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中国弯颈霉Tolypocladium sinense Li与雪白弯颈霉Tolypocladium nivcum(Rostrup)Bissct的孢子悬浮液经γ-射线Co~(60)辐射诱变后,产环孢菌素的得率及抑菌活性均明显提高.用黑曲霉Aspergillus niger和弯孢霉Curvularia lunata作为致敏菌。检测提取物中环孢菌素的抗菌活性,提取物抑制了致敏菌的菌丝生长和孢子形成.环孢菌素产生菌深层发酵培养基是蛋白胨—葡萄糖培养基,发酵周期12天.尔后发酵液用等体积的乙酸乙酯萃取,减压浓缩,每升发酵液获环孢菌素粗制品35—87mg. 相似文献
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Wu Jianyong 《中国工程科学》2014,12(1):2-9
Cordyceps sinensis (Cs) or Dongchongxiacao in Chinese is a rare and precious medicinal mushroom. In this work, a Tolypocladium sp. fungus named Cs-HK1 was isolated from a natural Cs fruiting body, and its ultraviolet (UV), infrared (IR) and high-performance liquid chromatography (HPLC) spectra all exhibited high degrees of matching to those of natural Cs, indicating the similarity of chemical composition. A crude polysaccharide (PS) fraction isolated from Cs-HKI fungal mycelium showed significant antitumor activity, inhibiting melanoma tumor growth in mice. The PS-treated mice showed significantly higher serum interferon-y (IFN-y) level and higher proliferation rate of lymphocytes ex vivo than the control animals, suggestive of the immunomodulatory activity of the PS fraction. In cell cultures, the PS fraction caused a significant and dose-dependent inhibitory effect on the proliferation of two cancer cell lines, B16/neu mouse melanoma and MDA-MB-231 human breast cancer cells, but not on normal spleen lymphocytes in vitro. Most of these bioactivities of the Cs- HK1 fungus were comparable to or even greater than those of natural Cs. The results showed that Cs-HK1 fungus may be a functional substitute for the natural Cs in health food and herbal medicine. 相似文献
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弯颈霉属一新种的研究 总被引:2,自引:0,他引:2
李兆兰 《南京大学学报(自然科学版)》1986,(3)
云南省迪庆藏族自治州产的冬虫夏草经组织分离获得一纯种,在几种琼脂培养基上形成瓶梗及瓶梗孢子。瓶梗基部呈球形或椭园形膨大,瓶颈细长而弯曲,经鉴定隶属于弯颈霉属Tolypocladium W.Gams,但与弯颈霉属已知种有明显区别,故认为是弯颈霉属一新种,命名为中国弯颈霉Tolypocladium sinensis Li sp. nov,弯颈霉属的种类在中国从未发现和报导过,本文首次报导。中国弯颈霉经深层培养,培养基为蛋白胨葡萄糖,无机盐类,生长素等,26℃,PH5.2,振速100次/分,发酵周期12天。其分生孢子梗,瓶梗及瓶梗孢子的形态发生很大变异。发酵液经乙酸乙酯提取,减压浓缩,获粗提物,对几种丝状真菌具抑制作用,并与环孢菌素标准样品进行了对照和比较,证实中国弯颈霉能产生环孢菌素。 相似文献
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