Isolation and purification of BVⅠ-2H from bee venom and analysis of its biological action

The medical use of bee venom for rheumatoid arthritis ( RA ) has a very long tradition. In this study, isolation and purification of polypeptides from bee venom were carried out on sephadex chromatography, heparin sepharose CL-6B chromatography and HPLC. Several fractions were extracted, and their effects on activation of splenocyte and THP-1 cell were studied. The inhibitory fraction was selected for further studies. Finally, BVⅠ-2H that the HPLC elution profiles was a single peak was isolated by C8 column. ESI- MS detection results showed that BVⅠ-2H was a fraction of bee venom, and the molecular weight of the major component was 644.8. BVⅠ-2H could inhibit ConA-induced splenocyte proliferation, IL-1 production and interfere with splenocyte cycle in mice. Moreover, BVⅠ-2H could inhibit PMA-induced TNFα production in THP-1 cells, which was due to its inhibitory effects on TNFα mRNA expression and protein phosphorylation of IκBα. Our studies indicated that BVⅠ-2H was one of the anti-inflammatory components of bee venom.     

基于多目标蜂群算法的数据分类方法

为了保证运算时效的同时,提高复杂数据的分类精度,提出了基于多目标蜂群算法和极限学习机的数据分类算法。该方法以最小的特征个数和最高的分类精度为优化目标,利用改进的多目标蜂群算法对数据的特征个数和分类器参数进行寻优,针对多个有代表性的数据集进行仿真,结果表明所提出方法的有效性。     

人工蜂群算法及其应用

为促进人工蜂群算法理论和应用的发展, 在分析人工蜂群算法的基本原理基础上, 针对算法的不足, 全面地归纳了国内外学者对算法的改进研究, 对算法的蜜源初始化、更新策略的改进、调整策略的改进、适应度函数的选择以及与其他算法的融合进行综述, 提出了更有效的改进策略。同时从多方面综述了人工蜂群算法的应用, 并对人工蜂群算法的发展方向进行了总结和展望。     

数据中心启发式反向人工蜂群虚拟机整合节能策略

针对数据密集型作业的特点,提出一个基于CPU和图形处理器(GPU)两个影响因素构建计算节点的能耗评估模型.该模型基于原虚拟机选择节能算法(ABCS)在虚拟机选择节能策略中的能效优势,进一步利用启发式思想改进蜂群优化算法,寻求虚拟机整合的最优解.在CloudSim 3.0云计算模拟器中的实验结果表明,启发式反向蜂群算法能在保证服务质量的前提下,有效降低虚拟机迁移次数,进而降低数据中心的能耗(节能25%~30%).     

中华蜜蜂工蜂上颚腺的超微结构

对中华蜜蜂工蜂上颚腺超微结构的电镜观察,发现上颚腺呈囊状,胞内管和胞外管的管壁由3层结构组成。细胞连接为隔壁桥粘连接。核为卯圆形,腺细胞内含有许多核糖体和粗面内质网,滑面内质网也存在。线粒体和高尔基复合体也被观察到。     

Isolation and purification of BVⅠ-2H from bee venom and analysis of its biological action

The medical use of bee venom for rheumatoid arthritis (RA) has a very long tradition. In this study, isolation and purification of polypeptides from bee venom were carried out on sephadex chromatography, heparin sepharose CL-6B chromatography and HPLC. Several fractions were extracted, and their effects on activation of splenocyte and THP-1 cell were studied. The inhibitory fraction was selected for further studies. Finally, BVⅠ-2H that the HPLC elution profiles was a single peak was isolated by C8 column. ESI- MS detection results showed that BVⅠ-2H was a fraction of bee venom, and the molecular weight of the major component was 644.8. BVⅠ-2H could inhibit ConA-induced splenocyte proliferation, IL-1 production and interfere with splenocyte cycle in mice. Moreover, BVⅠ-2H could inhibit PMA-induced TNFa production in THP-1 cells, which was due to its inhibitory effects on TNFa mRNA expression and protein phosphorylation of IkBa. Our studies indicated that BVⅠ-2H was one of the anti-inflammatory components of bee venom.     

基于Boltzmann选择的人工蜂群KFCM算法

为提高算法的搜索效率、减少搜索过程中陷入局部最优的现象,将人工蜂群算法用于核模糊C-均值聚类,但在聚类数比较大和维度较高时效果不太好,为此引入Boltzmann选择机制代替轮盘赌的选择方式,并采用小区间生成法使初始群体均匀化,使得该算法的全局寻优能力更强,有效克服了KFCM算法易陷入局部最优的缺点.实验结果表明,对于聚...     

阜阳地区草莓“简、密、蜂”新型栽培模式探讨

研究不同的大棚结构、定植密度及授粉方式对草莓(Fragaria gracilis Losinsk.)产量和品质的影响。结果表明,采用简易半坡式大棚,东西走向,坐北向南可提高光合效率,增强保温性能,使草莓成熟期提前,获得早上市、高产草莓;以12000株/667m2左右的密度定植,待其顶花序果实采收完毕后,及时调整密度,降至6000株/667m2左右,可较大幅度提高草莓产量;采用蜜蜂授粉加人工辅助授粉的方法可获得优质草莓。研究结果为高产优质草莓生产提供了参考。     

基于粒子群与蜂群结合的算法求解含风电场电力系统经济调度问题

提出一种新的混合智能算法解决含阀点效应和系统约束的含风电场的电力系统经济调度问题,将蜂群中的觅食行为与聚群行为引入改进的粒子群,提出改进粒子群一蜂群混合智能算法．在算法上进行优化,大大地提高搜索的能力,从而使结果更优．通过引入交叉策略,对那些速度保持不变的点,重新赋值．以一定的比例选拔最优点,其中选拔出的最优点,不止一个．同时精英策略的采用,有利于加强全局寻优,跳出局部最优,从而使算法得到很大的改善．最后对一个10机系统的算例进行求解,通过与改进的粒子群算法、蜂群算法进行比较,验证了改进的粒子群一蜂群混合智能优化算法在解决含风申．场的申力系统终济调度问题中的有效性与优撼性．     

贝叶斯预测蜂群算法在无线传感器网络优化中的应用

针对无线传感器网络(WSN,wireless sensor network)节点分布不合理,存在较多的监测盲区等不足,提出了利用贝叶斯预测人工蜂群算法(BPABC,Bayesian predictive artificial bee colony algorithm)制定节点分布方案。BPABC算法借鉴贝叶斯预测算法的思想对蜂群算法中各蜜源存在最优解的概率进行预测,并以此为依据指导跟随蜂寻优工作。采用BPABC算法对WSN中的节点分布进行优化,与人工蜂群算法、全局人工蜂群算法制定的优化方案进行比较。结果表明,BPABC在平均覆盖率、最差覆盖率等方面均优于其他两种算法,并且BPABC算法在迭代收敛速度方面也有明显的优势。为了进一步验证改进算法的实用性,采用BPABC制定不同监测区域的WSN节点分布方案。WSN的覆盖率均在97%左右,并且标准差不超过0.005%。由此可见,基于BPABC的WSN节点分布优化方案具有较高的覆盖率、良好的适应性和稳定性。