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裂殖壶菌Schizochytrium sp.FJU-512是高产二十二碳六烯酸(DHA)的一株海洋真菌,其细胞内油脂占细胞干质量的18.3%.细胞油脂的总脂肪酸组成为45.7%DHA,12.5%DPA和41.8%棕榈酸.总油脂由26.4%极性脂和73.6%中性脂组成.薄层色谱、高效液相色谱及质谱分析表明该菌株还能合成强抗氧化剂虾青素.采用均匀设计获得高产DHA的最优培养基(g/L):葡萄糖109.7,蛋白胨19.2,酵母膏20.0,海水晶10.4,DHA产量高达2.10 g/L. 相似文献
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Long chain polyunsaturated fatty adds, i. e., docosahexaenoic acid (DHA or C22 : 6n - 3), amchidonic acid (AA or C20 : 4n -6) have been identified as essential fatty acids and play an important role in growth and development of infants. Measurement of fatty acid composition is usually by collection of blood, but to obtain blood in infants is difficult. Nowadays, the fatty acid composition can be estimated by collecting buccal mucosal cells, which can avoid repeated blood sampling. The of this paper is to compare the fatty acid composition of cheek cells with that of plasma and red blood cells (RBCs). In this study, twenty-seven infants were enrolled, and buccal mucosal cells and blood samples were obtained from these infants of the same time. Fatty acid composition of buccal mucosal cells, plasma and RBCs were measured by capillary gas chromatography. The results show that the contents of AA and DHA in the buccal macosal cells are correlated well with that in the plasma [r=0.36 (P=0.042) and r =0.38 (P =0.033), respectively]. The ratio of AA to DHA is 1.32% in buccal mucosal cells, 1.60% in plasma and 1.55% in RBCs and there are no significant differences among groups (P = 0.134). It shows that the fatty acid composition in buccal macosal cells can reflect the fat nutrition status in infants and can be detected by capillary gas chromatography. Estimating fatty acid composition of buceal mucosal cells in infants by capillary gas chromatography is feasible, and because of its noninvasiveness, it can be suitable for nutrition research in infants. 相似文献
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龚坤华 《广西师范学院学报(自然科学版)》1994,(1)
本文论述了利用变压器油的气相色谱分析技术判断变压器内部潜伏性故障的原理,并就典型实例通过使用几种不同方法的判断,验证其准确性.显示了使用该技术预测、判断变压器故障的重要性。 相似文献
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本文提出了一种鉴定厌氧菌的新方法——色谱/红外光谱联用方法,该法由色谱部分实现对混合组分的分离,由红外部分实现对单一组分的定性、定量分析.文中讨论了以某两波长处的吸光度比值作为定性指标的定性方法,探讨了对编码重复的菌种的再区分. 相似文献
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