泌可能受这3种脑-肠肽的调节。     

Hypothalamic tyrosine hydroxylase activity,plasma gonadotropin and prolactin levels after aminooxyacetic acid in ovariectomized rats

Summary Plasma concentrations of gonadotropin, prolactin and hypothalamic tyrosine hydroxylase (TH) activity were measured in ovariectomized rats treated with aminooxyacetic acid (AOAA), a drug which elevates brain GABA levels. Hypothalamic TH activity was significantly increased with a significant decrease in prolactin (Prl) release. Plasma levels of gonadotropins were not modified by AOAA. These results support an inhibitory action of GABA on Prl release possibly mediated through hypothalamic dopamine.Supported by grants from Indian Council of Medical Research (ICMR), New Delhi. RIA kits for the estimation of LH, FSH and Prl were kindly supplied by Dr A.F. Parlow, NIAMDD-NIH, Bethesda, Maryland, USA. GNB is a UGC research fellow.     

人类体外受精助孕治疗中多原核受精的相关因素分析

收集2011年7月至2012年11月在广西生殖医学研究中心行体外受精助孕治疗的456例患者资料,将观察到多原核受精的235例作为多原核受精组,未观察到多原核受精的221例作为无多原核受精组,比较两组间基础卵泡刺激素(基础FSH)、黄体生成素(LH)水平、窦卵泡数(AFC),促性腺激素(Gn)总剂量及天数,HCG(人绒毛膜促性腺激素)日血清雌二醇(E2)水平、HCG日血清孕酮(P)水平、HCG日血清黄体生成素(LH)水平,HCG日大卵泡(≥14mm)数,获卵数,受精密度等助孕治疗相关指标的差异,分析人类体外受精中多原核受精的影响因素。结果提示,Gn用量小,HCG日血清E2水平较高,卵泡数及获卵数较多,受精率较高的周期中出现至少1个多原核受精合子的可能性比较大。     

绒毛膜促性腺激素对人外周T淋巴细胞转化的影响

目的;探讨人绒毛膜促性腺素（human chorionic gonadotropin,HCG）在神经内分泌－免疫网络中的作用。方法：应用同位素示踪法体外观察HCG以人外周T淋巴细胞转化增殖反应的影响。结果：HCG浓度在7．5－125U／mL时对植物凝集索刺激的T淋巴细胞转化增殖反应有显著抑制作用（P＜0．001）。结论：HCG作为一种负性调节因子,通过对T淋巴细胞的抑制作用,可能在神经内分泌－免疫调节网络中起重要作用,不仅参与妊娠时机体对胎儿的免疫相容机制,而且与肿瘤患者体风的免疫抑制机制有关。     

从噬菌体抗体库中淘选人绒毛膜促性腺激素的单链抗体

由鼠源噬菌体抗体库淘选到展示有人绒毛膜促性腺激素单链抗体的噬菌体克隆。用ELISA方法进行检测,从结合力最强的克隆中分离单链抗体基因,插入经改造的高效表达载体pET-21a(+)中,转化大肠杆菌,诱导表达。从培养基中可检测到可溶性抗人绒毛膜促性腺激素的单链抗体。     

HCG的生物电化学免疫分析

共价耦联HCG抗体的醋酸纤维素膜,当表面发生抗体与抗原复合的免疲化学反应后、膜的电荷密度向负值减小的方向移动,膜电位随抗原浓度的增大,向正方向移动。根据膜电位与抗原浓度之间的关系,可构成测定HCG的膜电位免疫传感器。利用夹心原理,在膜表面形成抗体-抗原-酶标抗体的夹心结构所组成的酶免疫传感器,具有很高的检测灵敏度,且与放射免疫测定显著相关。     

采用染料亲和层析的方法纯化卵泡刺激素

采用染料亲和层析、QAE离子交换层析等纯化步骤，从绝经期妇女尿促性腺激素(HMG)粗品中分离纯化获得高纯度卵泡刺激素(FSH)产品，其中FSH的生物活性达到268.1IU／mg，卵泡刺激素与黄体生长素(LH)的活性比值达到259，产品超过欧洲药典对高纯度卵泡刺激素的要求。此工艺FSH的活性回收率高达65％，而且分离纯化步骤少，简单易行，适合于大规模生产。     

重组质粒pPIC9K-pZP3α-hCGβ CTP109～145的构建

目的：为发展多特异性避孕疫苗,制备重组抗原pPIC9K－pZP3α－hCGβCTP^109-145,用基因工程技术构建重组质粒pPIC9K－pZP3α－hCGβCTP^109-145,方法：根据pZP3α全长和hCG 0633CTP109-145编码的DNA序列设计两对引物,通过部分重聚合酶链式反应（overlaping PCR）,扩增出pPIC9K－pZP3α－hCGβCTP^109-145片断,克隆到载体质粒pPIC9K中,然后导入大肠杆菌DH5α,用PCR和双酶切反应鉴定重组质粒,并用DNA测序仪对重组质粒测序,结果：3步PCR扩增出pPIC9K－pZP3α－hCGβCTP^109-145DNA片段,插入到载体质粒pPIC9K的克隆位点,获得重组pPIC9K－pZP3α－hCGβCTP^109-145表达质粒,测序结果显示插入序列与设计预期完全一致。结论：重组质粒pPIC9K－pZP3α－hCGβCTP^109-145构建成功,为表达重组抗原pPIC9K－pZP3α－hCGβCTP^109-145,探讨重组多特异性避孕疫苗抗生育效能的研究建立基础。