基于DNA-AuNCs的荧光传感新方法灵敏检测胰蛋白酶活性

以DNA模板金纳米簇(DNA-AuNCs)作为荧光探针,发展了一种灵敏检测胰蛋白酶活性的荧光分析新方法.在胰蛋白酶存在条件下,牛血清白蛋白水解成多肽片段,释放出游离的半胱氨酸残基.半胱氨酸残基与金纳米簇通过Au-S键形成非荧光的配合物,导致金纳米簇的荧光强度显著降低.该方法对胰蛋白酶的线性检测范围为0.1～2.0mg/L,检出限为20μg/L(R_(SN)=3).此外,该方法被成功应用于人血清样品中胰蛋白酶含量的测定.     

高校图书馆不同功能定位下对音乐元素运用的思考

在高校图书馆传统功能定位下，音乐元素的融入受到了极大的限制。音乐及阅读心理治疗等新功能的设置，则将音乐元素更广、更深地融入到高校图书馆的工作中，可更好地为高校广大师生的学习生活提供服务。从高校图书馆传统功能定位下音乐元素的融入和高校图书馆新功能定位下音乐元素的融入两个方面，提出了高校图书馆不同功能定位下对音乐元素运用的思考。     

高速实时可见光通信技术研究及应用

 介绍了可见光通信技术的发展历史及研究现状。基于带宽拓展技术搭建了1 W荧光型LED做光源、PIN光电二极管做探测器、单路速率610 Mb/s的实时传输演示系统，该系统在传输距离为6.2 m时的误码率为3.48×10^-5。在此基础上搭建了荧光型LED为光源、PIN光电二极管做探测器的、双向100 Mb/s无线光上网演示系统。     

利用Wittig反应合成荧光增白剂DX.EB

基于Wittig反应原理,利用(邻氰基苄基)膦酸二甲酯、(对氰基苄基)膦酸二甲酯、对苯二甲醛和甲醇钠合成了荧光增白剂DX.EB,研究了合成工艺.当原料(邻氰基苄基)膦酸二甲酯∶(对氰基苄基)膦酸二甲酯=1∶1.5,40℃滴加甲醇钠时,收率为最佳,可达74.72%.     

Synthesis and Photophysical Properties of Novel Rod-shaped and Star Shaped Fluorescent Phosphane Derivatives

1 Results Over the past few years significant research has been directed toward the development of organic materials for potential application in molecular photonic devices[1] and the development of sensors[2].Interest in these materials is primarily due to the infinite numbers of possible molecular structures with the desired properties,by virtue of the tremendous capabilities of organic synthesis.For the development of a new fluorescent molecular sensor consisting of a recognition moiety linked to a f...     

多平台基于方位时延差的目标运动分析

以拖曳阵列与本舰所构成的声纳系统为例 ,从双平台观点考虑 ,利用对目标的两方位及时延的量测为依据 ,研究了该系统的目标运动分析 (targetmotionanalysis,TMA)问题。采用了最大似然估计、扩展Kalman滤波(EKF)方法 ,实现了对目标运动参数的估计 ,并给出了它们的Cramer Rao低界 (CRLB)。通过仿真考察了参数变化对估计精度的影响。仿真结果表明上述方法具有较高定位精度。该方法适合于主、被动形式的多平台系统 ,且容易实现。     

检验唐氏综合症的法医学方法研究

利用荧光标记的PCR法，对唐氏综合症的检验进行研究．采用法医学检验常用试剂盒AmpFe STR^TM Profiler Plus^TM对唐氏综合症病例进行PCR扩增，扩增产物用ABIPRISMT”310型DNA全自动测序仪分型判断．结果表明，该检验方法具有简便、快速、直接、准确的特点，检验效果非常好，而且可用于产前诊断．该方法用于检验唐氏综合症是一种理想的方法．     

In vitro amiodarone protein binding and its interaction with warfarin

Summary The binding of amiodarone to human plasma protein and to bovine serum, albumin was studied by three different methods, ultracentrifugation, equilibrium dialysis and fluorescence spectroscopy. The fraction of amiodarone bound to plasma protein amounted to 96.3%. The changes in the binding properties of 1-anilino-naphthalene-8-sulfonate for bovine serum albumin using warfarin and amiodarone as independent inhibitors were analyzed in terms of binding site specificity. The findings indicated that amiodarone and warfarin have two different binding sites on bovine serum albumin, so a noncompetitive inhibition mechanism was indicated. On the basis of our data we cannot exclude other mechanisms of interaction besides direct displacement of one drug by another; nevertheless, metabolite interference between amiodarone and coagulation cofactors may better explain the enhancement of warfarin's pharmacological action in association with amiodarone.This work was partially funded by the CNR (National Research Council, Rome, Italy), Program on Clinical Pharmacology and Rare Diseases. The authors would like to thanks Drs E. Marzi and E. riva for their help.     

Relationship between the partitioning properties of motoneuron recruitment and the needle feeling propagation

Histological and physiological studies indicated that most skeletal muscles can be divided into a series of relatively independent sub-volumes: "neuromuscular compartments" and the partitioning property of the muscle result in the localization of muscle reflex. In the present experiment we studied the recruitment properties of medial gastracnemius (MG) muscle motoneuron pool of decerebrate cat with two kinds of local mechanical stimulation: local stretch and acupuncture-like stimuli. The results indicate that: (ⅰ) there is an obvious property of localization of recruitment activity, only the MNs which innervate the stimulated compartment were recruited by weaker and shorter stimuli; (ⅱ) recruitment activity spread to those MNs which supply the adjacent and distal compartments during the strength of stimulation or duration of the stimulation was increased; and (ⅲ) the recruitment property of muscle activity elicited by the local mechanical stimulation is thought similar to that of "needle feeling" along the meridian pathway during stimulation of acupuncture point.     

胰腺腺泡细胞胞浆游离钙浓度的测定及药物作用的研究

本文建立了用荧光指示利Fura－2测定胰腺腺泡细胞胞浆游离Ca2＋浓度（［Ca2＋］i）的实验技术,包括：胰腺腺泡的分离制备;Fura—2／AM的负载;腺泡悬液的荧光光谱测量等．测定了胰腺腺泡细胞［Ca2＋］i。的静息值和卡巴可促腺泡细胞［Ca2＋］i升高的剂量—效应关系及动力学过程,并研究了柴胡总皂甙对胰腺腺泡细胞［Ca2＋i］的影响．发现0．01mg／ml浓度的柴胡总皂甙使胰腺腺泡细胞［Ca2＋］。明显升高,0．1mg／ml浓度时可使［Ca2＋］。升高到静息值的3倍,提示柴胡总皂甙的促酶分泌作用是通过胞内［Ca2＋］i升高介导的．