双孢蘑菇酵母菌病害药剂试验

假丝酵母菌(Candida sp．)、酵母菌(Saccharomyces sp．)是稻田栽培双孢蘑菇(Agaricus bisporus)中的一类新病原菌。描述了病原菌的形态特征、致病症状，作了实验室药敏试验。试验表明：克霉灵、灭菌威、甲醛等药剂可有效杀灭病原菌。     

抗生素筛选模型的建立

为节省筛选抗生素的工作量，减少重复筛选率，本实验以常规筛选模型中的指示菌为出发株，经物理化学诱变，筛选出七支突变株，它们对临床上最常用的β内酰胺类、四环素类、氨基糖醇类，大环内酯类和多烯大环内酯类抗生素敏感性显著不同，可构成一抗生素筛选模型。经检验证明通过这一模型能初步判别微生物所产抗生素的类群。     

白色念珠菌基因分型方法研究进展

目的：探讨近年来白色念珠菌基因分型方法研究的新进展。方法：查阅相关文献资料，归纳总结。结果：白色念珠菌基因分型是近年来白色念珠菌分子生物学研究中的一个热点和主要方向，其方法很多。常用的分子生物学技术各有千秋，目前倾向于计算机辅助分析，多种分型技术合用。结论：白色念珠菌基因分型方法研究有新进展，对临床念珠菌病的诊断、治疗及分子流行病学研究等具有重要意义，同时，理想的分型技术还有待进一步研究。     

反胶束中脂肪酶CSL催化性能的研究

以葵花籽油为底物，考察了未见报道的国产脂肪酶ＣＳＬ在ＡＯＴ／异辛烷反胶束体系中的催化性能，当底物体积分数为１０％，ＰＨ＝８．０，Ｔ＝３１３Ｋ，水与表活性剂的浓度比Ｗ０＝１２时，脂肪酶的催化活力最高；而且，当底物体积分数为２０％时，仍未发生抑制现象。     

白色六索线虫一新亚种——中华亚种Hexamermis albicans sinensis ssp.nov.

本文记述了分布于我国安徽麦套棉田以及湖北、河南麦田中,寄生于小地老虎、粘虫幼虫体内的一种六索线虫,这种线虫与世界广布种白色六索线虫的基本特征相似,但又存在着某些地理上的差异,故将之定命为白色六索线虫中华亚种。     

一株粗状假丝酵母(Candida valida)产脂肪酶条件的优化

对粗状假丝酵母(Candida valida)产胞外脂肪酶的培养基成分和培养条件进行了优化研究.结果表明,最佳培养基组成:10 g/L葡萄糖 20 g/L橄榄油,6 g/L酵母膏 4 g/L蛋白胨,K2HPO4:2 g/L,MgSO4:2 g/L,初始pH值为7.5,培养温度为30℃,装液量为30 mL/250 mL三角瓶,接种量为1%,180 r/min摇床培养48 h,培养液酶活高达16.36 U/mL.     

Role of Ppt1 in multiple stress responses in Candida albicans

To study the function of CaPptl, we deleted PPT1 gene from the Candida albicans genome by sequentially replacing the entire coding region with the selectable markers ARG4 and HIS1. The results showed that the deletion of Pptl did not affect the hyphal formation of C. albicans under serum induction and caused enhanced sensitivity to DNA damage, Calcofluor white and salt- induced stress. We also found that Pptl was not required for the phenotypic response of cells treated with the genotoxins, methylmethane sulfonate and hydroxyurea. Flow cytometric analyses indicated that pptlA cells and wild-type cells showed similar G2/M arrest profiles when exposed to DNA damage stress. Pptl was not required for the activation of the DNA damage response pathway, as indicated by normal phosphorylation of Rad53 and Rfa2 in pptlA cells under DNA damage stress. We suggest that Pptl plays important roles in response to various stress conditions in C. albicans.     

粉花羊蹄甲的营养成分及作为特色蔬菜的评价

 粉花羊蹄甲(Bauhinia variegata var.candida Linn)是分布于热带亚洲的苏木科植物,该植物的花是云南热带、亚热带地区许多少数民族经常食用的野生花卉.作者报道了粉花羊蹄甲1981～2002年物侯观测结果,分析了粉花羊蹄甲的矿物质、微量元素、蛋白质、氨基酸和维生素的含量并与常用花卉类蔬菜黄花菜进行了比较.分析结果表明,粉花羊蹄甲花中的矿物质微量元素P,K,Na,Ca,Mg,Fe,Cu和Mn的含量均高于黄花菜,Zn的含量低于黄花菜.维生素中,粉花羊蹄甲维生素B1高于黄花菜,维生素C和胡萝卜素的含量分别是黄花菜的6.4倍和4.5倍,维生素B2和维生素E的含量略低于黄花菜.粉花羊蹄甲的蛋白质总质量分数(15.79%)低于黄花菜(19.19%),但蛋白质中氨基酸总质量分数为12.74%,高于黄花菜(9.99%);比较粉花羊蹄甲和黄花菜蛋白质中必需氨基酸与标准蛋白质(鸡蛋)中必需氨基酸贴近值,粉花羊蹄甲的贴近值为0.85,比黄花菜的贴近值(0.73)高.认为粉花羊蹄甲是一种营养丰富、具有很高开发潜力的野生食用花卉.     

念珠菌病的临床特点

综合有关资料及临床实践对白色念珠菌病的发病机理,病因及诊断作一概述     

Heat-shock protein 90 inCandida albicans

Researches on Candidal heat-shock protein 90 (HSP90) in recent years are summarized.Candida albicans is a commensal pathogen in human and animals. In immunocompromised individuals it behaves as an opportunist pathogen, giving rise to superficial or systemic infections. Systemic candidosis is a common cause of death among immunocompromised and debilitated patients, in which the mortality is as high as 70%. HSP90 is now recognized as an immunodominant antigen inC. albicans and plays a key role in systemic candidosis as a molecular chaperone. The 47-ku peptide is the breakdown product of HSP90. Patients who has recovered from systemic candidosis produce high titre of antibodies to 47-ku antigen, whereas the fatal cases have little antibody or falling titres. The three commonest epitopes of candidal HSP90 have been mapped, epitopes C, B and H. Epitopes C and H are immunogenic. The antibody probes of both epitopes may be developed into a new serological test agents for systemic candidosis due to rather high specificity and sensitivity. The recent results establish HSP90 as an ATP-dependent chaperone that is involved in the folding of cell regulatory proteins and in the refolding of stress-denatured polypeptides. Some researches on fungal HSP90 and the treatment of patients with candidosis are reviewed as well.