排序方式: 共有7条查询结果,搜索用时 375 毫秒
1
1.
柚子中柚皮甙提取工艺研究 总被引:4,自引:0,他引:4
研究了柚子中柚皮甙提取工艺.以柚皮甙的含量为指标,采用L9(33)正交试验设计优选柚子中柚皮甙最佳提取工艺条件.优选得到的最佳提取工艺:提取温度为90℃,乙醇浓度为70%,提取时间为90min ,液固比为25:1.在最优提取条件下提取3次,柚皮甙的提取率可达83.32%. 相似文献
2.
3.
骨碎补有效成分柚皮甙对人骨髓间充质干细胞的影响 总被引:6,自引:0,他引:6
目的探讨骨碎补的有效成分枘皮甙对人骨髓间充质于细胞(hMSCs)增殖、分化的影响。方法将骨碎补的有效成分柚皮甙以及成骨诱导液(地塞米松、维生素C、β-甘油磷酸钠)与hMSCs共同体外培养,用倒置显微镜观察细胞生长情况、CCK-8法检测细胞的毒性和增殖作用、碱性磷酸酶(ALP)活性测定、von kossa钙结节染色。结果50μg/L柚皮甙对细胞无毒性、能促进hMSCs增殖,碱性磷酸酶(ALP)活性、von kossa钙结节染色与卒白对照组比较有明硅差异(P〈0.05)。结论柚皮甙对hMSCs有保护作用并能促进其增殖、分化。 相似文献
4.
《自然科学进展(英文版)》2022,32(4):498-503
Naringin is a predominant flavanone in grapefruit and shows a variety of biological effects such as antioxidative, anticancer, anti-inflammatory and antibacterial activity. However, its application in pharmaceutical field is limited by low water solubility, poor bioavailability and instability. To overcome the problem, naringin has been encapsulated in poly(lactic-co-glycolic acid) (PLGA) polymer by emulsion-diffusion-evaporation method in this work. Moreover, naringin loaded PLGA nanospheres were characterized by scanning electron microscope (SEM), dynamic light scatter method (DLS), fourier transform infrared (FTIR) spectra, UV–vis spectra and fluorescence spectra of DNA-EB competition displacement. The mean diameter of PLGA nanospheres and NRG/PLGA nanospheres was 123 ?± ?25 ?nm and 137 ?± ?30 ?nm, respectively. The drug encapsulation efficiency was 86.4% while the drug loading rate was 22.3%. The fluorescence spectra of the competitive DNA-binding experiments revealed that the functional activity of naringin was retained after loaded in PLGA. It is revealed that the initial burst effect happened in the initial 24 ?h and followed by sustained release lasting for 10 days. Moreover, the nanospheres exhibited strong antibacterial activity, and 99.9% of E. coli and S. aureus were killed when treated with naringin loaded PLGA nanospheres at the concentration of 0.2 ?mg ?mL?1 within 24 ?h. Furthermore, the viable cells remained only 48% when the concentration of NRG/PLGA nanospheres was 32 ?μg ?mL?1 and NRG/PLGA nanospheres was important for inhibition of cancer cells. It is concluded that the stable naringin loaded PLGA nanospheres could have potential application in food industry and nanomedicine field. 相似文献
5.
从丰富易得的柚皮苷(Naringin)1出发,通过酸水解得到了柚皮素2.以柚皮素为先导化合物,通过4-位羰基与水合肼作用合成了中间体柚皮素腙3,进而与各种不同类型的醛缩合,合成了14个新型N-苯亚甲基柚皮素腙及类似物(4a~4n).MTT蛋白染色法体外抑制肿瘤增值活性测试显示化合物4c、4d、4h、4j、4n对胃癌细胞SGC-7901有一定的抑制作用. 相似文献
6.
7.
高效液相色谱法测定胃苏颗粒中橙皮苷和柚皮苷含量 总被引:1,自引:0,他引:1
目的:用高效液相色谱法测定胃苏颗粒中橙皮苷和柚皮苷的含量。方法:采用DiamonsilTMC18(4.6 mm×150mm,5μm)色谱柱;乙腈-磷酸水(pH=3.5)(22:78,V/)为流动相,流速为1.2 mL/min;.检测波长为283 nm。结果:待测组分与其他组分分离度良好(R>1.5),橙皮苷和柚皮苷线性范围分别为0.25~1.49μg和0.093~0.56μg,橙皮苷和柚皮苷的平均回收率分别为99.2%,98.8%,RSD分别为1.6%,2.3%。结论:高效液相色谱法是测定胃苏颗粒中橙皮苷和柚皮苷成分的一种简便、有效的方法。 相似文献
1