排序方式: 共有5条查询结果,搜索用时 15 毫秒
1
1.
2.
养殖中华鳖一种球形病毒的电镜观察 总被引:5,自引:0,他引:5
应用电镜超薄切片技术,观察福建省四个主要养鳖市县现场采集的鳖出血病样品.首次发现了一种感染养殖中华鳖的球形病毒(Trionyxsinensisspherovirus,TSSV).该病毒分布在病鳖的肺、胃、咽喉粘膜和腹甲皮层.主要靶细胞是这些组织的小血管和毛细血管的内皮细胞.TSSV粒子近球形,直径35~39nm,无囊膜包被,有的成群聚集在内皮细胞质中,有的由单位膜包裹在包涵体中.病毒感染的内皮细胞病变明显.还讨论了TSSV与出血病的关系. 相似文献
3.
《Journal of Natural History》2012,46(11):1713-1735
Using morphometric studies of colonial (clonal) organisms such as Bryozoa grown as replicates in controlled laboratory experiments, phenotypic variation (hard part morphology) can be partitioned into its genotypic and environmental (ecophenotypic) components. The interaction between these, i.e. different genotypic responses to the same environmental change, can also be recognized. Palaeobiological studies are inherently constrained by species concepts based on morphotypes (preserved morphological phenotype). Uncertainties associated with fossil species concepts restrict the deductive resolution potential of fossil taxa in discussions of the broader biological questions of species evolution, ecology, biogeography and phylogeny. The relationship between species-level morphological variation and genetic variation in modern taxa is central to evaluating the viability of fossil morphotypes as biological species. Results from a preliminary study of three genotypes of Electra pilosa L., grown as replicate colonies in tanks comprising different microenvironmental conditions, allow for direct evaluation of morphospecies concepts. Numerical analyses (Cluster Analysis, Principal Component Analysis and Two-way ANOVA) of six morphometric characters demonstrated a strong Genotype control over zooid morphology and limited environmental (Tank) effects with minimal environmental differences among tanks. No significant Genotype by Tank (=environment) interactions were found for any characters. These results demonstrate that it is possible to quantify the extent to which a given trait is plastically expressed in different environments. The strong degree of correlation between morphology and genetics for this species is encouraging for the use of morphotypes as proxies for biological species. 相似文献
4.
Mg ions can enhance the proliferation and redifferentiation of chondrocytes and the osteogenic differentiation of osteoblasts at specific concentrations, respectively. However, degradation of Mg alloys at varying degradation rates could lead to complex changes in the surrounding tissue environment, such as changes in the dynamic concentration of Mg ions and subsequent p H value. Considering the above mentioned factors, the comprehensive effects of Mg alloys on chondrocytes and osteoblasts behaviors have not yet been optimized. In this study, we evaluated the effects of Mg–1Ca microspheres on cell behavior with an aim to optimize conditions favorable for both cell types. Cells were cultured with Mg–1Ca microspheres prepared using the following concentrations: 250 μg/ml, 500 μg/ml and 1000 μg/ml. At specific time points,cytotoxicity, expression of specific genes and extracellular matrix deposition by cells(Alizarin Red Staining of osteoblasts and Alcian blue staining for chondrocytes) were evaluated. The experimental results revealed that Mg–1Ca microspheres prepared at a concentration of 250 μg/ml were optimum for both cell types, where chondrocytes were found to be in hypertrophy state while osteoblasts in close proximity to the microspheres showed osteogenetic differentiation. Interestingly, a slight change in osteoblasts behavior was observed nearer to and at a relative distance away from Mg–1Ca microspheres, an important observation for administering the application of microspheres as potential scaffolds. 相似文献
5.
采用定氮法、电泳法及氨基酸自动分析仪检测红驼和白驼血清蛋白质的各组分含量、分子量及水解氨基酸的种类.结果表明红驼和白驼血清蛋白组成基本一致.薄膜电泳分离得到的5条蛋白区带没有显著差异,SDS-PAGE分离均可得到分子量在0.80×104~11.2×104范围内的8条蛋白区带,这些蛋白共由17种氨基酸按一定的百分比构成.二者的差异主要表现在不同氨基酸种类的含量和高分子量蛋白区带的分布状态.揭示了双峰驼在定向培育过程中血清蛋白表型发生的变异程度 相似文献
1