脐带囊肿的超声诊断——附9例报告

目的:分析脐带囊肿的声像图特点,提高对该病的认识.方法:回顾性分析9例脐带囊肿的临床及声像图表现.结果:9例囊肿均为单发,其中5例位于胎儿侧,4例位于胎盘侧;均表现为低回声、边界清晰的囊性肿块.结论:超声是观察和诊断脐带囊肿最有价值的影像学方法.     

包囊游仆虫细胞的类中间纤维细胞骨架体系

应用生化分级抽提,并结合DGD包埋 去包埋透射电镜样品制备方法显示,包囊游仆虫营养细胞和休眠细胞中,均存在由直径10 nm左右的单根纤维及单根纤维聚集成的纤维束为结构单元形成的类中间纤维细胞骨架体系.其中,营养细胞的类中间纤维构成的细胞质三维网架,在细胞膜内缘以较密集的纤维网占有了整个表质层,在表质层内缘的细胞质深部纤维形成较松散的网络,网内常见附着有细胞器及一些电子密度颗粒;核纤层位于大核核膜内缘,纤维紧密聚集成网;核骨架纤维网分布比较致密,未见有电子密度颗粒附着.休眠细胞中含有与营养细胞相似的纤维网架结构,但位于细胞内不同层次的纤维网比营养细胞中的同种结构要致密得多,这可能与纤毛虫形成包囊时细胞大范围的收缩有关.并且值得注意的是,在休眠细胞包囊壁的内层壁中也观察到相似于中间纤维的纤维网络,其纤维网均匀和致密地分布在整个包囊壁层中.电泳图谱显示,纤毛虫形成包囊后,保留了营养细胞中的部分蛋白条带,失去了部分条带,新产生了一些特异的条带.结果表明,包囊游仆虫的类中间纤维 核骨架体系,是细胞在营养条件下和休眠状态下都稳定存在的结构,它可能起到比微管类骨架更重要的作用.并且休眠细胞中该体系产生的一些特异蛋白条带,可能是纤毛虫休眠生命活动中的重要蛋白.     

子宫腺肌病囊肿1例临床分析

目的 探讨子宫腺肌病囊肿临床表现、诊断、鉴别诊断及治疗.方法 根据患者病例报告,并查阅相关文献,进一步总结子宫腺肌病囊肿的临床特点.结果 子宫腺肌病囊肿是一类在子宫肌层的较大囊性病变,其内充满子宫内膜异位组织和血液,术中所见及术后病理可明确诊断.结论 子宫腺肌病囊肿的临床表现与子宫腺肌病相似,国内外报道极少,临床误诊率高,临床医生应提高对该病的认识.     

包囊游仆虫营养细胞和休眠细胞的胞器酶细胞化学

为探索毛基体非吸收型休眠包囊的生命活动特征及其胞器的功能,本文应用电镜酶细胞化学方法检测了包囊游仆虫在不同生理状态下的胞器酶反应活性及其定位：（1）在营养细胞中的食物泡膜位置出现紧密排列的腺苷三磷酸酶反应颗粒,但在休眠细胞中酶反应颗粒则聚集在细胞表膜区;（2）营养期细胞中,酸性磷酸酶反应颗粒分布在处于不同消化时期的食物泡或自噬泡内,在休眠期细胞中酶反应颗粒定位在自噬泡周缘;（3）营养细胞的线粒体内膜有稀疏的琥珀酸脱氢酶反应颗粒,休眠细胞中在分散的线粒体内也有稀疏的酶反应颗粒,但反应活性比前者要弱得多.作者由所得结果推测,大部分情况下休眠细胞的酶反应活性较营养细胞的同种反应弱;不同类休眠细胞的生命活动中,其酶的功能和作用部位可能是不一样的;在休眠细胞中也发生着消化和营养利用的过程,也经历着线粒体的功能作用过程.     

