公理化局部有限空间中映射连续性的扩展

用公理化方法研究了局部有限空间中的连续映射及其扩张问题。给出了局部有限空间的公理化定义方法;利用邻近关系研究了局部有限空间中的连续映射、同胚和局部同胚等问题;通过对局部有限空间变形的研究,定义了局部有限空间的一种特殊收缩核,有效地解决了局部有限空间中连续映射的扩张问题。     

大鼠血管内皮细胞siRNA转染条件的优化

目的:旨在优化siRNA转染大鼠血管内皮细胞的转染条件.方法:将0.75μL和1.5μL的脂质体LipofectamineTM3000分别与20、40、60、80nmol带荧光标记的FAMsiRNA组合,转染6、12、18、24h后,用荧光显微镜计数阳性细胞率、MTT法检测各浓度条件下内皮细胞的存活率,筛选最优转染条件.结果:(1)转染12h后,用荧光显微镜检测20、40、60、80nmol各组,均可观察到绿色荧光(2)siRNA浓度为60nmol/L,脂质体为1.5μL的组合转染效率最高,继续增加siRNA的浓度,转染效率提高不明显.(3)转染时间超过24h,各组细胞荧光减弱,细胞死亡率显著增加.结论:结果表明,以1.5μL LipofectamineTM3000与60nmol/L的siRNA浓度组成转染混合物转染12h可以实现对大鼠血管内皮细胞高效转染并保持较高的细胞活性.     

中国大气颗粒物污染与致癌研究进展

 室外空气污染和污染空气中的颗粒物是一类致癌物，而近年中国的大气颗粒物污染仍然维持在较高浓度水平。通过综述中国近年大气颗粒物污染现状和开展的颗粒物致癌机制研究，分析中国研究中颗粒物有关实验动物致癌性的证据和颗粒物致癌的人群流行病学线索，阐述大气颗粒物与癌症的关系，提出中国亟需开展相关研究。     

哺乳动物核移植研究进展

核移植技术具有重大的生物学的意义，特别是体细胞核移植技术的成功表明动物分化的体细胞核可再程序化，哺乳动物核移植的研究虽然还存在许多问题需要解决，但在挽救濒危动物，转基因动物研究，人类器官移植等许多方面展现了广阔的应用前景．     

光电转换体系太阳能聚集器

In standard P-V systems, the high cost and low quantum efficiency of silicon cells has held back widespread adoption of this technology for decades. To generate electrical power from solar energy in a cost effective way, most methods require the concentration of the sunlight in order to increase the efficiency of energy conversion. Most systems use parabolic mirrors to focus sunlight to either a line （trough systems） or a small volume （dishes）. One of the reasons solar power still remains little utilised, is the high cost of the collection systems. Light focussing devices using reflection usually take the form of a curved rein＇or. In contrast, this paper describes the new design of a device consisting of an array of small angled reflecting m/trot facets located in a planar form. The three dimensional angle of each facet in the array is a function of its position in the array, and is calculated to be such that for a parallel beam striking the array, each facet will reflect the light in such a way as to form a focal point region.     

Science Letters： A synthetic Toll-like receptor 2 ligand decreases allergic immune responses in a mouse rhinitis model sensitized to mite allergen

It has been proposed that activation of Toll-like receptors （TLRs） plays crucial roles in the polarization of adaptive immune responses. A synthetic Toll-like receptor 2 （TLR2） ligand, Pam3CSK4, has been reported to modulate the balance of Th1/Th2 responses. We evaluated the modulation effect of Pam3CSK4 on allergic immune response in a mouse rhinitis model sensitized to house dust mite allergen （HDM）. Mice were sensitized and challenged with Dermatophagoidesfarinae allergen （Der f）, and then the allergic mice were treated by Pam3CSK4. Nasal allergic symptoms and eosinophils were scored. Der f-specific cytokine responses were examined in the splenocytes and bronchoalveolar lavage fluid （BALF）. Serum level of total IgE was also detected. After establishing a mouse allergic rhinitis model with HDM, we have showed that Pam3CSK4 treatment not only ameliorated the nasal allergic symptoms remarkably but also decreased the eosinophils and total inflammation cells in BALF significantly. Analysis of cytokine profile found that IFN-7 released from either BALF or stimulated splenocytes increased markedly in Pam3CSK4-treated mice, while IL-13 decreased significantly. Moreover, serum level of total IgE was significantly lower in Pam3CSK4-treated mice than in the untreated. Thus, in an allergic rhinitis mouse model developed with HDM, Pam3CSK4 was shown to exhibit an antiallergic effect, indicating its potential application in allergic diseases.     

（BN）n及SiB（n-1）Nn（n=2-11）稳定结构的研究

利用遗传算法及Gastreich提出的经验势函数,研究了（BN）n（n=2-11）和SiBn-1Nn（n=2-11）团簇的可能稳定结构,对能量较低的异构体在HF/STO-3G水平进行优化,得到了（BN）n（n=2-11）和SiBn-1Nn（n=2-11）团簇稳定结构单环及线状结构,并进一步分析了单环的结构特征及相对稳定性。     

Eph受体家族和Ephrin-A5在NSCLCs中上调表达及其临床意义

采用荧光实时定量PCR的方法在48个非小细胞肺癌组织以及配对的正常肺组织中检测Eph/ephrin 基因的mRNA水平.发现肺癌组织中EphB4, EphB3 和 ephrin-A5的表达水平与配对的正常肺组织相比分别上调了60%,81%和65%,并具有显著的统计学差异. 而且,统计学分析发现ephrin-A5的表达水平与肿瘤分型、转移、性别、吸烟以及诊断年龄等临床病理学参数之间存在显著的相关性.此外,EphA2的表达水平与肿瘤大小、家族遗传史以及诊断年龄之间也存在显著的相关性.总之,这些结果提示EphB4, EphA2, EphB3 和ephrin-A5 在肺癌的发生和发展过程中可能以一种单独或者协同的形式发挥作用.这些蛋白有可能成为研究和诊断肺癌的重要靶向基因.     

局部复发性直肠癌的再手术治疗

探讨局部复发性直肠癌(直肠癌根治术后)再次手术的价值、方法、回顾性分析再手术治疗的局部复发性直肠癌33例.结果:21例行根治术,12例行姑息性手术.根治术组和姑息性手术组中位生存期分别为35.2个月(8～82个月)、13个月(2～23个月),再手术5年生存率为34.8%.结论:对于局部复发性直肠癌仍应积极手术,有助于延长生存期,提高生存质量.