桑树萎缩病茎段再生健康植株

用组织培养的方法，探讨了桑树萎缩病茎段再生健康植株的条件．结果显示，严重呈现萎缩症状的桑树茎段在不含任何植物激素的ＭＳ培养基上生长１～３个月，形成的幼苗盆栽后２年中不再显现任何症状．用４，６－二脒基－２－苯基吲哚盐酸盐（ＤＡＰＩ）检测表明组培植株中不再含有类菌原体     

利用DNA荧光探针DAPI检测番茄花粉粒的发育时期

利用荧光物质DAP!对西粉三号番茄不同长度的花蕾进行花粉粒发育时期的鉴定，发现同一朵花中不同花药中花粉粒的发育时期一致，并且番茄花的长度与花粉粒的发育时期之间也有着比较严格的对应关系．利用所得对应关系可以快速、方便的通过测量花的长度来推测花粉粒发育时期．     

冬季桃黄化植原体在树体内分布消长动态的研究

以感染桃黄化病桃树组织作为试验材料,分别利用Nested-PCR技术和DAPI荧光染色技术对冬季桃黄化植原体在树体内的分布进行研究：经Nested-PCR检测黄化桃树叶柄、枝条及根的韧皮部组织均可以扩增得到特异性片段1.2kb,经测序分析为油桃黄化植原体.这表明所检测组织中含有桃黄化植原体;进一步通过DAPI荧光染色观察黄化桃树叶柄、枝条、根及芽胞的韧皮部均可观察到荧光点,结果表明桃黄化植原体在黄化桃树组织中存在于韧皮部组织,冬季在树体内也有分布.     

Mouse Karyotype Obtained by Combining DAPI Staining with Image Analysis

In this study, mitotic metaphase chromosomes in mouse were identified by a new chromosome fluorescence banding technique combining DAPI staining with image analysis. Clear 4＇, 6-diamidino-2-phenylindole （DAPI） multiple bands like （J-hands could be produced in mouse. The Meta- Morph software was then used to generate linescans of pixel intensity for the banded chromosomes from short arm to long arm. These linescans were sufficient not only to identify each individual chromosome but also analyze the physical sites of bands in chromosome. Based on the results, the clear and accurate karyotype of mouse metaphase chromosomes was established. The technique is therefore considered to he a new method for cytological studies of mouse.     

Fluorescence replication banding of frog chromosomes

To identify individual chromosomes of a frog karyotype by their fluorescence banding patterns, chromosomes were stained with actinomycin D and 4,6-diamidino-2-phenylindole (DAPI) after incorporation of BrdU during the late S-phase. The chromosomes of three Rana species which were selected for this study (R. ridibunda, R. lessonae and R. japonica) showed well-defined late replication bands. The fluorescence patterns obtained were the reverse of those produced by a 4Na-EDTA Giemsa-staining technique. Fluorescence patterns of the two water frog species (R. ridibunda and R. lessonae) were similar to each other, except for the different fluorescence of the centromeric heterochromatin, which gave extremely bright signals in R. ridibunda but no signal in R. lessonae. Experiments also showed differences between the fluorescence patterns of R. lessonae chromosome 13 in the Italian and Luxembourgian populations. These results sho w that the fluorescence replication banding using actinomycin D and DAPI is very effective in identifying individual frog chromosomes and detecting their structural changes. Received 7 June 1996; received after revision 23 July 1996; accepted 21 August 1996     

贝类生态综合养殖系统中细菌丰度的变化

2011年4—10月在福建云霄,对以贝类为主的生态综合养殖系统内的藻类池（A池）和贝类池（B池）逐月采样检测,并分析两池水体及B池沉积物中细菌丰度的时空变化规律.结果发现:水体中细菌丰度,B池为1.01×106～4.35×106 cell/mL;A池为1.09×106～6.61×106 cell/mL.A池和B池水体中细菌丰度没有明显的月变化规律,但昼夜变化明显.B池水体中细菌丰度与叶绿素a、总氮、总磷、温度和盐度的关系4—7月呈明显的正相关(r=0.984,n=4);8月细菌丰度急剧下降,因采样期间有连续降雨,细菌丰度变化和降雨可能有一定的关系.A池水体中细菌丰度与叶绿素a呈显著负相关（r=-0.821,n=7）,与温度呈显著负相关（r=-0.830,n=7）,温度不是影响A池细菌增殖的主要因子.B池底泥样品中细菌丰度比水体中的低,主要可能是水交换和养殖贝类活动共同作用的结果.采样期间发现,在阴雨天气时池塘水面会有泡沫漂浮,细菌丰度高于其所在水体达2.25×107 cell/mL,其作用机制有待于进一步研究