体外重组细菌光敏色素AphA修饰sBLM的光响应

通过体外重组获得了鱼腥藻Anabaenasp．PCC7120细菌光敏色素AphA，进而将其对金属支撑型BLM进行修饰．实验发现，细菌光敏色素修饰的BLM对于红光(650nm)和远红光(700nm)具有不同的光响应信号．当用远红光照射时，产生一个正向的电流响应，用红光照射时，产生一个反向的电流响应．     

Sequence analysis of the Microcystis aeruginosa FACHB-912 phytochrome gene supports positive selection in cyanobacteria

The phytochrome gene from Microcystis aeruginosa FACHB-912,isolated from algal blooms in Taihu Lake in China,was sequenced and found to code for a wavelength transition light receptor protein.The gene was composed of five distinct domains:a PAS fold domain,a GAF domain,a phytochrome domain,a His Kinase A(phosphoacceptor) domain,and a histidine kinase domain.Red-absorbing phytochrome,far-red-absorbing phytochrome,and photoconversion kinetics were monitored via the spectral characteristics of the protein.To detect positive selection,homologous sequences from cyanobacterium phytochrome genes were obtained from GenBank.Potential selective pressure was identified by phylogenetic analysis with maximum likelihood analyses.The frequency of the phytochrome gene being subjected to selective constraints suggests that positive selection is a potentially important mechanism that promotes the evolution of the cyanobacteria phytochrome gene.The sites identified in this study provide targets for further research on the structural-functional role of these residues,and on the correlation with the mechanism of algal blooms.     

Phytochrome controlled, long-day photoperiod-inducible protein in rice leaves

A new protein in the leaves of NK58S and NK58 (Oryza sativa L. subsp.japonica), which can be induced by 10 d-long-day photoperiod (14 h lightid) and cannot be induced by 10 d-short-day photoperiod (10 h light/d), has been found by two-dimensional gel electrophoresis. The protein, whose molecular weight and isoelectric point are 36 ku and pH 5.2 respectively, is found to be controlled by phytochrome as shown by the experiment of red light induction-far red light reversion.The existence of this protein in both NK58S and NK58 reflects that some of the responses of NK58S and NK58 might be similar in response to long-day photoperiod, a mild stress.     

蓝藻水华显著影响因子识别模型

根据蓝藻水华反演图判断蓝藻水华发生与否,进而构建了直接以蓝藻水华发生与否的指标为因变量,同时以水质、水文和气象3类共19个指标为自变量的蓝藻水华环境影响因子识别模型(Probit模型),并将该模型应用到太湖.经模型分析,识别出太湖蓝藻水华暴发的显著环境影响因子:气温和硝酸盐浓度与蓝藻水华暴发的概率正相关;风速、湿度和电导率则与水华暴发的概率负相关.将离散因变量模型(二元选择模型)引入蓝藻水华研究领域,对湖泊(水库)蓝藻水华暴发的预测和预防具有参考价值.     

云南滇池马村湾、海东湾底栖动物本底调查研究

 报道了2000年4～8月昆明滇池蓝藻水华污染控制技术中,非封闭围隔水体试验前对试验区马村湾、海东湾进行的底栖动物本底调查研究结果,调查中共采到底栖动物15科,40种,其中4种为滇池新记录.环节动物4科,9属,11种;软体动物5科,8属,17种,(其中11种是空壳,活体标本仅6种);节肢动物6科,9属,9种.定量采样点底栖动物平均密度为11089.78个/m²,生物量为58.95g/m².并分析了种类组成、分布与生境的关系,为该项目提供了基础资料.     

群体感应介导的溶藻行为对蓝藻水华控制的研究

水体富营养化加剧了蓝藻水华的暴发,生物方法控藻在有效应对蓝藻水华方面具有广阔的前景.在暴发蓝藻水华的水体中存在大量溶藻细菌,其溶藻能力与群体感应作用紧密联系.溶藻细菌通过群体感应调控胞外溶藻物质的分泌,从而实现对蓝藻水华的控制.通过对溶藻菌群体感应系统、蓝藻自身的群体感应及菌藻关系研究现状的综合分析,探讨溶藻细菌群体感应在蓝藻水华治理中的作用,为蓝藻水华的防控提供参考.     

淡水水体中的蓝藻毒素研究进展（综述）

概述了蓝藻毒素的结构,毒性机理以及蓝藻毒素的危害,并简要介绍了蓝藻毒素的环境行为,对蓝藻毒素的危险评价和监测。     

蓝藻系统的热动平衡及其效应

 将多元复相系的热动平衡条件推广到开放系统,证明可用热动平衡下的流动平衡态来描述生命系统中的物质流动.应用于蓝藻系统,说明水华蓝藻生消的一些问题.     

微囊藻毒素的提取纯化方法比较

研究了从天然蓝藻水华藻粉中提微囊藻毒素,以及对提取物纯化的方法.通过对比不同提取剂的提取效果,发现浓度为80%的甲醇溶液提取效率最高,但采用乙醇作为替代提取剂也有较好的效率,且方法更为安全.对于提取物的纯化,可通过调节溶剂的pH至等电点以除去对反相填料具有负作用的藻胆蛋白.研究结果为更高效地纯化微囊藻毒素提供了依据.