一种高效纯化血清淀粉样物质A(SAA)的方法

通过密度梯度超速离心、脱脂从急性相胸水中分离得到含有血清淀粉样物质A（Serum AmyloidA,SAA）的高密度脂蛋白（HDL），再通过两次Sephacry1S-200层析纯化SAA蛋白。经SDS-PAGE电泳和Western Blotting检验证实得到的SAA蛋白纯度高，可作为抗原免疫动物制备抗体，方法可用于从胸水中大量制备SAA。     

Apolipoprotein M affecting lipid metabolism or just catching a ride with lipoproteins in the circulation?

Apolipoprotein M (apoM) is a novel apolipoprotein found mainly in high-density lipoproteins (HDL). Its function is yet to be defined. ApoM (25 kDa) has a typical lipocalin ?-barrel fold and a hydrophobic pocket. Retinoids bind apoM but with low affinity and may not be the natural ligands. ApoM retains its signal peptide, which serves as a hydrophobic anchor to the lipoproteins. This prevents apoM from being lost in the urine. Approximately 5% of HDL carries an apoM molecule. ApoM in plasma (1 μM) correlates strongly with both low-density lipoprotein (LDL) and HDL cholesterol, suggesting a link to cholesterol metabolism. However, in casecontrol studies, apoM levels in patients with coronary heart disease (CHD) and controls were similar, suggesting apoM levels not to affect the risk for CHD in humans. Experiments in transgenic mice suggested apoM to have antiatherogenic properties; possible mechanisms include increased formation of pre-? HDL, enhanced cholesterol mobilization from foam cells, and increased antioxidant properties. Received 28 November 2008; received after revision 15 December 2008; accepted 16 December 2008     

PLI-CRC校验码在EoS封装技术中的实现

EoS( Ethernet over SDH)技术结合了SDH和以太网两者的优势,实现了以太网数据在SDH上传输.针对硬件资源优化及EoS系统中数据帧长PLI的CRC-16校验码算法特点,提出基于FPGA的改进实现方法,通过电路仿真与综合结果表明,该方法实现了对高速并行化数据传输的有效保护,在资源消耗、实现效率两方面都取得了较好效果.     

基于EDA工具的数字电子系统设计

介绍了基于 EDA工具的硬件电子系统的设计方法 ,并针对特定的在系统可编程 CPL D器件 isp L SI10 16目标芯片描述了自动洗衣机模拟控制器的程序设计步骤 ,给出了 HDL程序代码     

最优状态编码研究

探讨了状态机电路最优状态编码的问题。研究了状态编码的成本及寄存器最优、组合逻辑最优之间的关系，介绍了3种常用的编码方法。通过实验分析差异。     

Verilog RTL模型

VLSI集成电路芯片测试技术正在向高层次测试推进，针对Verilog硬件描述语言，提出了一种在寄存器传输级（register transfer level,RTL)上的电路模型VRM，该模型着重于实际应用，可输出文本格式文件，便于开发实用的RTL级故障模拟和RTL级测试生成等软件。基于该模型。还实现了一个简单的RTL逻辑模拟程序以验证VRM模型的可行性。     

Molecular pathogenesis of apolipoprotein E-mediated amyloidosis in late-onset Alzheimer’s disease

Apolipoprotein E (apoE) ɛ4 allele is a genetic risk factor for late-onset familial and sporadic Alzheimer’s disease (AD). In the central nervous system, apoE is secreted mainly by astrocytes as a constituent of high-density lipoproteins. A recent study using apoE knockout mice provided strong evidence that apoE promotes cerebral deposition of amyloid β protein (Aβ). However, no clear explanation of the pathogenesis of apoE-induced AD has been provided. Here we discuss two possible mechanisms by which apoE might enhance Aβ deposition. One is the intracellular pathway in which apoE is internalized by neurons and induces lysosomal accumulation of Aβ and amyloidogenic APP (amyloid precursor protein) fragments, leading to neuronal death. The other is the extracellular pathway in which apoE-containing lipoproteins are trapped by Aβ1–42 deposits mobilizing soluble Aβ peptides and consequently enlarge amyloid plaques. These two mechanisms may operate at different stages of AD pathogenesis and suggest a chaperone-like function for the apoE molecule. Received 4 February 1999; received after revision 9 April 1999; accepted 23 April 1999     

Verilog HDL语言RTL级描述的可综合性

所谓逻辑综合就是将较高抽象层次的描述自动转换到较低层次描述的一种方法。由于Ｖｅｒｉｌｏｇ ＨＤＬ（Ｖｅｒｉｌｏｇ Ｈａｒｄｗａｒｅ Ｄｅｓｃｒｉｐｔｉｏｎ Ｌａｎｇｕａｇｅ）本身的特点，许多面向仿真的语句虽符合语法规则却是不能综合的，这在设计中必须加以避免。同时讨论了如何写出Ｖｅｒｉｌｏｇ ＨＤＬ可综合风格的ＲＴＬ（Ｒｅｇｉｓｔｅｒ Ｔｒａｎｓｆｅｒ Ｌｅｖｅｌ）级语言描述的程序。     

虚拟仪器B型超声诊断仪的硬件系统设计

提出了虚拟仪器B型超声波诊断仪的概念和体系结构,介绍了虚拟仪器结构的B型超声波诊断仪的硬件系统设计与实现.着重介绍了仪器硬件的一些关键电路设计,以及提高仪器性能的途径和方法.     

基于Verilog HDL的DDS相位累加器的一种优化设计

通过对基于VerilogHDL的DDS相位累加器的传统设计方法的对比分析,提出了应用流水线技术加法器与寄存器结合在一起进行相位累加器设计的方案,该方案既具有提高速度又具有节约资源的优点,并且以一个八位相位累加器为例,给出了实验仿真结果。