Molecular cloning and expression of interleukin Ibeta (IL-1β) from red seabream (Pagrus major)

The interleukin 1β (IL-1β) cDNA was cloned from the red seabream (Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1β sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1β (RS IL-1β). The sequence contained 1252 nucleotides that included a 5' untranslated region (UTR) of 84bp, a 3' UTR of 410 bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pI of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1 family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1β genes. The RS IL-1β had the highest homology with piscine IL-1β according to phylogenetic tree analysis.     

低频声波定向技术研究

应用声呐基阵的设计理论，设计了一种圆柱形组合脉动球源阵。对此声源阵低频声波声场的远场进行了理论分析与仿真研究，表明此声源阵对低频声波具有指向性，通过调整此组合声源阵的结构，可改善对低频声波的指向性。     

水稻苯达松敏感致死基因的RAPD标记的克隆及测序

用3S Spin DNA Agarose Gel Purification Kit试剂盒将与水稻苯达松敏感致死基因相连锁的RAPD遗传标记S20—420和S316—600回收纯化，连接于pGEM—T载体并克隆测序，得到了S20—420和S316—600的全序列，其长度分别为423bp、606bp．将两端序列设计特异PCR扩增引物可用于检测水稻苯达松敏感致死基因和标记辅助育种．     

多自适应波束天线节点的最优组网拓扑控制

自适应波束天线由于其发射接收具有强指向性的特点,其网络拓扑控制问题、方法与全向天线存在较大不同。针对节点安装有多个空间均匀分布的自适应波束天线进行组网通信时的拓扑优化控制问题,建立了自适应波束天线和网络节点通信的数学模型;对拓扑优化控制的问题进行了分析,其问题本质是对节点安装的多部天线进行最优化链路分配。以网络通信质量的量化计算作为目标函数,提出了一种基于0-1规划的拓扑控制优化模型,并对网络连通性进行了分析,给出了两节点连通以及整个网络连通的充要判决条件。最后给出了2种不同节点数情况下的仿真计算结果,并通过穷举法验证了模型计算结果的正确性。     

Cloning and expression of the vp39 gene ofBombyx mori nuclear polyhedrosis virus inE. coli

The nuclear capsid protein gene (vp39) ofBombyx mori nuclear polyhedrosis virus (BmNPV) was amplified successfully by PCR technique and inserted into pGEM 3zf(+). The 5′ and 3′ terminal area of the amplified vp39 gene were sequenced with silver-staining dideoxy method. Bmvp39 gene was sub-cloned into the expression vector pRSET-A, and transformed intoE. coli BL21. This gene was highly expressed by IPTG induction. SDS-PAGE analysis showed that the expressed protein is about 38 kd, and the expressed amount reached maxium in 4 h with IPTG induction. Supported by the National Natural science Foundation of China and the Doctoral Foundation of Edn carto Committee Liu Deli: born in 1954, Doctoral Candidate from Huazhong Normal University To whom correspondence should be addressed: (027-7882712-2938)     

Molecular cloning and nucleotide sequence of classical swine fever virus strain Shimen

According to the previously published CSFV sequences, 18 paris of partially overlapping primers which span the entire genome of CSFV strain Shimen were designed and synthesized. Each cDNA fragment of strain Shimen was amplified by RT-PCR method from the anticoagulant blood of strain Shimen infected pig. The PCR fragments were cloned into pGEM-T vector respectively and sequenced. The results show that we have obtained the nucleotide sequence of strain Shimen. The viral RNA consists of 12 297 nucleotides including noncoding regions of 373 and 227 bases at the 5′ and 3′ end, respectively, and a single large open reading frame spanning 11 697 nucleotides in the middle, which encodes an amino acid sequence of 3 989 residues with a calculated molecular weight of 437.6×10³. The precisely sequencing of 5′ and 3′ termini is undertaking. Supported by the National Pandeng Project Huang Qianhua: born in 1968. Graduate student     

等导重根特征对的泰勒展开

讨论多参数非亏损特征对的可微性,推导了具有等同一阶偏导数的重根特征的计算公式,进而得到了它们的泰勒开展开式。     

血小板反应素-1活性片段的克隆与表达

选择两段血小板反应素（TSP-1）中抑制血管再生的活性片段，设计两对引物，使用RT-PCR的方法从人血细胞中进行克隆并对获得片段进行测序验证．克隆片段大小分别为723和522bp，命名为聪P-1-1和聪P-1-2．利用原核表达载体pET-29a获得大肠杆菌重组子，重组子经过IPTG诱导以包涵体的形式表达相应的多肽片段．再对包涵体进行体外溶解、纯化，得到了目的多肽．     

关于preinvex函数和pseudoinvex函数

本文讨论了可微的强invex函数和强pseudoinvex函数分别与其梯度的强不变单调和强不变伪单调的关系,得到了pseudoinvex函数在某些条件下可以等价prequasiinvex函数.证明了:若f关于向量值函数η是preinvex函数,且满足lipschitz条件,则y为f(x)的全局极小点等价于0∈(e)0f(y).     

基于3D-GM-BPM的平行定向耦合器模拟研究

提出了基于伽辽金法的三维光束传播法（3D－GM－BPM），可直接应用于三维波导光电子器件的光波传输模拟，并用它模拟分析了基于脊形光波导的平行定向耦合器遥光波传输特性。3D－GM－BPM最终归结为求角一阶常微分方程组，导出矩阵小，计算效率高。另外，平面映射边界条件使该方法无须引入人工边界条件，因而消除了非物理反射，有较高的计算精度。