艾美耳属球虫卵囊基因组DNA不同提取方法的比较

为了获得高质量的艾美耳属（Eimeria）球虫卵囊基因组DNA,采用了4种不同方法提取3种艾美耳属球虫卵囊的基因组DNA．对比结果表明,采用机械法破裂球虫卵囊壁,用裂解液裂解孢子囊和子孢子,释放出DNA,在保持碱性的环境下（pH=8．0）,以苯酚-氯仿异戊醇抽提,乙醇沉淀,可获得高质量的DNA分子,经PCR检验为艾美耳属球虫卵囊基因组DNA．     

柔嫩艾美耳球虫感染对雏鸡肾脏病理损伤的探讨

14日龄海兰褐雏鸡80只,分为对照组和感染组。感染组每只鸡口服接种8.0×104个柔嫩艾美耳球虫孢子化卵囊,分别于接种后第0,3,6,9,12天,每组随机取鸡5只,心脏采血,测定血清尿酸和肌酐含量,同时取感染后第6天鸡的肾脏,观察其病理组织学变化。结果表明:感染组鸡的血清尿酸含量于感染后第3,6,9天显著升高了267.41%(p<0.01),81.41%(p<0.01)和52.09%(p<0.05),肌酐含量在感染后第3,6天也显著增加了63.61%(p<0.05)和81.80%(p<0.05);肾脏的实质细胞呈轻度的颗粒变性或水泡变性。提示:球虫感染导致雏鸡肾脏病理损伤。     

Suppression of phytohemagglutinin induced splenocyte proliferation during concurrent infection withEimeria nieschulzi andNippostrongylus brasiliensis

Summary Results suggest that infection withEimeria nieschulzi (Protozoa) interferes with splenocyte proliferation induced by infection withNippostrongylus brasiliensis (Nematoda).This study was supported by NIH MBRS Grant RR08012-15.     

寄主及季节变化对广东艾美虫感染的影响

通过对寄生乌鳢肠道及幽门盲囊的广东艾美虫的研究,揭示了寄主以及季节变化对此虫感染情况的影响作用,指出广东艾美虫感染率的最高值出现在寄主体长20～24.9cm中,且雄性寄主较雌性寄主更高。另从气候的季节变化规律分析,感染率春季最高,而感染强度秋冬季最高。     

阶段用药抗柔嫩艾美耳球虫的效力

将 70只 10日龄公雏随机分成 7组 ,每组 10只。Ⅰ组为不感染不给药组 ,Ⅱ组为感染不给药组 ,Ⅲ组为中药 (5 g/kg饲料 )组 ,Ⅳ组为地克珠利 (1mg/kg饲料 )组 ,Ⅴ组为马杜拉霉素 (5mg/kg饲料 )组 ,Ⅵ组为阶段性运用地克珠利和中药组 ,Ⅶ组为阶段性运用马杜拉霉素和中药组。除Ⅰ组的鸡只外 ,其余全部试验鸡每只经嗉囊接种孢子化柔嫩艾美耳球虫卵囊 1 5× 10 5个。试验结果表明 :抗球虫指数 (ACI)由高到低依次为Ⅰ→Ⅵ→Ⅶ→Ⅳ→Ⅴ→Ⅲ→Ⅱ ,增重由大到小依次为Ⅰ→Ⅵ→Ⅶ→Ⅳ→Ⅲ→Ⅴ→Ⅱ ,表明阶段应用西药和中药预防球虫病不仅效果好 ,且能促进雏鸡生长 ,其中以阶段性运用地克珠利和中药组最好     

