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Stahn R Grittner C Zeisig R Karsten U Felix SB Wenzel K 《Cellular and molecular life sciences : CMLS》2001,58(1):141-147
E-selectin, exclusively expressed on activated endothelial cells, is a potential target for site-directed delivery of agents.
We and others have shown that sialyl Lewisx-liposomes (sLex-liposomes) are recognized by E-selectin. We now report an approach employing sLex-liposomes to deliver antisense oligonucleotides (AS-ODNs) directed against the adhesion molecule ICAM-1 to activated vascular
endothelial cells. ICAM-1 expression was analyzed at the protein level by immunofluorescence and a cell surface ELISA, and
at the RNA level by RT-PCR. We have investigated two different AS-ODNs complementary to the 3′ untranslated region and the
AUG translation initiation codon of ICAM-1 mRNA. Both inhibited protein expression, but did not influence the mRNA level,
pointing to a hybridization of AS-ODNs with the mRNA in the cytoplasm. Our results demonstrate the feasibility of a novel
approach for the delivery of agents to activated endothelial cells by glycoliposomes targeted to E-selectin.
Received 16 October 2000; revised 29 November 2000; accepted 29 November 2000 相似文献
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E-选择素与肿瘤转移研究进展 总被引:1,自引:0,他引:1
肿瘤的转移是一个复杂的多步骤过程,它是恶性肿瘤致死性的关键因素.粘附分子与内皮细胞的粘附在此过程中起着重要作用.E-选择素(E-selectin)是粘附分子中的一类,仅表达在内皮细胞上.E-selectin的结构和功能及在肿瘤转移方面的研究表明:静息时,内皮细胞上的E-selectin含量甚微;当内皮细胞受到炎症介质白介素-1(IL-1)、肿瘤坏死因子-alpha(TNF-α)、细菌脂多糖(LPS)等刺激后,E-selectin的表达4 h可达高峰,24 h下降.唾液酸化的Lewis血型抗原(sialyl-Lewisx)是E-selectin的配体,它和E-selectin相互作用共同在肿瘤转移过程中起着重要作用. 相似文献
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选择寡糖Lewis A模拟肽作为特异阻断剂, 探讨通过阻断E-选择素与其配体的结合抑制炎症的可能性. 向一组小鼠腹膜内注射酵母聚糖3 h后, 再于静脉注射表达寡糖Lewis A模拟肽IELLQAR的噬菌体或合成肽IELLQAR, 以空噬菌体或BSA作对照,
观察到实验组腹腔冲洗液中的嗜中性粒细胞显著减少. 为证实该结果, 髓过氧化物酶活性被测定, 酶活性的降低同嗜中性粒细胞的减少平行, 表明注射寡糖Lewis A模拟肽可能通过阻断E-选择素与其配体结合, 防止急性炎症的发生. 相似文献
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MA Yewei ZHOU Xiaoshan ZHAO Qingzheng YANG Jun GAO Xin LI Yanchun LIU Yuying & WANG Zheng . Cancer Institute Peking Union Medical College Chinese Acad-emy of Medical Sciences Beijing China . Beijing Institute of Blood Transfusion Medicine Beijing China Correspondence should be addressed to Zhao Qingzheng 《科学通报(英文版)》2003,(1)
During the DXR process of xenotransplantation, the endothelial cells (EC) are activated, and the expression of NF-kB is immediately up-regulated[1]. NF-kB consisting of p65/p50 heterodimers or homodimers are retained in the cytoplasm by association with IkB (inhibitor of NF-kB) proteins. After stimulation, IkB is degraded, re-leasing NF-kB from these trimeric complexes, and allow-ing NF-kB translocation to the nucleus to play its biology function[2,5]. The promoter and enhancer region… 相似文献
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