排序方式: 共有40条查询结果,搜索用时 15 毫秒
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小菜蛾Plutella xylostella羧酸酯酶、酰胺酶及乙酰胆碱酯酶的研究 总被引:2,自引:0,他引:2
研究结果表明,小菜蛾(Plutella xylostella)乙酰胆碱酶、酰胺酶的最适pH值分别为8.0和8.5。pH值对闳酸酯酶活力影响较小,酰胺酶和羧酸酯酶反应速度在反应开始60钟内,仍在线性范围,乙酰胆碱酯酶反应速度在反应开始的8分钟内呈线性关系。羧酸酯酶、酰胺酶和乙酰胆碱酯酶的Vmax分别为551.81(nmol/mg,mln)、5.25×10~(-4)(μmol/mg.min)和20.36(nmol/mg.min);km分别为5.52×10~(-5)(mol)、1.18×10~(-3)(mol)和8.33×10~(-3)(mol)。 相似文献
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在湿度定义为干、湿球温度差为3℃、光照强度10000—12000lx、光照周期14L:10D条件下,探索饲养温度(X:24—36℃)之变化对小菜蛾(Plutella xylostella)4龄幼虫乙酰胆碱酯酶(Ache)和羧酸酯酶(CarE)活力(Y)的影响效应。实验结果表明,活力指数随着饲养温度的升高而降低,两者之间存在显著的线性相关关系:温度与乙酰胆碱酯酶(AchE)活力两者间的相关关系函数式为Y=0.3327—0.0068X;与羧酸酯酶(CarE)活力的为Y=3.1278—0.0780X。 相似文献
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杨玉英 《宁夏大学学报(自然科学版)》2014,(4):366-369
研究草麻黄提取产物对小菜蛾生物活性的影响.结果表明,当提取液处理浓度为500g/L时,氯仿、甲醇、乙醇、丙酮和石油醚的提取物可引起小菜蛾幼虫死亡,其死亡率分别为82%,64%,62%,48%,46%,且都随提取物浓度的降低而减小;5种溶剂的提取物对小菜蛾的卵都具有一定的毒杀作用,作用最强的为高浓度(500g/L)的氯仿提取物;卵的孵化率随提取物浓度的升高而显著降低,提取物对小菜蛾的初孵幼虫具有一定的杀死作用,当浓度为500g/L时,氯仿提取物可引起90%的小菜蛾初孵幼虫的死亡. 相似文献
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采用浸渍叶碟法研究天葵块根提取物对菜青虫、小菜蛾生长发育的影响.结果表明,天葵块根提取物对菜青虫、小菜蛾具有明显的生长发育抑制作用,800mg/L对菜青虫、小菜蛾的生长发育抑制率分别为67.18%,68.31%.该提取物对菜青虫、小菜蛾生长发育抑制IC_(50)分别为389mg/L,251mg/L,对试虫的体重增加也有显著影响,处理组的平均体重增加值显著低于对照. 相似文献
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CHEN ZengLiang FANG YuLing ZHANG ZhongNing 《科学通报(英文版)》2007,52(10):1365-1371
Microcapsules of sex pheromone of the diamondback moth (DBM), Plutella xylostella (Lepidoptera: Plutellidae) were synthesized through complex coacervation using gelatin and gum arabic as wall-forming materials. The encapsulated pheromone released from these microcapsules into the air was monitored over six weeks in the field. Results of a field trial show that the attractiveness of microcapsules was superior to that of rubber septa loaded with the same amount of pheromone. The mating disruption efficiency of the pheromone treatment was estimated through comparing numbers of moths captured in pheromone-baited traps placed in pheromone treatment fields and pesticide treatment fields. The estimated mating disruption efficiency of the pheromone treatments ranged from 76.94% to 98.67% during the season. This study shows that pheromone microcapsules might provide a new method for P. xylostella control. 相似文献
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为考察茶皂素灭杀害虫效果和对枯萎病原真菌的抑菌能力,采用浸液杀虫法和土壤杀虫法测定不同浓度的茶皂素对于蚯蚓的急性毒性效应;采用叶片药膜法考察茶皂素对小菜蛾拒食效果与毒力效应,采用平板法考察茶皂素对于枯萎病原真菌的抑制作用.试验结果表明,浸液杀虫法灭杀蚯蚓的有效茶皂素浓度为0.3 mg/mL,土壤杀虫法7 d内的LD50为0.8 mg/mL;茶皂素对于小菜蛾主要是拒食作用;高浓度试药(>0.39 mg/mL)的茶皂素对枯萎病原真菌有一定的抑制作用. 相似文献
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Enzymatic processing of pheromones and pheromone analogs 总被引:3,自引:0,他引:3
G. D. Prestwich S. McG Graham M. Handley B. Latli L. Streinz M. L. J. Tasayco 《Cellular and molecular life sciences : CMLS》1989,45(3):263-270
Summary Pheromone perception requires rapid enzymatic degradation of the active chemical signal in the sensory hairs. Three insects are used to illustrate chemical approaches to studying the degradation of pheromones by antennal enzymes. First, hydrolysis of acetate and haloacetate esters is examined in the diamondback moth,Plutella xylostella. Second, aldehyde oxidation and the selective inhibition of the oxidase and dehydrogenase activities are described for the tobacco budworm moth,Heliothis virescens. Finally, a stereochemical analysis of the epoxide-hydrase catalyzed addition of a water molecule to the oxirane ring of disparlure is described for the gypsy moth,Lymantria dispar. 相似文献
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银杏外种皮中银杏酚酸对小菜蛾幼虫的拒食及毒杀作用 总被引:14,自引:0,他引:14
小菜蛾(Plutella zylostella L.)是一种全球性的重要害虫,其抗药性引起国际学术界的广泛关注,而开发高效、低毒、低残留、无污染的生物农药成为了国内外农药开发的主流.报道了从废弃的银杏(Ginkgo biloba L.)外种皮中分离到的银杏酚酸对小菜蛾的拒食和毒杀作用.实验采用皂化和酯化反应及柱层析方法从银杏外种皮中分离获得银杏酚酸,并对1~3龄小菜蛾幼虫进行生物测定.生测结果表明银杏酚酸对小菜蛾有较好的拒食和毒杀作用.当处理的质量浓度为6.25mg/L时3龄幼虫的非选择性拒食率在24,48h后分别达到77.8%,94.7%以上.处理时间达到48h时,1~2龄幼虫的LC50为1.31mg/L,3龄幼虫的LC50为6.07mg/L.这表明了银杏外种皮有很好的开发前景,将为今后新型植物农药的开发和银杏废弃物的综合利用提供依据. 相似文献
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