噬菌体展示禽流感病毒抗原变异性基因片段文库的构建

构建T7噬菌体展示禽流感病毒抗原变异性基因片段文库. 首先， 从Gene Bank中查找筛选禽流感病毒抗原变异性基因, 将其截短、 修饰、 简并后得到禽流感病毒抗原变异性基因微阵列. 其次， 将合成的禽流感病毒抗原变异性基因片段文库扩增、 酶切, 链接到双酶切后的T7噬菌体载体基因上, 构成重组噬菌体DNA. 最后， 重组噬菌体DNA经体外包装和扩增, 得到T7噬菌体展示文库, 并进行T7噬菌体展示文库滴度、 重组率和免疫活性测定. 实验结果表明, 从Gene Bank中查找、 筛选、 剪切和修饰共获得96 258条序列构建T7噬菌体展示文库, 原始文库滴度为3.6×107个菌落/mL, 重组率大于90%. 用禽流感病毒H5N1抗体进行捕获, 经聚合酶链式反应(PCR)鉴定, 得到理想目的条带, 证明噬菌体表面展示蛋白具有抗原活性, 可用于禽流感病毒感染患者的快速检测及抗原表位筛选.     

半刚性基层施工弯沉检测标准研究

根据重庆地区大量实测资料 ,对影响半刚性基层弯沉变异性的各主要因素进行了分析 ,并根据实际需要 ,对施工期实测弯沉的龄期与养生温度的影响进行了修正 ,得出可用于施工检测的弯沉控制标准     

A local positive feedback of the tropical Pacific ocean-atmosphere system on interdecadal timescales

SINCE THE 1990S, THE CLIMATIC VARIABILITY ON INTERDE- CADAL TIME SCALES BECAME THE FOCUS OF THE INTERNATIONAL CLIMATOLOGY RESEARCH MISSIONS[1―3]. ON TIME SCALES OF A DECADE OR MORE, THE OCEAN CIRCULATION PREDOMINATEDHEAT BALANCE AND HYDROLOGICAL CYCLE, S…     

Variability of surface circulation in the South China Sea from satellite altimeter data

11-year satellite altimeter sea surface height （SSH） anomaly data from January 1993 to December 2003 are used to present the dominant spatial patterns and temporal variations of the South China Sea （SCS） surface circulation through Empirical Orthogonal Function （EOF） analysis. The first three EOF modes show the obvious seasonal variations of SSH in the SCS. EOF mode one is generally characterized by a basin-wide circulation. Mode two describes the double-cell basin scale circulation structure. The two cells were located off west of the Luzon Island and southeast of Vietnam, respectively. EOF mode three presents the mesoscale eddy structure in the western SCS, which develops into a strong cyclonic eddy rapidly from July to September. EOF mode one and mode three are also embedded with interannual signals, indicating that the SCS surface circulation variation is influenced by El Nino events prominently. The strong El Nino of 1997/98 obviously changed the SCS circulation structure. This study also shows that there existed a series of mesoscale eddies in the western SCS, and their temporal variation indicates intra-seasonal and interannual signals.     

土壤有机质空间变异特征研究

以面积4．7hm^2的农田为研究区，采用网格法选取了92个观测点取样，测定0～15cm耕层土壤的有机质含量。结果表明，土壤有机质表现为强烈的空间自相关，有机质的空间变异性主要由结构性因素引起，其变异函数符合指数模型。     

汾河流域降水量及蒸发量的空间变异性分析研究

借助于地质统计学方法对汾河流域的多年平均降雨量和蒸发量的空间变异性进行了分析,揭示了多年平均降水量和蒸发量的空间变异特征,并应用Kriging方法进行空间插值.分析结果表明,汾河流域多年平均降水量和蒸发量在不同的分割距离范围内有不同的空间相关性,多年平均降水量具有比较明显的地带性,蒸发量分布变化地带性不强.     

土壤特性空间变异对降雨下渗影响的统计理论研究

本文利用饱和土壤导水率K_5的对数正态特性,通过函数转换方法建立了降雨下渗中积水时间f_p的分布和两个统计特征值(即平均下渗率及估计方差)的表达式。研究了土壤特性空间变异对降雨下渗的影响。结果表明,土壤特性空间变异程度越大,影响越大,在产流计算中如果不考虑这种影响,将会引起较大误差。     

Research progress in nonlinear analysis of heart electric activities

1INTRODUCTION THE WORLD IS NONLINEAR,AND THE HUMAN LIFE ACTIVITY IS ALSO NONLINEAR.FROM THE VIEWPOINT OF SYSTEM,HUMAN BEINGS(OR THE OTHER HIGHER ANIMAL)ARE THE MOST COM-PLEX SYSTEM IN THE NATURE.FOR SUCH A PHYSIOLOGICAL SYS-TEM WITH THE UTTERMOST COMPLEXI…     

Cir X-1的时变性质

利用RXTE在不同时期的观测数据，分析了Cir X-1功率密度谱和交叉谱。功率密度谱表明，如果Cir X-1是Z源，那么所采用的数据将处于Z源的不同态，即垂直水平分支态、水平分支态、正常分支态和耀斑分态。各个态上的交叉谱显示，随着吸积率的变化，Cir X-1的交叉谱由硬向软转化，这和Cir X-1在一个完整Z形状上的时变性质一致。