植物环肽

首先简要地介绍了植物环肽的定义、研究历史、结构分类、生物活性与生物功能等,最后以石竹科类型环肽和环蛋白为例详细介绍其检测方法、结构研究方法、化学合成或生物合成等.     

Application of a TLC chemical method to detection of cyclotides in plants

Iodine, Dragendorff＇s reagent, ninhydrin and Coomassie brilliant blue G-250 reagents were used to detect cyclotides from proteins, cyclopeptides, linear-peptides and amino acids in the thin layer chromatography （TLC）. Cyclotides could be distinguished from the others by comparing the TLC coloring plots with G-250 and ninhydrin reagents. With this method, cycloUdes were discovered in Viola labridorica, V. tricolor, V. hamiltoniana, Momordica charantia, and M. cochinchinensis and over 10 cyclotides were isolated, three of which were determined as known cyclotides cycloviolacin O2, kalata B1 and vary peptide A.