Estrogen and accessory sex gland lipids

Summary Administration of estradiol-17 elicited differential responses on accessory sex glands of rats. In caput epididymis, the estrogen treatment led to an accumulation of glycerides and phospholipids while in cauda epididymis, there was no significant change. However, in seminal vesicles, depletion of phospholipids was observed In prostate, the treatment, resulted in an accumulation of glycerides.Part of Ph.D. Thesis. Umapathy, E., Thesis. University of Madras, India, 1977.     

子宫内膜异位症的子宫内膜雌激素受体和孕激素受体mRNAs的表达

目的 :探讨雌激素受体 (ER)和孕激素受体 (PR)在子宫内膜异位症 (内异症 )子宫内膜的表达。方法 :利用大鼠内异症动物模型 ,采用逆转录聚合酶链反应 (RT -PCR)技术 ,检测子宫内膜ER和PRmRNAs的表达情况。结果 :内异症模型组大鼠异位内膜ER、PRmRNAs的表达低于在位内膜和对照组正常子宫内膜 ,与后两者比较差异有显著性意义 (P <0 0 1) ;而模型组在位内膜ER、PRmRNAs的表达与正常对照组比较差异无显著性意义 (P >0 0 5 )。内异症模型组异位内膜ER/PRmRNA大于在位内膜和正常子宫内膜ER/PRmRNA(P <0 0 1)。结论 :内异症大鼠异位内膜ERmRNA表达的相对增高在内异症的发生与发展中起着一定的作用     

逍遥散治疗原发性痛经的疗效评价及内分泌调节机制

原发性痛经（PD）是育龄期女性最常见的妇科疾病。以PD患者为研究对象,通过比较治疗前后女性激素水平和子宫形态学的改变,旨在明确逍遥散对PD患者内分泌水平的影响及其治疗机制。利用彩色多普勒技术测量治疗前后的子宫形态学参数,检测血清雌二醇（E2）、孕酮（P）等血清激素指标,疗效指标包括疼痛视觉模拟评分（VAS）和Cox痛经症状量表（CMSS）。结果显示,患者治疗后的血清E2水平、子宫前后径与内膜厚度、VAS、CMSS较治疗前显著降低,而血清P水平较治疗前显著提高,血清E2与子宫前后径之间呈正相关,血清P与内膜厚度之间呈负相关,血清P、E2与VAS、CMSS之间存在相关性;说明逍遥散对PD的疗效确切,其可通过调节E2、P水平降低PD患者的子宫前后径及内膜厚度、改善痛经症状及持续时间。     

小白鼠动情周期及妊娠早期（1—6天）子宫雌激素受体含量的变化

本文实验初步证明,妊娠D_1天的小鼠子宫ER。含量稍高(142.4±42.1 fmol mg蛋白)。D、降至117.7±45.4fmol/mg蛋白。D_3和D_4分别上升到158.9±40.3fmol/mg蛋白及162.1±34.8fmol/mg蛋白.D_5降为137.7±52.7fmol/mg蛋白(P<0.01)。D_6急增至182.4±44.9fmol/mg蛋白(P<0.01),达到妊娠D_1～D_6的峰值,在动情周期中,前期ER(?)含量为188.6±49.2fmol/mg蛋白,动情期则降至109.7±47.1fmol/mg蛋白(P<0.01)。后期较低(106.6±28.4fmol/mg蛋白),间期又回升到163.5±41.2fmol/mg蛋白,反映了ER_c的规律性变化是受内源性雌激素周期性地调控的,妊娠D_1的小鼠子宫湿重较高(126.7±56.8mg).此后D_2～D_4呈下降趋势。D_5极显著地上升至138.0±29.4mg。D_6又急下降(P<0.01)。在动情周期中,小鼠子宫湿重从前期的67.6±36.5mg上升至动情期的124.2±72.4mg。后期降为74.1±57.3mg。间期则降至最低水平。     

Initiation of cancer and other diseases by catechol ortho-quinones: a unifying mechanism