柔嫩艾美耳球虫对氯嗪苯乙菁和常山酮抗性快速诱导

诱导了柔嫩艾美耳球虫(Eimeriatenella)甘肃株对两种药物-氯嗪苯乙氰和常山酮的抗药性.增加实验动物数和球虫的接种剂量可加快柔嫩艾美耳球虫对氯嗪苯乙氰和常山酮抗药性的产生.每组动物用100只2～3周龄AA肉鸡,每只鸡接种2×10     

so7在大肠杆菌中的表达及表达蛋白和活菌对Eimeria tenella的免疫保护

so7为来源于E.tenella的折光体基因，将T－vector-so7克隆质粒酶切，构建表达载体pThioHis-so7，通过酶切和SDS－page检验，筛选阳性克隆。用表达蛋白或活菌免疫鸡，3周后用柔嫩艾美耳球虫攻毒。结果显示，鸡的相对增重率为71.45%-80.50%，相对卵囊产量为59.27%-92.00%。以口服活菌200μg菌体蛋白组最佳，鸡的相对增重率为80.50%，盲肠相对卵囊数为58.85%。说明so7表达产物能诱导鸡对E.tenella的保护作用。因此，该基因编码抗原可作为基因工程苗的候选之一。     

Partial purification and characterization of acid phosphatase from sporulated oocysts ofEimeria tenella

Summary Acid phosphatase ofEimeria tenella oocysts (Peak II) was purified 77-fold with a recovery of 26% using protamine sulfate precipitation, DEAE-cellulose chromatography and Sephadex G-200 gel filtration. This enzyme occurs in multiple forms as indicated by two peaks which can be separated by DEAE-cellulose chromatography and polyacrylamide gel electrophoresis. The partially purified enzyme has optimal activity at pH 4.5. With p-nitrophenyl phosphate the Km and V_max values for (Peak II) were 25 mM and 1.57 mol/min/mg protein, respectively. The enzyme (Peak II) ist strongly inhibited by Hg⁺⁺, Cu⁺⁺, iodoacetamide, fluoride and molybdate. Tartrate and other divalent metal ions have no effect on enzyme activity. The partially purified Peak II phosphatase is not a glycoprotein as it is not absorbed on concanavalin-A Sepharose and its treatment with bacterial neuraminidase does not alter its elution profile through DEAE cellulose.     

Coccidian parasites of British wild birds

A study of coccidian parasites in British wild birds was undertaken and faecal samples of 5568 birds, representing 141 bird species from 40 families, were examined. Oocysts were found in 18.68% of the samples. Species of the genera Isospora (16.90% infection), Eimeria (1.24% infection), Caryospora (0.48% infection) and Tyzzeria (in three samples) were identified. The overall prevalence of infection did not appear to be influenced by the sex and age of the host. The results are discussed in the light of the general knowledge of coccidian infections.     

基于槲皮苷和桑辛素“双指标”成分检测的桑树寄生质量控制方法研究

为建立一种桑树寄生药材质量控制的方法,采集10批不同产地桑树寄生样品与其寄主桑枝,并采集柳树寄生、肉桂寄生与其寄主柳枝、桂枝作为对照药材,采用高效液相色谱法同时测定桑寄生药材与其寄主样品中槲皮苷与桑辛素的含量,测定条件：色谱柱Waters C₁₈柱（5 μm,4.6×250 mm）,以乙腈（A）-0.1%磷酸（C）为流动相进行梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长为256 nm （槲皮苷）和269 nm （桑辛素）。结果显示：10批桑树寄生样品的槲皮苷含量为1.98-3.11 mg/g,桑辛素含量为0.27-4.27 μg/g;以桑树、柳树和肉桂为寄主的寄生药材均含有槲皮苷,寄主桑枝、柳枝和桂枝均不含槲皮苷,表明槲皮苷属于药材基源广寄生的专属性成分,即广寄生均含有槲皮苷,与寄主无关;桑树寄生与其寄主桑枝均含有桑辛素,柳树寄生、肉桂寄生及其寄主柳枝、桂枝均不含桑辛素,表明桑辛素为桑树寄主的特有成分,桑树寄生药材中的桑辛素为寄主桑树输送而来。本方法可同时测定桑树寄生药材中的槲皮苷与桑辛素“双指标”成分含量,有效鉴定寄主来源,实现对桑树寄生药材的质量控制,方法简便、可行。