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A circular DNA molecule, designated as DNAβ, was identified in tobacco plants infected with Tobacco leaf curl virus (TLCV) isolates Y5 and Y8 by PCR using primers based on the conserved region of the two reported DNAβ sequences of whitefly-transmitted
geminiviruses (WTGs). The complete nucleotide sequences of DNAβ of Y5 and Y8 (TLCV DNAβ) were determined. Y5 DNAβ comprises
1333 nucleotides encoding 8 predicted ORFs with 4 ORFs in virion-sense DNA and 4 ORFs in complementary-sense DNA; Y8 DNAβ
consists of 1338 nucleotides encoding 7 predicted ORFs with 4 ORFs in virion-sense DNA and 3 ORFs in complementary-sense DNA.
TLCV DNAβ has little sequence homology to DNA-A of TLCV., except that it shares conserved TAATATTAC loop sequence with TLCV
DNA-A. Sequence comparison showed that Y5 DNAβ shared 85% sequence homology with Y8 DNAβ, and both Y5 DNAβ and Y8 DNAβ had
relatively low sequence identity (51%–65%) with the reported DNAβ molecules associated with Ageratum yellow vein virus and Cotton leaf curl virus. The immunotrapping PCR and whitefly transmission tests showed that DNAβ molecule could be encapsidated in virus particle
and transmitted by Bemisia tabaci. This is the first report of DNAβ associated with WTGs in China. 相似文献
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CUIXiaofeng ZHOUXueping 《科学通报(英文版)》2004,49(24):2607-2612
Tomato yellow leaf curl China virus Y10 isolate (TYLCCNV-Y10) alone could systemically infect host plants such as Nicotiana benthamiana without symptoms. In contrast, Tobacco curly shoot virus Y35 isolate (TbCSV-Y35) alone induces leaf curl symptoms in N. benthamiana. When inoculated into transgenic N. benthamiana plants expressing GFP gene (line 16c), TYLCCNV-Y10 neither reverses the established GFP silencing nor blocks the onset of GFP silencing. In contrast, TbCSV-Y35 can partially reverse the established GFP silencing and block the onset of GFP silencing in new leaves. In the patch co-infiltration assays, the AC2 and AC4 proteins of TYLCCNV-Y10 and TbCSV-Y35 could suppress local GFP silencing and delay systemic GFP silencing, suggesting that they are suppressors of RNA silencing. Comparison of the accumulation levels of GFP mRNA in the co-infiltration patches showed that Y10 AC2 and Y35 AC2 proteins had similar efficiency for suppression of RNA silencing. However, Y35 AC4 protein functioned as a stronger suppressor of RNA silencing than Y10 AC4 protein. There-fore, the pathogenicity difference between TbCSV-Y35 and TYLCCNV-Y10 may be related to the functional difference in their AC4 proteins. 相似文献
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陈育庭 《系统工程理论与实践》2002,22(4):109-114
记忆一直作为心理学的核心内容之一而受到极大的重视 .本文从学习记忆心理角度 ,在综合了由观察和实验研究所获得的有关保持与遗忘方面的研究成果的基础上 ,根据赫尔学习系统的基本公设理论 ,运用现代科学中的模型方法 ,构建关于学习记忆心理过程的数学模型 ,并通过齐次平衡法、数值模拟等数学演绎 ,分析解释记忆恢复过程多峰现象 ,提出学习记忆恢复过程的混沌模式。 相似文献
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提出了有关散度算子和旋度算子的见解,简明论述了在矢量分析中的散度和旋度都可用算子形式直接表示,不用首先点积和叉积. 相似文献
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以几何代数多维统一运算为基础, 探讨了多维向量场的多重向量表达与基本运算, 给出了向量场微分的模板卷积计算方法. 利用几何积对内外积的统一表达, 建立散度和旋度的统一计算方法并进行算法实现. 该方法不仅再现了向量场散度和旋度参数的几何关联性及其微分特性, 更实现了多维统一与坐标无关的计算. 基于北美风场的模拟实验显示, 该算法比基于梯度的间接求解算法具有更好的对比度与平滑性特征, 且具有较强的抗噪音能力, 从而可为向量场数据分析提供新的思路与方法基础. 相似文献
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Tomato yetlow leaf curl viruses betong to Begomoviruses of geminiviruses. In this work, we first found and demonstrated that
the small circular DNA molecules were derived from Chinese tomato yetlow leaf curl viruses (TYLCV-CHI). These small circular
DNA molecules are about 1,3 kb, which are half the full-length of TYLCV-CHI DNA A. It was shown by sequence determination
and analysis that there was unknown-origin sequence insertion in the middle of the small molecules. These sequences of unknown-origin
were neither homologous to DNA A nor to DNA B, and were formed by recombination of virus DNA and plant DNA. Although various
defective molecules contained different unknown-origin sequence insertion, all the molecules contained the intergenic region
and part of the AC1 (Rep) gene. But they did not contain full ORF. 相似文献
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针对高速公路弯道处事故率高的特点,研究了一种基于特征点提取的高速公路弯道识别方法,以降低弯道处交通事故发生率。采用Hough变换求出车道线直线方程,根据高速公路的特点建立适当的道路模型,确定对应直线段上的最低点和最高点;分别比较直线段两侧白色像素点的个数判断出曲线道路的弯曲方向,从而进一步确定曲线段上的特征点,同时对车道线候选特征点进行滤波;分段拟合车道线的直线段和曲线段,实现车道线的二维重建。实验表明,能识别不同形状的车道线,具有很高的实时性和准确性,且在不同道路干扰下对连续型分道线和虚线型分道线的检测都有良好的识别效果。 相似文献