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DU JinFeng LI Ying ZHANG Qi YAO XiaoJun 《科学通报(英文版)》2007,52(2):200-204
The presence of several high affinity binding sites on human serum albumin (HSA) makes it a possible target for many drugs. This study is designed to examine the effect of aloe-emodin on HSA by fluo-rescence, CD spectroscopy and molecular modeling. The results of fluorescence measurements sug-gested that the hydrophobic interaction was the predominant intermolecular force stabilizing the AE-HSA complex, which was in good agreement with the result of molecular modeling study. And the enthalpy change ΔH0 and the entropy change ΔS0 were calculated to be -7.041 kJ·mol-1 and 76.619 J·mol-1·K-1 according to the Van't Hoff equation. The alterations of protein secondary structure in the presence of AE in aqueous solution were quantitatively calculated from CD spectra, and the content of α-helices obviously increased. 相似文献
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本文提出了制取芦荟各类原汁的方法,并初步证实了芦荟大黄素类、芦荟苦素类和芦荟宁的存在及分布趋势。从而指出:凝胶原汁应用于日化产品将是最主要的开发方向之一。 相似文献
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荧光光度法测定中药芦荟大黄素 总被引:1,自引:0,他引:1
齐登福 《山东理工大学学报:自然科学版》2002,16(2):5-8
利用荧光光度法研究确定了测定芦荟大黄素的适用条件 .在λex/em =470 /5 4 0nm ,pH =7.0的实验条件下 ,测定的线性范围为 1 .0~ 8.1 μg .mL- 1 ,检测限为 0 .0 3 1 μg .mL- 1 ,方法精密度为 2 .1 8% ,并成功地测定了样品决明子、血清和尿样中芦荟大黄素的含量 . 相似文献
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研究芦荟大黄素(AE)在单壁碳纳米管修饰玻碳电极(SWNTs/GCE)上的电化学行为.结果表明SWNTs/GCE对AE具有良好的电催化性能.在9.0×10-9~4.4×10-6 mol· L-1范围内,其差分脉冲伏安法(DPV)峰电流与AE的浓度呈良好的线性关系,检测限为5.0×10-9 mol·L-1,可用于药物制剂... 相似文献
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