Fragmentation study of two brassinolides by ion trap tandem mass spectrometry

The fragmentation pathways of two isomers of brassinosteroids, 28-homobrassinolide (28-h-BL) and 28-epihomobrassinolide (28-eh-BL), have been investigated by tandem mass spectrometry (MSⁿ, n = 1, 2, 3, 4) with the electrospray ionization (ESI) source. The ESI mass spectrometric fragmentation pathways of protonated 28-h-BL and 28-eh-BL were comprehensively elucidated, and the principles revealed in this investigation could potentially be used to identify and distinguish brassinosteroids and their isomers.     

激素对甘蓝型油菜(Brassica napus L)矮化突变体幼苗生长及内源GA3含量的影响

以甘蓝型油菜矮化突变体“DDF-1”为材料,研究了赤霉素（GA3）、生长素（IAA）和油菜素内酯（BR）3种激素对突变体幼苗的影响,并用石蜡切片观察其细胞形态,用酶联免疫吸附技术(ELISA) 检测下胚轴内源激素GA3含量.结果表明,外加一定浓度的GA3（7 mg/L）对矮化突变体“DDF-1”的下胚轴伸长作用显著,但不能使之恢复到野生型的长度;萌发早期,BR有明显的促进伸长作用,萌发后期效果不明显;突变体对IAA敏感性较弱.三种激素交互作用对矮秆下胚轴伸长的影响也不显著.施加外源GA3的矮化突变体“DD     

油菜素甾体类化合物的生物活性及其在农业生产中的应用

阐述了油菜素甾体类化合物的生物活性与已知植物激素的关系，以及在农业生产中的应用。