抗人绒毛膜促性腺激素（HCG）单克隆抗体的研制和特性分析

以HCG为抗原,免疫BALB/c小鼠,取其脾细胞与小鼠Sp2/0—Ag14骨髓瘤细胞融合,经反复筛选和再克隆化,获得8株能稳定分泌抗HCG特异单克隆抗体的杂交瘤细胞株。对其中6个高效价单抗进行了特异性和抗原决定簇检测,选出两个抗相距较远不同决定簇的单抗(2D_4,4F_4)作进一步特性分析。结果显示:免疫球蛋白亚类鉴定,2D_4为IgGl亚类,4F_4为IgG2a亚类;亲和力常数(K_(affi)),2D_4为5.41±0.94×10~(-10)mol/L,4F_4为1.37±0.33×10~(-9)mol/L。证实该两种单克隆抗体可用于研制有关临床诊断试剂盒。     

Detection of Messenger RNA for Gonadotropin-Releasing Hormone (GnRH) but not for GnRH Receptors in Rat Pancreas

IntroductionGonadotropin- releasing hormone ( Gn RH) ,whichis synthesized in the brain and secreted into theanterior pituitary,plays a central role in thecontrol of mammalian reproduction[1,2 ] .Gn RH andGn RH m RNA have been reported to exist in non-hypothalamic tissues such as ovary[3,4 ] ,breast[5] ,testes,and placenta[6] .The Gn RH binding siteand its m RNA have also been found inextrapituitary tissues,including gonads andplacenta[7] .These findings indicate the biologicalsignifican…     

HMG在家兔超数徘卵中的应用

用国产的尿促性腺激素(HMG)，孕马血清促性腺激素(PMSG)，人绒毛膜促性腺激素(HCG)，对40只母兔进行超数排卵处理试验。结果显示，国产的HMG对母兔有良好的超排作用。试验兔中有90％表现出超排效应，平均每只母兔可回收到30．2个胚胎，最多的一只母兔回收到103个胚胎，对照组(PMSG)平均为7．6个胚胎，差异十分显著(P＜0．01)，同时显示季节对超数排卵的影响也很大，春季优于秋季。