基因工程菌株EscherichiacoliC600（pRLK14）的流加培养

在１Ｌ搅拌发酵罐中对含有重组质粒ｐＲＬＫ１４的大肠杆菌进行了培养。结果表明，采用二段培养法，于３４℃增殖细菌，在对数增殖后期升温到４２℃进行诱导，可以高效表达基因产品半乳糖激酶。诱导期，醋酸浓度增长较快，模拟实验表明，控制醋酸浓度可进一步提高半乳糖缴酶产率。     

废热锅炉盘管的非正常损伤

By using optical microstructure(OM), X-ray diffractometer analyses and mechanical properties testing, the abnormal damage of pipe coil in remaining heat boiler has been investigated. The damage is the result of the melting of tube base metal at high temperature caused by the ignition inside the tube.     

高效凝胶渗透色谱测定硅油分子量及其分布

采用ＨＰＧＰＣ与蒸汽压渗透（ＶＰＯ）结合的方法研究了甲基苯基硅油等分子量及其分布测定方法。     

电动机受潮的几种处理方法

介绍了实际生产中电机干燥的常用方法及其适用范围,并对现场操作时的注意事项做了简要说明。     

消弧方法及其新思路

对当前社会普遍流行的两种消弧方法进行分析,提出用可调电抗器来灭弧的新思路。     

Presence of hsp65 in bacterial extracts (OM-89): A possible mediator of orally-induced tolerance?

Heat shock proteins (HSP) have been implicated in rodent models of autoimmunity, particularly arthritis, and there is suggestive though inconclusive evidence that they may also play a role in human autoimmune disease. The simplest hypothesis is based on molecular mimicry due to the amino-acid sequence homology between mammalian and microbial HSP. Recently OM-89, an extract of several strains ofEscherichia coli, has shown some efficacy in the treatment of rheumatoid arthritis (RA) when taken orally. Using species-specific antibodies, we show here that OM-89 contains the 65 kDa HSP (hsp65), while hsp65 was not detected in another bacterial extract containing other microorganisms, includingStaphylococcus aureus (OM-85). We suggest that if the human homologue of hsp65 is a relevant target antigen in the human disease, the efficacy of the preparation could be due to induction of oral tolerance or to switching the Th1 response towards Th2. Alternatively, even if the human hsp65 is not a target molecule in RA joints, OM-89 may evoke bystander suppression of joint inflammation via induction of TGF-secreting effector cells. These hypotheses should be tested in further studies.     

大肠杆菌编码区5′端碱基的统计分析

统计了大肠杆菌１２８８个编码序列开始的３３个碱基位点（１１个密码子）上各碱基出现的概率,发现第２、第３密码子各位点上的碱基概率分布与其它密码子上的碱基分布概率不一样;碱基Ｇ和Ｔ出现的概率从第４个密码子后呈现明显的３周期分布．我们还研究了这些位点上的碱基概率分布与基因表达水平的关系,发现高表达基因和低表达基因在第２、第３密码子各位点上,其各种碱基的概率分布是有区别的;高表达基因碱基Ｇ和Ｔ的概率分布３周期性更明显．     

一种新型可连续调节的消弧线圈

采用可连续调节的消弧线圈不仅解决了高压电感调节系统的价格昂贵和调节系统十分复杂的问题，还可以使经消弧线圈接地系统的优越性充分发挥。文中着重介绍了如何采用低耐压的晶闸管连续调节6-66kV的消弧线圈的一种新方法。对新型消弧线圈的电感调节量的计算进行了理论推导，并对其产生的稳态电流谐波分量进行了分析，用Saber软件对所设计的消弧线圈的理论依据进行了仿真实验验证和实物实验验证。     

纳豆激酶基因克隆及其在大肠杆菌中活性表达研究

以纳豆芽孢杆菌基因组DNA为模板，PCR扩增了纳豆激酶基因（natto kinase gene）中编码前肽、成熟肽的核苷酸序列（pro-NK），构建大肠杆菌表达质粒pTYB102，转化大肠杆菌ER2566。在IPTG诱导下，分别在15℃（14h）、30℃（3h）、37℃（2h）条件下培养，pTYB102均能表达出有活性的纳豆激酶。实验证实纳豆激酶基因得到活性表达需要Pro序列。SDS－PAGE表明，15℃和30℃和37℃培养表达的杂蛋白更少。薄层扫描测定表达的纳豆激酶占菌体总蛋白30％以上。     

各向异性磁介质中载流矩形线圈空间磁场

在给出的各向异性的毕奥－萨伐尔定律的基础上，求出载流矩形线圈在各向异性磁介质中的空间磁场，为研究磁各向异性的磁场提供了实例。