实验Beagle犬贾第鞭毛虫感染情况调查

为了掌握Beagle犬贾第鞭毛虫的感染情况，本实验室在2009年3月～2010年2月共抽样检测了910只实验Beagle犬，其中109只检测到了贾第鞭毛虫包囊，贾第鞭毛虫感染的总阳性率为11．98％。结果还表明，不同月份间差异极显著，不同性别间差异不显著，幼犬感染率大于成犬，不同年龄组间差异极显著。通过检测可掌握犬群中贾第鞭毛虫的感染情况，对及时诊断、发现并控制疾病的传播有积极的指导意义。     

以30日龄长爪沙鼠为介体建立犬贾第虫纯培养

将取自然感染犬贾第虫的德国牧羊犬的包囊利用蔗糖梯度离心-G1耐酸漏斗滤过法进行分离和纯化，经口感染给30日龄的沙鼠．8d后无菌取小肠上段，分离出滋养体，转入改良TYI—S-33培养基中37℃培养．72h后在管壁上形成了细胞单层，将培养物置肉汤及血琼脂平板培养，未见细菌及霉菌污染，为纯培养．     

试论"两虫"的可替代检测

从生物学和物理学两方面对新颁布的《城市供水水质标准》中非常重要的微生物指标隐孢子虫和贾第鞭毛虫的可替代检测指标进行了论述和比较,指出虽然还不能找到明确的定论,但研究表明,颗粒计数法仍是目前比较合适的可替代的“两虫”检测方法。     

Inactivation of Giardia by Ozone and Its Disinfect Mechanisms

This study was to investigate the effect of ozone(O3)inactivation on Giardia in water by the fluorescence staining method.In order to elucidate the dominant mechanisms of inactivation,cell surface or inner cell components damage were comparatively examined by scanning electron microscopy(SEM).Results suggested that O3had a stronger effect on inactivating capability.Firstly,when the concentration of O3was above 2.0 mg/L and the contact time was up to 5 min,it showed a significant inactivating effect.Secondly,the effect of turbidity on inactivation was also found to be significant in synthetic water;when turbidity increased,the inactivating effect decreased.Thirdly,the inactivating rates were improved with a temperature increase from 5 to 25℃,but decreased when the temperature were out of this range.The inactivating capability of O3was stronger under acidic conditions than alkalic conditions.Lastly,when the concentration of organic matter in the reactive system was increased,probably due to the competition between Giardia and organics on O3,the inactivating rate was decreased;in addition,the cellular morphology of Giardia varied with different contact times.At contact time of 30 s,cells were rotundity and sphericity;at 60 s they became folded,underwent emboly,and burst;and at 240 s,the cell membrane of Giardia shrinked and collapsed completely.     

Identification and evolutionary implication of four novel box H/ACA snoRNAs from Giardia lamblia

During the biogenesis of eukaryotic ribosomes, the nascent pre-rRNA undergoes a complex pattern of nu- cleotide modifications of its mature rRNA sequences, including 2′-O-ribose methylation and pseudouridyla- tion, which are critical for the biogenesis a…     

用酶联免疫吸附试验诊断蓝氏贾第鞭毛虫病免疫价值的研究

用蓝氏贾第鞭毛虫纯培养虫株和超声粉碎可溶性虫体部分作为抗原,采用ELISA方法检测人体感染蓝氏贾第鞭毛虫血清免疫抗体并讨论其寄生虫学诊断价值．检测65份成人贾第虫病血清,其中55份IgG和／或IgA阳性,阳性率84．62％．20份10岁以下儿童贾第虫病人血清无1份阳性．结果提示蓝氏贾第鞭毛虫感染产生的体液免疫反应存在有年龄依赖性,酶联免疫吸附试验用于蓝氏贾第鞭毛虫病免疫诊断具有局限性．