浅谈随刊附盘的著录与管理

介绍了随刊附盘的种类及其验收与著录的方法,提出了随刊附盘管理和利用的措施。     

Cloning, construction of prokaryotic expression vector and expression ofEscherichia coli cytosine deaminase gene

Cytosine deaminase gene ofEscherichia coli strain H-30 was cloned, and its initiation codon of ‘GTG’ was mutated to ‘ATG’ by PCR. Prokaryotic recombinant expression vector pBV220-CD was constructed. Clone with high enzyme activity were selected by detecting their specific activity of cytosine deaminase. 5-FC(5-FC, 5-fluorocytosine) could induce the lethal toxicity to cells containing active CD gene. DNA sequence analysis indicated that there were 16 altered bases and 5 of them resulted in the alteration of amino acids in predicted peptide by comparing DNA sequence of the clone H-30-CD-11 with high enzyme activity with CD gene reported in Gene Bank.     

Two tyrosines in CD3ε-ITAM are required to induce T lymphocyte apoptosis

CD3ε of T cell antigen receptor complex (TCR/CD3) plays an important role in the resembling of the complex and activation signaling through its conservative immunoreceptor tyrosine-based activation motif (ITAM) in the cytoplasmic tail. Previous study showed that a chimera molecule, consisting of the extracellular-transmembrane domain of human CD8α fused to the cytoplasmic domain of CD3ε, induced apoptosis of T lymphocytes, indicating that apoptotic signals were transduced through the CD3ε- ITAM. To elineate involvement of the two tyrosines in apoptotic signaling pathway, cDNAs with mutations at Y170F, Y181F and Y170F/Y181F in CD8-ε-ITAM were made by point mutation and PCR, and then cloned into pcDNA3 eukaryotic expression vectors. Stable expression cell lines were established after transfection of the expression vectors into CD8+- Jurkat T lymphocytes. Stimulation of these cell lines with anti-CD8 monoclonal antibody showed that only the cells with expression of wild type chimera CD8-ε died by apoptosis, but not those cells with expressions of mutated CD8-ε chimera, indicating that the two tyrosines in CD3ε-ITAM were required for the apoptotic signal transduction in T lymphocytes.     

以太网建设中的关键技术问题

以太网拥有最大广泛的用户,以太网新技术的不断出现又给传统以太网注入了活力。深入理解以太网技术,对改造原有以太网和构造新网都有重要意义,本文论述以太网建设中的重要技术问题及建网实务。     

有机手性分子器件的光电特性

目前多种有机手性分子器件虽已研制成功,但其内在的物理机理仍有许多未解之谜,其中手性分子的圆二色性精细结构、手性诱导的自旋选择效应等是有机手性分子研究的核心.为设计基于手性的新型有机功能器件提供理论指导,考虑有机手性分子螺旋势场诱导的自旋-轨道耦合,围绕分子的光电特性及其调控展开了系列研究.根据光致跃迁理论,得到手性分子...     

α-胰凝乳蛋白酶在不同介质中稳定性的研究

测定了α-胰凝乳蛋白酶在不同介质中的活性及稳定性。研究了有机溶剂性质、pH值、离子强度及离子类型对α-胰凝乳蛋白酶活性与稳定性的影响,并通过CD谱初步研究了α-胰凝乳蛋白酶在不同pH值缓冲液中的二级结构变化。研究结果表明,α-胰凝乳蛋白酶在某些有机溶剂中能在较长时间内维持较高的酶活性。α-胰凝乳蛋白酶在3种pH值不同的缓冲液中的CD谱表明其在pH＝10.0的稀溶液中的α-螺旋和（或）β-折叠含量较高。在不同pH值条件下,离子强度对α-胰凝乳蛋白酶的活性与稳定性的影响不同。     

重载下CSMA/CD总线性能分析

本文基于分时间片非坚持型CSMA/CD协议,提出了一种新的适合于重负载下评价CSMA/CD总线性能的数学模型.该模型合理地考虑了重载下各种新现象及影响总线性能的诸因素.实验研究结果表明,本文提出的模型较以前的评价模型更接近于实际系统.     

DNA的CD谱和ORD谱的变换

本文介绍了用ＫｒｏｎｉｇＫｒａｍｅｒｓ变换公式将ＤＮＡ的ＣＤ谱变换成ＯＲＤ谱的方法，并将实验结果作了变换计算     

基于总线以太网的控制协议改进

为了解决实时控制网络应用中的通讯实时性和确定性问题,提出在总线以太网上实现一种CSMA/CD-Master/Slave切换混合协议,并在其上构建UDP/IP协议,同时对该混合协议的实时性和可靠性进行计算机仿真分析.在实时控制网络应用中,当网络轻载时,采用CSMA/CD访问控制方式;当网络重载时,自动切换到Master/Slave方式,实现自动调节达到对介质的有限争用.仿真表明CSMA/CD-Master/Slave切换混合协议的应用能够提高通讯实时性和可靠性,可以保证控制网络的稳定性和实效性.     

HCV感染通过Erk信号的激活上调PTTG1的表达

 为探讨丙型肝炎病毒(HCV)感染导致肝细胞癌发生的分子机制,利用前期研究建立的体外HCV细胞培养体系,将HCV JFH-1 RNA转染CD81-Huh7细胞,确定能够获得感染性HCV后,收集HCV转染后10,50,100和150d的细胞,采用Real time PCR及Western Blot法检测不同时间点细胞内垂体瘤转化基因1(PTTG1)基因和蛋白水平的表达情况,同时检测总MAPK/Erk1/2,磷酸化Erk1/2(p-Erk1/2)蛋白的表达。随后,用干扰素抑制HCV的感染,再检测PTTG1及MAPK/Erk1/2的表达情况,以及用MAPK/Erk抑制剂(PD98059)阻断MAPK/Erk 1/2的磷酸化后,检测PTTG1的表达情况。结果显示,HCV转染的细胞与未转染细胞比较,PTTG1的mRNA和蛋白表达均显著增加,p-Erk1/2蛋白显著升高(P＜0.05);抑制HCV感染显著降低PTTG1的表达和MAPK/Erk1/2的磷酸化(P＜0.05);MAPK/Erk1/2抑制剂显著降低了PTTG1基因和蛋白的表达(P＜0.05)。体外HCV感染可以导致MAPK/Erk信号通路的磷酸化,进一步导致原癌基因PTTG1的表达增加,这可能是慢性HCV感染导致HCV相关性肝细胞癌发生的分子机制之一。