TRAIL与kallistatin联合表达载体的构建及抗肿瘤活性分析

为研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)与组织激肽释放酶结合蛋白(kallistatin)联合用药的抗肿瘤作用,构建TRAIL与kallistatin双表达的重组质粒pAM-CAG-Kal-IRES-TRAIL,将重组质粒转染A549,LO-2,NCI-H446和Hela细胞,考察其抗肿瘤活性.实验结果表明:构建的双表达载体能同时表达TRAIL与kallistatin,且均能分泌至培养基中;TRAIL与kallistatin联合表达对肿瘤细胞活力的抑制作用明显增强,诱导肿瘤凋亡的作用也明显增强,说明联合表达TRAIL与kallistatin能够增强抗肿瘤活性.     

凋亡抑制蛋白与肿瘤关系的研究

凋亡抑制蛋白(inhibitor of apoptosis proteins,IAPs)可以作为癌症诊断和治疗的靶点,在肿瘤研究方面备受关注,就凋亡抑制蛋白的结构、功能、在肿瘤中的表达与肿瘤的关系等研究进展作一综述,为凋亡抑制蛋白的研究提供信息和思路.     

骨髓干细胞的可塑性及其在栓塞性疾病中的应用

栓塞性血管阻塞在急性心肌梗死、脑梗塞、血栓闭塞性脉管炎、糖尿病足等发病过程中起着重要作用。目前临床上针对这些疾病的治疗手段主要有：药物溶栓、血管旁路移植和介入手术等,多年来其治疗方法无实质性进展。上个世纪九十年代人们发现了干细胞新的生物学特性—可塑性,对干细胞可塑性的研究以及相关技术的发展为心脏病、老年痴呆、帕金森等的治疗带来了希望,也为栓塞性疾病的治疗开辟了新途径,有望成为治疗手段之一。     

肿瘤患者的心理分析及护理

分析了肿瘤患者心理变化4个阶段的不同表现,并针对此提出相应心理护理的方式。指出加强肿瘤患者的心理分析及护理是保证顺利治愈的重要环节。     

Thrombospondin co-localises with TGFβ and IGF-I in the extracellular matrix of human osteoblast-like cells and is modulated by 17β estradiol

Thrombospondin (TSP) is a multifunctional glycoprotein which is synthesised by several cell types including osteoblasts, and incorporated into the extracellular matrix (ECM) of these cells. The function and regulation of TSP in bone is not clear. In this study, using a long term culture model of human osteoblast-like cells, we examined the distribution of TSP in the ECM and its modulation by added estradiol. In this model the osteoblast-like cells form a regular multilayer which continues to increase in depth up to 50 days post confluence. In the ECM of these cultures and in 19-week fetal bone, the bone markers osteocalcin and alkaline phosphatase were diffusely distributed in the matrix. In contrast, labelling for TSP was concentrated, confined to the banded collagen and its immediately adjacent ECM. This pattern of labelling resembled that of the growth factors transforming growth factor-I (TGF), and insulin-like growth factor-I (IGF-I), with which TSP label co-localised. Labelling intensities were comparable between fetal bone and the in vitro material for TSP, TGF and IGF-I. TSP label was present by 10 days post confluence, reached a maximum by 20 days, and declined slowly thereafter, a time course which was similar to that of IGF-I. Incubation of osteoblast-like cell cultures with 17 estradiol resulted in an increase in multilayer depth and a maximal 3-fold increase in TSP labeling at 30 days as well as approximately 2-fold increases for TGF and IGF-I. The dose-response relationship for these responses to estradiol treatment was biphasic with maximal increases at 10^–10 M–10^–11 M of added estradiol. Treatment with 17 estradiol produced labelling intensities that were not significantly different from controls. Studies with other cell types have suggested that TSP may be involved in modulation of growth factor activity. The similarities between TSP, TGF and IGF-I, in terms of their distribution and regulation by 17 estradiol treatment, may indicate a role for TSP in modulating bone cell proliferation and function through interaction with local growth factors.     

不同贮藏条件下鳗骨油氧化特性的研究

研究了室温下（15℃）鳗骨油在空气中的自氧化速度,以及温度和贮藏条件对鳗骨油氧化速度的影响。实验所设的温度梯度分别为6℃、15℃、35℃,贮藏条件分别是敞口,密封（蜡封）和用N2驱除氧气后密封保存。实验结果显示,室温下鳗骨油在空气中的自氧化速度,敞口存放4天,过氧化值就已超过0.15;温度和贮藏条件对鳗骨油的氧化速度有显著影响,驱除氧气低温贮藏可以有效地防止鳗骨油的氧化。     

鸭血烯酸对艾氏癌生长的抑制作用

鸭血烯酸是由鸭血清中提取的不饱和脂肪酸．在体外实验中具有使艾氏腹水癌细胞膨胀失活的作用．用于治疗艾氏癌患鼠,可抑制其腹水癌和实体瘤的生长．与环磷酰胺（CPA）或氟脲嘧啶（SFU）相比,鸭血烯酸的毒性较低,疗效较高,约20％～40％被治愈的患鼠,可长期存活．     

颅骨弹性模量时效性的实验研究

采用猪颅骨为实验材料，使用应变电测法对颅骨各层弹性模量随风干时间的变化规律进行了实验研究。实验结果表明：密质骨弹性模量约是松质骨弹性模量的2．5倍，松质骨对颅骨弹性模量的影响有限；猪颅骨单层密质骨和整体的弹性模量相差约13．9％；冷冻后经室温下自然解冻的猪颅骨单层密质骨的弹性模量变化量相当于新鲜密质骨的9．2％，单纯板障松质骨片的变化量为17％，去掉一层密质骨后的颅骨片变化量为18．1％，整体猪颅骨片的变化量为15．6％；由于各生物体颅骨结构基本相同。由此可知，如果在人颅骨上也能得到相似的结论，在进行有关人颅骨研究时可大大延长同一块颅骨的使用时间。或者可以用风干颅骨、解冻后风干颅骨来代替新鲜骨，然后根据其时效特性对实验数据进行修正，这样会大大降低实验成本和难度。     

骨髓干细胞与急性心肌梗死的血管新生

骨髓干细胞可分化为心肌细胞、血管内皮细胞、平滑肌细胞等，能再生心肌和血管，可用于心肌梗死的治疗。本文就骨髓干细胞在血管新生方面的生物学特性及其对心梗后的血管新生作用作一综述。