微孔钨钼磷酸盐的红外光谱与热稳定性

用红外光谱法、热重法及示差扫描量热法研究了微孔钨钼磷酸盐的红外光谱特征和热稳定性．测试结果表明，该化合物是一种热稳定性高的微孔材料．     

裂解急冷油的热稳定性研究

在急冷循环过程中，裂解急冷油的粘度逐渐升高，往往给乙烯装置正常运行带来严重影响．以齐鲁石化公司烯烃厂乙烯装置急冷系统为对象进行了急冷油的热稳定性研究．认为急冷油粘度升高的内在原因是急冷油中的芳香分在急冷过程中发生缩合反应，不断生成胶质及沥青质．鉴于急冷油中芳香分的基本来源是裂解原料中的芳烃，因此应尽可能采用芳烃含量少的裂解原料．     

复合型半导体CO气敏元件的研究

根据半导体气敏元件电路工作原理，设计了一种新型的气-气复合型的半导体CO敏感元件，推导出了其灵敏度、选择性及热稳定性的关系式，指出了构成高选择性及好的热稳定性的复合气敏元件需满足的条件，并在实验中得到验证。     

How do thermophilic proteins deal with heat?

Recent years have witnessed an explosion of sequence and structural information for proteins from hyperthermophilic and thermophilic organisms. Complete genome sequences are available for many hyperthermophilic archaeons. Here, we review some recent studies on protein thermostability along with work from our laboratory. A large number of sequence and structural factors are thought to contribute toward higher intrinsic thermal stability of proteins from these organisms. The most consistent are surface loop deletion, increased occurrence of hydrophobic residues with branched side chains and an increased proportion of charged residues at the expense of uncharged polar residues. The energetic contribution of electrostatic interactions such as salt bridges and their networks toward protein stability can be stabilizing or destabilizing. For hyperthermophilic proteins, the contribution is mostly stabilizing. Macroscopically, improvement in electrostatic interactions and strengthening of hydrophobic cores by branched apolar residues increase the enthalpy change between the folded and unfolded states of a thermophilic protein. At the same time, surface loop deletion contributes to decreased conformational entropy and decreased heat capacity change between the folded and unfolded states of the protein. Received 28 February 2001; received after revision 26 March 2001; accepted 27 March 2001     

降解结晶纤维素极耐热葡聚糖酶重组菌的构建

极耐热纤维素酶在纤维素生物转化中具有潜在的开发前景,但极耐热酶缺乏对天然纤维素的分解能力。采用基因工程方法,将极耐热内切葡聚糖酶Cel12B基因和同样极端嗜热菌来源的纤维素结合域(CBD)区域融合,构建重组质粒pET-20b-Cel12B-CBD,转化至大肠杆菌JM109(DE3)诱导表达后,融合基因表达产物对结晶纤维素具有一定的分解能力。