论峡江地区“烧龙”与“烧畬”的区别和联系

唐宋时期峡江地区“击鼓焚山”的民俗活动是远古时代尚巫之风的残存,其目的是为烧龙祈雨以禳除旱灾;而“烧畬下种”则是自远古“刀耕火种”传承下来的一门农耕技术,但由于“占卜伺雨”的仪式亦使它带上了一些巫风色彩。二者既有明显的区别又有一定的联系。     

Specific anti-tumor effect induced by attenuated Salmonella typhimurium vaccine expressing extracellular region of vascular endothelial growth factor receptor 2

The purposes of this research were to study the stable expression of exogenous gene encoding therapeutic protein in attenuated Salmonella typhimurium, observe the metabolism of oral gene vaccine carried by attenuated Salmonella typhimurium in BALB/c mouse, and investigate the feasibility of prevention and treatment of tumors by the recombinant bacteria. Recombinant plasmid pcDNA3.1 VEGFR2(n1-7) was transformed into competent attenuated Salmonella typhimurium SL3261 to develop oral DNA vaccine SL3261-pcDNA3.1 VEGFR2(n1-7). To observe whether the exogenous gene can be expressed in the recombinant bacteria, PCR was performed to amplify the CMV promoter of the eukaryotic expression vector as the proof of stable expression of exogenous protein; transmission elec- tron microscopy (TEM) was applied to observe the morphology of the recombinant bacteria to confirm that the exogenous gene has no impact on the growth of the bacteria, and then BALB/c mice were immunized with the gene vaccine. After inoculation of the gene vaccine, the recombinant bacteria SL3261 could be detected in the tissues such as small intestine, colon, liver and spleen. And then, mice in each group were challenged with tumor cells. The results of animal experiment showed that tumor growth of the mice in experimental group was inhibited and survival time of immunized mice was prolonged compared with control groups. A higher lymphocyte infiltration in tumors from animals treated with DNA vaccine was observed. Immunohistochemical analysis of tumor samples revealed an en- hanced accumulation of CD8 cytotoxic T lymphocytes, as well as an increase in CD4 cells in the tumors of animals treated with the oral gene vaccine compared to tumors from control group mice. Ultrastructure of the tumor tissue showed that tumor cells in the samples of the immunized mice were well-differentiated. Our research confirmed that the exogenous gene can be stably expressed in the attenuated Salmonella typhimurium and has no impact on the growth of the recombinant bacteria; the exogenous gene can de delivered to the host by attenuated Salmonella typhimurium to produce anti-tumor effect with no obvious cytotoxity to the host. In this study, it is established that attenuated Salmonella typhimurium could be used as a vector for oral gene vaccine, and our study provided a theoretical basis for the body distribution and the metabolism of the recombinant bacteria. This strategy may provide a simple, safe and effective way for the prevention and treatment of tumors.     

鲁西北蜜源植物资源调查

首次报导了鲁西北蜜源植物的种类和花期，对充分利用蜜源资源，合理安排养蜂生产具有重要的指导意义．经充分调查并根据孢粉学知识，分析总结出鲁西北共有主要蜜源植物１５种，辅助蜜源植物１０４种，共计１１９种．     

炼钢─连铸─热轧直接热装一体化计划

从计划角度重点分析了钢铁生产中炼钢、连铸、热轧三者之间联接的较高模式－直接热装轧制计划系统的实现方式，并给出了较为具体的方案。     

根癌农杆菌介导AtNHX1基因转化小麦

利用根癌农杆菌对 3种基因型小麦进行了转化研究 .发现基因型对转化有明显影响 .将小麦品种烟优 36 1、烟 10 3、核生 3号的胚性愈伤组织用含报告基因NPTⅡ和抗盐基因Na H 反向转运AtNHX1的农杆菌菌株pROK2AT GV310 1感染和共培养后 ,用巴龙霉素 5 0 - 15 0mg L筛选抗性愈伤组织及转化植株 .对抗性植株的总DNA用AtNHX1基因的特异引物进行PCR检测 ,目的基因的PCR阳性频率为 1.3(烟 10 3)和 2 .9% (核生 3号 ) .Southern杂交证实外源基因已经整合到小麦的基因组中     

发电厂电气部分课程的工学一体化教学改革探索与实践

详细论述了近年来我们对《发电厂电气部分》课程所做的一系列教学改革与探索，提出了工学一体化教学改革模式，该模式旨在将工程实际贯穿于整个教学活动中，将工程思想、理论学习和工程训练相互融通，使其成为一个动态的整体，采用该模式教学，能够强化学生动手能力和培养创新思维，建立工程思想．     

长春市园林木本地被植物的应用与发展

通过对长春市园林木本地被植物应用的调查研究表明，目前长春市园林绿化常见木本地被植物共有21种：其中，木质藤本地被植物有4种，隶属2科，3属；矮灌木地被植物有10种，隶属5科7属；整形木本地被植物有7种，隶属4科6属。本文介绍了木本地被植物的园林应用及生态型，并提出发展长春市园林地被植物的建议。     

甜菜块根三生构造的形成及发育过程初探

通过“新甜二号”块根三生构造的形成及发育过程观察看出:甜菜块根三生构造的形成始于一对真叶期,从二对真叶到块根糖分增长期前期是块根三生维管束环的主要形成时期。块根内对三生维管束环上的三生维管束束数随块根三生维管束环数的增加而增加,二者之间里显著的直线相关。而且块根内的三生维管束环数及环上的束数都具有极限性。块根最外2—3环上的三生维管束束数虽多,但成熟的较少。块根内各环间距随三生维管束环数的增加而呈动态变化,环间距增长最快在叶丛繁茂中期到块根糖分增长期中后期。生育后期,根内以第三环间距最大,而且根内各环间距的大小与环数之间呈Y=ae~(bx)·x的函数关系。     

火电机组回转式空预器漏风控制系统

对国产回转式空气预热器漏风进行了分析,并提出了回转式空气预热器漏风控制系统的设计思想和方案．