三七叶甙对兔血小板聚集功能的影响

 应用Born氏比浊法分别测定三七叶甙(Panax notoginseng folium saponins)对花生四烯酸(arachidonic acid,AA)、血小板活化因子(platelet activating factor,PAF)和腺苷二磷酸(adenosine diphosphate,ADP)诱导的兔血小板聚集功能的影响.实验结果表明:三七叶甙在体外显著抑制ADP诱导的血小板聚集,其半数抑制浓度(medium inhibitory concentration,IC₅₀)为89.4mg/L;三七叶甙静脉注射也能明显降低ADP诱导的兔血小板聚集率,且呈剂量-效应关系.提示三七叶甙体内外均明显抑制ADP诱导的兔血小板聚集,而对AA和PAF诱导的兔血小板聚集无明显影响.     

Drug susceptibility of PCA in WBB6F1-W/Wv mice

Our previous study revealed that passive cutaneous anaphylaxis (PCA) can be produced in congenitally mast cell-deficient WBB6F1-W/W^v (abbreviated as W/W^v) mice on sensitization with undiluted or slightly diluted allogeneic and xenogeneic antisera but not on sensitization with allogeneic monoclonal immunoglobulin (Ig)E and IgG1 antibodies regardless of the antibody concentration [1]. In view of these findings, the present study was conducted to characterize PCA in this strain from its drug susceptibilities using mast cell-bearing WBB6F1-+/+ (abbreviated as +/+) and B6D2F1 mice as references. PCA in W/W^v mice mediated by a low dilution (1  4) of hyperimmune serum to bovine serum albumin of the B6D2F1 mouse origin was markedly suppressed by CV-6209, an antagonist of platelet-activating factor (PAF), but not by antihistamines such as cyproheptadine and oxatomide. In contrast, PCA in +/+ and B6D2F1 mice mediated by a high dilution (1  128) of the anti-serum (virtually by IgG1 antibody) was nearly completely suppressed by antihistamines but not by CV-6209. A remarkable difference between PCA in W/W^v and reference mice was also observed in the susceptibility to monoclonal anti mouse granulocyte (Gr-1) antibody PCA in W/W^v mice was potently suppressed by the 1- to 3-day pretreatment with this antibody but that in references was not at all. Putting these present results together with the previous finding that anti-granulocyte antibody greatly reduces circulatory Gr-1⁺ leukocytes, 1 to 3 days after the treatment [2], it is highly probable that PCA in W/W^v mice mediated by some antibody isotypes other than IgE and IgG1 is produced by PAF mainly released from Gr-1⁺ cells, while IgG1 antibody-mediated PCA in mast cell-bearing reference mice is evoked by histamine derived from mast cells. PCA homologous to that in W/W^v mice could also be produced in the reference mice on sensitization with undiluted or slightly diluted antiserum, when generalized blueing due to excess IgG1 antibody was removed by the oxatomide treatment be fore the antigen challenge. Received 10 December 1997; received after revision 2 February 1998; accepted 23 February 1998     

PAF处理含普拉黄染料废水的研究

研究了马铃薯渣制备的活性纤维(PAF)在染料废水中对普拉黄的静态吸附和动态吸附及PAF的用量、吸附温度、时间等因素对其吸附能力的影响,并应用于实际染料废水处理.实验结果表明,活性纤维PAF对含普拉黄染料的废水的处理效果较好.     

类风湿关节炎活动期患者血清血小板活化因子含量测定及临床意义

目的探讨血小板活化因子(PAF)在类风湿关节炎(RA)活动期中的水平变化及临床意义。方法采用酶联免疫吸附方法检测43例活动期RA患者血清PAF含量,并与30例健康人血清PAF含量进行比较。结果RA活动期组血清PAF含量(116.02±20.15)明显高于健康对照组(44.27±18.98),差异具有非常显著性意义(t=15.325 6,P<0.001)。结论活动期RA患者血清PAF水平明显升高,可作为反映RA患者风湿活动的评估指标之一。     

基于CNN的心冲击信号阵发性房颤自动检测方法

阵发性房颤具有发作突然且时间短的特点，而目前其临床诊断方法——心电信号，不适于日常监护，因此，提出一种基于心冲击信号(ballistocardiogram，BCG)的非接触式房颤自动检测方法.研究不同输入数据长度与不同网络深度的匹配关系，获取应用一维卷积神经网络(convolutional neural network，CNN)检测阵发性房颤的最优组合.通过2000组数据的测试，所提模型的最佳性能为:测试准确性94.8%、敏感性97.2%、特异性92.7%，为基于BCG信号的心律失常检测与远程日常家庭监护提供了可能性.     

基于压缩感知的宽孔径SAR成像

宽孔径SAR数据包含目标不同姿态角散射信息,实现宽孔径SAR成像对目标轮廓重构与解译识别具有重要价值.本文首先提出点模糊函数(point ambiguous function,PAF)研究影响压缩感知SAR成像的因素,然后用模型失配函数(model mismatch fucntion,MMF)分析宽孔径测量条件下点散射模型失配对成像性能的影响.最后根据上述结论,选择发射信号参数改善测量矩阵性能,利用广义似然比检验方法克服模型失配对成像性能的影响.实验结果验证了本文结论的正确性和成像算法的有效性.     

马铃薯渣制备纤维PAF对含阳离子红染料废水的处理研究

研究了马铃薯渣制备的活性纤维(PAF)在染料废水中对阳离子红的静态吸附和动态吸附以及PAF的用量、吸附温度、时间等因素对其吸附能力的影响.结果表明,在实验条件下,温度、浓度、吸附时间及PAF的用量是影响吸附脱色的主要因素;PAF对阳离子红染料分子的静态吸附量Q为11.10 mg/g;动态吸附的吸附量比同时间下的静态吸附量要大,采用PAF装柱法有望进行该废水的处理.     

抗PAF单链抗体的基因构建及蛋白质3D模建

构建抗PAF单链抗体的基因并通过计算机模建分析几种不同连接类型的单链抗体空间结构差异。采用重叠延伸PCR方法扩增了VH和VL基因,形成抗PAF的VH-Linker-VL(ScFv)基因产物,并经菌落PCR和测序分析鉴定,通过Internet对其3D结构进行了模建与分析,研究结果表明：PCR产物电泳可见一条与目的基因大小一致的片段,经菌落PCR和测序分析鉴定,抗PAF单链抗体的基因构建成功;VH和VL在单链抗体中的顺序对单链抗体3D的空间结构有影响。     

银杏有效化学成分的药理及其应用

银杏为银杏科落叶乔木,其叶、果实、种子均有较高药用价值,且为我国特有孑遗树种。阐述银杏的生物学特性、化学成分、药理、临床应用和栽培管理,为更好的利用和栽培银杏提供理论依据和方法。