检验唐氏综合症的法医学方法研究

利用荧光标记的PCR法，对唐氏综合症的检验进行研究．采用法医学检验常用试剂盒AmpFe STR^TM Profiler Plus^TM对唐氏综合症病例进行PCR扩增，扩增产物用ABIPRISMT”310型DNA全自动测序仪分型判断．结果表明，该检验方法具有简便、快速、直接、准确的特点，检验效果非常好，而且可用于产前诊断．该方法用于检验唐氏综合症是一种理想的方法．     

时变条件下有宵禁限制的最短路问题

在组合优化过程中,往往需要获得从起点到终点之间的最短路,而其所考虑的目标可能是一个与时间相关的变量.同时,对于网络中的节点往往有宵禁的限制(curfews).给出时变条件下有软、硬宵禁限制的最短路模型,设计了求解时变条件下有宵禁限制最短路的算法,并对算法的复杂性进行了分析.最后给出了一个应用算例.     

In vitro amiodarone protein binding and its interaction with warfarin

Summary The binding of amiodarone to human plasma protein and to bovine serum, albumin was studied by three different methods, ultracentrifugation, equilibrium dialysis and fluorescence spectroscopy. The fraction of amiodarone bound to plasma protein amounted to 96.3%. The changes in the binding properties of 1-anilino-naphthalene-8-sulfonate for bovine serum albumin using warfarin and amiodarone as independent inhibitors were analyzed in terms of binding site specificity. The findings indicated that amiodarone and warfarin have two different binding sites on bovine serum albumin, so a noncompetitive inhibition mechanism was indicated. On the basis of our data we cannot exclude other mechanisms of interaction besides direct displacement of one drug by another; nevertheless, metabolite interference between amiodarone and coagulation cofactors may better explain the enhancement of warfarin's pharmacological action in association with amiodarone.This work was partially funded by the CNR (National Research Council, Rome, Italy), Program on Clinical Pharmacology and Rare Diseases. The authors would like to thanks Drs E. Marzi and E. riva for their help.     

AnAnolis skin melanophore assay suitable for photoaffinity labeling studies with α-MSH

Summary Visual determination of MSH-induced pigment migration in melanophores of small pieces ofAnolis carolinensis skin is standardized by first measuring photoelectrometrically the change in reflection/transmission of the whole dorsal skin in response to different hormone concentrations. This method allows the rapid and precise recording of time-response curves after photoaffinity labeling of MSH receptors or of dose-response curves of large series of synthetic compounds.Acknowledgments. We wish to thank Ms V. Jäggin, Ms C. Schulthess and Ms G. van Hees for excellent technical assistance, Dr. R. Andreatta, Ciba-Geigy AG, Basel, for his generous gift of -MSH and Prof. A Pletscher for his continuous interest. This work was supported by the Swiss National Science Foundation.     

溶剂效应对中氮茚荧光性质的影响

合成了一系列中氮茚化合物，比较了它们在不同有机溶剂中紫外光谱和荧光光谱的变化情况，探索了化合物的微观结构与其光学性能之间的某些关系，结果表明溶剂极性对该类化合物吸收光谱的影响不明显，但对化合物的Stokes位移、荧光强度以及荧光量子效率的影响都较大，中氮茚化合物分子的荧光特性首先取决于它自身的分子结构，特别是各种能增强系间穿越过程的取代基的存在，而外界因素，如介质，温度、压力、浓度、酸度等对试剂荧光特性也有很大影响．     

Real time observation of the photocleavage of single DNA molecules

A method for real time observation of photo-cleavage of stratched λDNA at single molecular level by a fluorescent microscope coupled with CCD is developed.DNA molecules stained with YOYO-1 are stretched by the mo-lecular combing technique and fixed on a modified slide.Then the Process of Photocleavage and relaxation of DNA under radiation of blue light is observed.We speculate that the conformation change of stretched DNA and the effect of water are likely to facilitate the effect of YOYO photocleav-age DNA molecules.The photocleavage effect of YOYO for stretched DNA may be useful to study DNA elasticity,cancer research as well as the interaction between DNA and dyes.     

Design and application of 60mer oligonucleotide microarray in SARS coronavirus detection

In April 2003, a novel coronavirus[1,2] which was associated with cases of Severe Acute Respiratory Syn-drome (SARS) was first isolated and sequenced in Canada. The genome of SARS coronavirus (SARS-CoV) is 29727[3] nucleotides in length and has 11 known open reading frames (ORFs). Although the genome organiza-tion of this virus is similar to that of other coronaviruses, phylogenetic analyses and sequence alignment show that SARS-CoV is not closely related to any of the previously ch…     

基于MSC1162A驱动芯片的VFD显示模块的设计

介绍了基于MSC1162A驱动芯片和PIC16F73设计的VFD显示模块,实现了VFD的动态显示,介绍了VFD显示模块的显示驱动方法和SPI数据快速通讯应用.其产品已经批量生产并具有良好的实用性和稳定性.     

一个特殊的顺式发夹核酶对感染烟草原生质体的影响

研究一个烟草花叶病毒(TMV)cDNA3′末端被特异切割的发夹核酶对感染原生质体的作用。选用一个外源基因绿色水母荧光蛋白(GFPuv)作为报道基因，取代了大部分的CP编码区域，以研究在烟草原生质体中发夹核酶对TMV载体的复制，通过GFP的表达来确定表达量。顺式发夹核酶对TMV载体感染效率的影响通过体外转录来观察。TMV GFPRIB体外转录感染原生质体的效率比与其不含该核酶的对应物的感染效率高3～5倍。Northern杂交结果表明，包括核酶在内的3’末端非病毒序列在体外转录反应开始后就被顺式发夹核酶切除了。当直接用载体DNA pSTMVGFPRIB感染原生质体时，感染原生质体的数量比与之相对应的pSTMVGFP NOS感染原生质体数多50％～80％。     

荧光动力学分析法测定5种酮清除超氧自由基和羟基自由基的活性

利用荧光动力学分析法研究了5种酮化合物对超氧自由基(O2.-)和羟基自由基(.OH)的清除作用.利用邻苯三酚自氧化产生O2.-;利用Fenton反应产生.OH,向这2个反应体系中分别加入5种口山酮化合物,O2.-和.OH的产生速率明显降低.说明5种酮化合物对和有较强的抑制作用.探讨了酮化合物的结构与其抗氧活性的关系.