排序方式: 共有17条查询结果,搜索用时 15 毫秒
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响叶杨(杨属)叶绿体基因组测序与比较分析 总被引:1,自引:0,他引:1
本研究中,我们对响叶杨的叶绿体基因组进行测序和组装,并将其与杨属其他11个叶绿体基因组进行比较分析.结果表明,响叶杨叶绿体基因组全长158,591bp,其中两个反向重复序列区(IR)长度均为27,667bp,长单拷贝序列区(LSC)和短单拷贝序列区(SSC)长度分别为84,634和18,623bp.通过对杨属12个物种的叶绿体基因组进行比较,只发现了6个相对较大的插入缺失,因此整体而言,杨属的叶绿体基因组结构是高度保守的.系统发育分析结果显示,杨属中12个物种组成了3个具有高支持率的进化支,响叶杨与其他白杨组物种聚为一支,并且与银白杨的亲缘关系最近.本研究基于叶绿体基因组数据揭示了杨属的进化历史,将有助于进一步开展杨属植物基于叶绿体DNA序列数据的群体遗传学及其他分子生态学研究. 相似文献
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利用高离子强度,低pH值缓冲液匀浆细胞的方法,提取小麦叶绿体DNA,并以此为模板,通过聚合酶链反应(PCR)对小麦叶绿体psbA基因进行了特异性扩增。 相似文献
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硒对油菜苗期叶片色素的影响研究 总被引:7,自引:1,他引:7
应用土培与水培相结合的方式,研究不同硒浓度对油菜苗期叶片色素的质量分数的影响,试验表明:在0-24μg/ml的硒浓度范围内叶绿素的质量分数随硒浓度的升高而增加,胡萝卜素的质量分数与施硒浓度没有显著的相关性。 相似文献
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朱天慧 《南开大学学报(自然科学版)》1994,17(3):96-99
本用分离纯化的地中海伞藻叶绿体DNA,以pcos2 EMBL为载体构建了cosmid分子克隆库。并用果蝇的与生物节律控制相关的DNA顺序为探针,从地中海伞藻叶绿体cosmid分子克隆库中筛选到一组含有同源顺序的克隆,经印迹杂交和限制酶分析,这些克隆都含有与果蝇生物节律探针强烈同DNA顺序,且各克隆的限制酶图谱都相互重叠。这一结果表明,在地中海伞藻叶绿体基因组中可能也存在与果蝇相同或基本相同的与生 相似文献
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The chloroplast is the hallmark organelle of plants. It performs photosynthesis and is therefore required for photoautotrophic
plant growth. The chloroplast is the most prominent member of a family of related organelles termed plastids which are ubiquitous
in plant cells. Biogenesis of the chloroplast from undifferentiated proplastids is induced by light. The generally accepted
endosymbiont hypothesis states that chloroplasts have arisen from an internalized cyanobacterial ancestor. Although chloroplasts
have maintained remnants of the ancestral genome (plastome), the vast majority of the genes encoding chloroplast proteins
have been transferred to the nucleus. This poses two major challenges to the plant cell during chloroplast biogenesis: First,
light and developmental signals must be interpreted to coordinately express genetic information contained in two distinct
compartments. This is to ensure supply and stoichiometry of abundant chloroplast components. Second, developing chloroplasts
must efficiently import nuclear encoded and cytosolically synthesized proteins. A subset of proteins, including such encoded
by the plastome, must further be sorted to the thylakoid compartments for assembly into the photosynthetic apparatus.
Received 1 September 2000; received after revision 27 October 2000; accepted 1 November 2000 相似文献
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小麦叶绿体衰老的电镜观察及BA对叶绿体膜系统的影响 总被引:3,自引:0,他引:3
小麦(Triticum aestivum,L.Nondai 18:85021)十天苗第一片真叶切段在暗中诱导衰老。以蒸馏水作对照,用2%乙醇及5μMBA分别处理。比较48小时后叶绿体超微结构变化。BA显著延缓衰老,保持膜系统的完整性,与自由基清除剂乙醇的效果相似。此外,将已衰老48小时后的叶片转入BA溶液又经48小时,发现叶绿体的膜系统恢复到一定程度,接近起始状态。本文对BA保护膜系统的作用进行了讨论。 相似文献
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