带有绿色荧光蛋白基因(gfp)的植物重组表达质粒pBI-GFP的构建

利用 DNA重组技术 ,将经定点突变改造的绿色荧光蛋白基因 ( gfp)克隆到植物表达载体p BI-1 2 1中 ,成功地构建了植物重组表达质粒 p BI-GFP.     

Transferring gfp gene with ion implantation and transient expression of gfp in liliaceous pollen cells

Liliaceous pollen cells were implanted by 4.0 MeV C2+ ion beam or by 25.0 keV N+ ion beam. Laser confocal scanning microscopy (LCSM) of the implanted intact samples showed that parts of the implanted pollen cells could be stained by propidium iodide (PI). This indicated that energetic ion beam could directly act on cells beneath the pollen coats and made channels for entry of the molecules from outside of the cells. LCSM analysis of green fluorescent protein (GFP) showed that energetic ion beam could mediate transient expression of gfp in treated pollen cells. Compared with 25.0 keV N+ ion beam, implantation of 4.0 MeV C2+ ion beam greatly improved gene transfer efficiency in pollen cells.     

Transferring gfp gene with ion implantation and transient expression of gfp in liliaceous pollen cells

Anewtechniqueofgenetransfermediatedbyenergeticionbeamirradiationorimplantationwasde velopedinrecentyears .Stokeretal.[1] foundthatthesusceptibilityofcricetidaeembryocellstoaden ovirustransformationwassignificantlyimprovedbyX rayradiation .Stevensetal.[2 ] andZengetal.[3]foundthatpreliminarytransferrateofplasmidDNAcouldbeimprovedtoalargedegreebyionizingradia tion .UV[4 ] andenergeticheavyions[5] werefoundtohavesimilareffects ,whichindicatedthationizingra diationcouldmakeiteasyforextraneousDN…