Exposure to estrogens is a risk factor for breast and other human cancers. Initiation of breast, prostate and other cancers has been hypothesized to result from reaction of specific estrogen metabolites, catechol estrogen-3,4-quinones, with DNA to form depurinating adducts at the N-7 of guanine and N-3 of adenine by 1,4-Michael addition. The catechol of the carcinogenic synthetic estrogen hexestrol, a hydrogenated derivative of diethylstilbestrol, is metabolized to its quinone, which reacts with DNA to form depurinating adducts at the N-7 of guanine and N-3 of adenine. The catecholamine dopamine and the metabolite catechol (1,2-dihydroxybenzene) of the leukemogen benzene can also be oxidized to their quinones, which react with DNA to form predominantly analogous depurinating adducts. Apurinic sites formed by depurinating adducts are converted into tumor-initiating mutations by error-prone repair. These mutations could initiate cancer by estrogens and benzene, and Parkinson's disease by the neurotransmitter dopamine. These data suggest a unifying molecular mechanism of initiation for many cancers and neurodegenerative diseases and lay the groundwork for designing strategies to assess risk and prevent these diseases. Received 4 September 2001; received after revision 28 November 2001; accepted 2 December 2001     

Captivity affects behavioral physiology: Plasticity in signaling sexual identity

Summary Little is known about the link between captivity, physiology, and behavior in wild-caught vertebrates. Anecdotal evidence suggests that hormonal changes are responsible for behavioral changes in wild animals brought into captivity. Studying the effects of captivity on reproduction is hampered because wild animals often fail to exhibit sexual behavior under captive conditions. In weakly discharging electric fish, field studies have reported sex differences in electric organ discharges which are rarely seen in the laboratory. I now report the results of a series of laboratory investigations which show thatGnathonemus petersii exhibits seasonal, hormone-dependent, phasespecific sex differences in electric organ discharges. Captivity dramatically alters and may even reverse these sex differences as a result of rapid changes in endogenous plasma hormone levels. These findings have broad implications for research on animal physiology and behavior performed in laboratory settings.     

云南梁河傣族育龄妇女雌激素α受体基因多态性分析

检测分析云南特有少数民族傣族育龄妇女雌激素受体基因多态性,为进一步研究不同地域种族女性ERα基因多态性与育龄期疾病及退性行疾病关系提供依据.以164例云南梁河傣族育龄妇女为研究对象,PCR-RFLP方法检测分析其ERα基因rs2234693(PvuⅡ)、rs9340799(XbaⅠ)位点的基因多态性,结果显示rs2234693位点基因型有CC、CT、TT 3种,其分布频率依次为17.1%、42.7%、40.2%;rs9340799位点基因型有AA、AG、GG 3种,其分布频率依次为66.5%、24.4%、9.1%,基因型分布频率与其他种族有所不同.云南梁河傣族育龄妇女rs2234693、rs9340799位点多态性存在种族差异.     

Ontogeny of the sex steroid and prolactin receptors in the male rat adrenal gland

Summary Cytosolic estrogen and androgen receptors and membrane prolactin-binding sites in the male adrenal glands showed a definite pattern during sexual development. The level of sexual steroid receptors paralleled adrenal growth, whereas prolactin binding reached its maximum value in mature rats.Lüthy, I. A., Predoctoral Fellow from the Consejo Nacional de Investigaciones Cientificas y Técnicas and Calandra, R. S., Research Career Awardee from the Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina.Acknowledgments. We would like to thank Mrs D. Bas and Mrs D. B. Destéfano for the skillful technical assistance and the secretarial work, respectively. This work was partially supported by the Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina (CONICET), and the Comisión Nacional de Energía Atómica.     

雌激素受体ERβ配体结合区的异源表达与纯化

为研究环境化合物与雌激素受体的相互作用,在大肠杆菌中异源表达了人雌激素受体ERβ的配体结合区(hERβ-LBD)蛋白。在诱导剂(IPTG)浓度为0.2mmol.L-1,培养温度25℃,诱导时间为8h的优化表达条件下,经亲和色谱法纯化后,hERβ(LBD蛋白的产量可达236mg.L-1.     

环境污染物的抗雌激素效应及机制研究进展

 列举了10 余种具有抗雌激素效应的环境污染物, 从拮抗E₂水平、拮抗E₂功能、拮抗雌性性征3 个方面阐释了这些物质的抗雌激素效应, 并进一步论述了其在E₂合成、运输、受体结合及代谢等方面发挥抗雌激素效应的机制。展望了环境污染物的抗雌激素效应及相关机制的研究方向。