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1.
The medical use of bee venom for rheumatoid arthritis ( RA ) has a very long tradition. In this study, isolation and purification of polypeptides from bee venom were carried out on sephadex chromatography, heparin sepharose CL-6B chromatography and HPLC. Several fractions were extracted, and their effects on activation of splenocyte and THP-1 cell were studied. The inhibitory fraction was selected for further studies. Finally, BVⅠ-2H that the HPLC elution profiles was a single peak was isolated by C8 column. ESI- MS detection results showed that BVⅠ-2H was a fraction of bee venom, and the molecular weight of the major component was 644.8. BVⅠ-2H could inhibit ConA-induced splenocyte proliferation, IL-1 production and interfere with splenocyte cycle in mice. Moreover, BVⅠ-2H could inhibit PMA-induced TNFα production in THP-1 cells, which was due to its inhibitory effects on TNFα mRNA expression and protein phosphorylation of IκBα. Our studies indicated that BVⅠ-2H was one of the anti-inflammatory components of bee venom. 相似文献
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The medical use of bee venom for rheumatoid arthritis (RA) has a very long tradition. In this study, isolation and purification of polypeptides from bee venom were carried out on sephadex chromatography, heparin sepharose CL-6B chromatography and HPLC. Several fractions were extracted, and their effects on activation of splenocyte and THP-1 cell were studied. The inhibitory fraction was selected for further studies. Finally, BVⅠ-2H that the HPLC elution profiles was a single peak was isolated by C8 column. ESI- MS detection results showed that BVⅠ-2H was a fraction of bee venom, and the molecular weight of the major component was 644.8. BVⅠ-2H could inhibit ConA-induced splenocyte proliferation, IL-1 production and interfere with splenocyte cycle in mice. Moreover, BVⅠ-2H could inhibit PMA-induced TNFa production in THP-1 cells, which was due to its inhibitory effects on TNFa mRNA expression and protein phosphorylation of IkBa. Our studies indicated that BVⅠ-2H was one of the anti-inflammatory components of bee venom. 相似文献
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分别合成了两种侧链不同的聚肽热致液晶,PDLG和AzoPDLG.两者的主链均为刚直棒状的介晶基元,但PDLG的侧链为柔软的长烷基链,AzoPDLG的侧链则含有刚性的偶氮苯介晶基元和长链的间隔基.利用1H NMR、示差扫描量热分析和偏光显微镜对这些化合物的性质进行了分析与表征,并探讨了不同化学结构的侧链对热致液晶相变行为... 相似文献
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研究了文蛤多肽对体外培养的人肝癌SMMC-7721细胞的抑制作用,结果表明,经5.0μg/mL文蛤多肽处理的体外培养的人肝癌细胞(SMMC-7721)生长缓慢,倍增时间延长,细胞生长抑制率达89.4%;处理后的癌细胞形态发生明显改变,处于G0/G1期的细胞明显增多,而S期和M期细胞减少,出现明显的凋亡峰,凋亡率为22.3%.实验结果表明,文蛤多肽能有效地抑制体外培养肝癌细胞的增殖活动,可通过改变肝癌细胞的形态及细胞周期而明显抑制细胞的增殖. 相似文献
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窦正远 《华南理工大学学报(自然科学版)》1987,(2)
目前采用次氯骏盐漂白纸浆纤维的损伤大,废水严重污染.研究采用无污染漂白剂H_2O_2探索了总碱、碱比、预处理和漂温等对漂白过程的影响和作用机理,指出H_2SO_4预处理后的蔗渣化学浆,在NaOH用量1.5%、Na_2SiO_3用量3.5%、MgSO_4用量0.05%、70℃的漂温下漂白2小时H_2O_2消耗2.367%,经草酸适当的中和还原后,漂浆的白度从39.5%(SBD)提高到7l%,白度增值高,白度的稳定性好,得率也高,因之H_2O_2漂白剂是发展我国草浆漂白有前途的力法. 相似文献
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合成了新型类芪生色团,并通过酰氯化反应制得含芪生色团的单体,将所合成的单体与丙烯酸酯类单体进行自由基共聚制得以类芪生色团为侧基的聚合物.利用核磁共振波谱(^1HNMR)、红外光谱(FTIR)和紫外吸收光谱(UV-Vis),对所合成的单体及聚合物进行了结构表征,并探讨了所制备聚合物的全光开关效应.研究结果表明:在激发光的照射下,通过聚合物中类芪基团的反-顺-反异构循环,在聚合物材料中可形成光致各向异性;所制得的聚合物在较低的驱动光功率(8mW)下具有良好的全光开关效应. 相似文献
7.
含偶氮苯生色团聚乙烯咔唑的全光光开关效应 总被引:2,自引:0,他引:2
应用物理掺杂方法成功制得了以聚乙烯咔唑为主体材料,以偶氮苯生色团染料分散红1、分散红13和分散黄7(DR-1、DR-13、DY-7)为客体材料的全光光开关器件。通过光响应测试发现其在低功率光强(12.5~30mw,514nm)的激发光驱动下具有优良的全光光开关效应。同时从激发光调制频率、生色团含量和染料分子结构等方面进行了比较分析,发现同等实验条件下开关效应随着染料分子极性增大而增强,而生色团掺杂含量较高的(20%)薄膜全光光开关效应较优,较低的调制频率(30Hz)下激发的开关效应优于50Hz下的效应。 相似文献
8.
研究了2,3-二氯-1,4-苯醌和二硫代草酰胺的反应,合成了三个具有平面型结构的含硫萘醌杂环化合物,该类杂环化物可以作为分子内电荷转移复合物的给体或是其它有机电导和有机光导材料的合成原料,所合成的化合物,经过熔点测定,元素分析和红外光谱分析确证了它们的结构。此外,本文还探讨了该类化合物的合成机理和影响合成反应的条件。 相似文献
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小分子多肽合成方法的改进 总被引:3,自引:0,他引:3
采用三苯氯甲烷替代三苯甲醇 ,以 BF3· Et2 O作为催化剂保护巯基 ,产率明显提高(72 %~ 95% ) ;采用乙腈的水溶液作为反应体系 ,控制投料温度 ,活泼酯与氨基酸能顺利结合 ,产率普遍达到 85% ,节省了原料 ,并大大简化了实验操作 .利用上述改进方法成功地合成了有潜在药用前景的新手性小分子多肽 ,其结构得到了严格的谱学鉴定 ,经过了 IR,!HNMR,1 3CNMR,MS或元素分析的确证 . 相似文献