Morphogenesis of the division and encystment process of the ciliated protozoan Colpoda minima

We have investigated the main morphological features of the asexual division and encystment process in the ciliate Colpoda minima. It is a soil colpodid ciliate with a quite variable body size. The nuclear apparatus is composed of one ellipsoidal macronucleus and one lenticular micronucleus. The somatic infraciliature is made up of 69–101 kineties, 10–16 of which are postoral. The oral infraciliature is made up of two oral polykineties, the left one consists of 45–60 short oblique rows of kinetosomes whereas the right one is formed by a row of kinetosome pairs and many short rows of kinetosomes disposed without a regular arrangement. Cell division occurs in a division cyst and produces four tomites. Stomatogenesis is merotelokinetal and involves about 16–23 stomatogenic kineties. A macronuclear extrusion process takes place during cell division. The resting cyst is included in the partial-kinetosome-resorbing (PKR) group, and its cyst wall has two layers which are derived from different cystic precursors. During encystment, macronuclear division and chromatin extrusion (in some cases) have been observed. All of these characteristics of the life cycle of C. minima are compared and discussed with respect to those of other colpodid ciliates.     

大豆重迎茬种植与胞囊线虫病发生关系的研究

选择当地有代表性，不同轮作方式的地块，采用定点定期系统调查的方法，对大豆重迎茬种植与胞囊线虫病发生关系进行了研究，结果表明，随着重茬年限的增加，单棉胞囊数显著增加，从而导致相应的大豆生长发育受抑制，大豆根瘤数，侧根数降低，地上部鲜重下降，营养源供应不足，使株荚数，株粒数，百粒重显著减少，导致减产17．5％－40．92％，采用大豆种衣剂26号，1号和拦种剂HF－I号和HF－II号拦种，防效均在75％以上，可在生产中应用。     

原生动物包囊的研究及其意义

包囊的形成在原生动物中是普遍的。本文研究原生动物包囊的形成及脱包囊、包囊的类型、包囊壁的结构以及形成包囊的生物学意义。     

大鹏湾有毒赤潮生物孢囊研究

自１９９年至１９９４年间，对大鹏湾海域９个站位进行了多次采集，对６０份样品进行了分析和观察，从其中发现了４种在世界上引起毒害的赤潮生物孢囊，它们是塔马亚历山大藻，海洋褐胞藻、链状裸甲藻、巴哈马梨甲藻，对这种几种有毒赤潮藻类孢囊的形态特征，生态分布进行了描述，对几种毒害藻大鹏湾引发赤潮的可能性进行了预测。     

长江口及其邻近海域近代沉积物中甲藻孢囊的垂直分布

于2005年4～5月,在长江口外及浙江中南部海域设置5个采样站点,获取了15～40 cm的沉积物柱状样,分层研究了甲藻(dinoflagellates)孢囊的垂直分布.在本研究中共鉴定出46种甲藻孢囊,其中自养型19种,异养型27种.长江口海域复杂的流系特征及环境状况对孢囊分布有明显影响,致使孢囊丰度较低,平均丰度为366.2cysts·g-1,且无明显垂直分布规律.锥状斯氏藻孢囊是最为丰富的自养型甲藻孢囊,平均质量分数介于17.0%～28.9%;异养型甲藻孢囊以原多甲藻属为主,平均质量分数为29.5%～53.6%,其它类型孢囊仅零星分布.有毒藻亚历山大藻孢囊在各站位均有分布,但丰度较低,最高值仅为68.1 cysts·g-1.     

Preliminary animal studies on observation of injured spinal cord with intraoperative ultrasound backscatter microscopy

Many studies have shown that strategies of nerve regeneration and cell-based transplantation are valid based on animal models of spinal cord injury (SCI).To apply these strategies and bridge spinal cord defects,the identification and precise localization of lesions during spinal cord surgery is necessary.The aim of the present experiment was to evaluate the capabilities of ultrasound backscatter microscopy (UBM) in identifying morphologic changes after SCI.After laminectomy,high-resolution ultrasound images of the spinal cord were obtained in one normal and seven spinal cord-injured adult Wistar rats using a UBM system with a 55-MHz center frequency scanner.Comparison between histoanatomic and UBM images was also performed.The results showed that UBM can identify cysts after the experimental SCI is removed in adult rats.In addition,the glial scar formed in secondary injury showed obvious hyperechoic speckle in the UBM image and correlated with the histoanatomic image.UBM has obvious clinical value in nerve regeneration and cell-based transplantation strategies in injured spinal cords.