扬子鳄肌肉组织成份测试和营养评价

古医籍早有记载，鳄肉可食。根据江苏省理化测试中心和南京大学现代化分析中心的测定，本文在国内首次发表了鳄肉中所含脂肪、蛋白质、氨基酸、维生素、宏量及微量元素的全部资料，说明鳄肉营养丰富，胜过猪、牛、羊、鸡、鸭肉，并具有海洋生物特有的ＤＨＡ（脑黄金）。对进一步研究怀开发我省特产扬子鳄有重要意义。     

虫草菌最适培养基的正交试验研究

以三因素三水平的正交试验设计培养条件,接种量５％（Ｖ／ｖ）,ｐＨ＝６．５,１２０ｒ／ｍｉｎ,１８℃,７２ｈ,对虫草菌进行液体培养．通过对生长的菌丝体干重的测定及分析,得到该菌种生长最适培养基配方为：葡萄糖,２０ｇ／１０００ｍｌ;ＮＨ４ＮＯ３,１ｇ／１０００ｍｌ;Ｋ２ＨＰＯ４,１ｇ／１０００ｍｌ．并证明碳源为其生长的最重要营养因素     

扬子鳄Sox基因的PCR-SSCP分析

参照人SRY基因HMG－box保守区的序列,设计一对引物,扩增了扬子鳄的Sox基因,并进行了SSCP分析,结果显示扬子鳄Sox基因的扩增片段与人SRY基因扩增片断大小相同,为220bp左右;且雌雄个体间该基因片段的单链迁移率无差异,而与人的有较大差异,本研究为扬子鳄的性别决定机制的探讨及Sox基因的进化保守性分析提供分子资料。     

影响野生扬子鳄生存的环境因素分析

通过对被保护物种生存环境的分析是探知该物种灭绝和种群数量下降的重要手段．2002年9月间，作者在参加南陵县野生扬子鳄栖息地生境的考察工作的基础上，以安徽省南陵县野生扬子鳄生存环境作为对象，对野生扬子鳄的生存的环境因素进行分析评价．结果表明，影响野生扬子鳄生存环境质量的主要因素为食物、隐蔽条件和水体以及人的活动．在安徽省南陵县二个官方指定的保护点中长乐点尽管食物、隐蔽条件和水三大生境基本要素都较为理想，但因为没有足够的栖息地因此这里并不适合野生扬子鳄种群的生存．比较而言，如对楂林点野生扬子鳄的生存环境进行一定的人工修饰，此地应该有着较为合适的生存环境．     

扬子鳄附肢的形态发生

本文在３１例扬子鳄胚胎中观察了附肢的形态发生过程，并与密河鳄附肢的形态发生过程进行和讨论，孵化每天 ，前、后肢芽形成处的组织略隆起；第８天时，前、后肢芽突出呈乳状。前、后肢芽分别在１６、２０天出现近轴部与远轴部结构的区分。前肢掌面和后肢 反在第１６－２４天中形成，第２４－３２天中指间裂逐渐形成。     

扬子鳄DMRT1基因DM保守区的克隆及序列分析

DMRT1基因是DMRT基因家族的一个重要成员,在动物性别决定过程中发挥重要作用.本文参照人DMRT1基因DM保守区及有关文献,设计了一对简并引物,扩增了扬子鳄的DMRT1基因,并对扩增产物进行了亚克隆与测序.结果在雌雄扬子鳄个体中各获得一个预期的基因片段,长度为140bp,序列无性别差异性,命名为扬子鳄AsDMRT1基因.序列同源性分析表明,扬子鳄DMRT1基因DM盒区核苷酸序列与人和鼠相应基因的同源性分别为87%、86%;编码的氨基酸序列与人和鼠相应基因编码序列的同源性分别为95%、97%.充分显示了DMRT1基因在进化上的高度保守性.     

扬子鳄产蛋与天气关系的研究

本文报道了扬子鳄的生殖能力与其在人工饲养下小生境气候之间的关系。５年的研究表明：扬子鳄的产蛋期通常在七月上中旬。这时期的最低平均温度为２５．９°Ｃ，最高平均温度为２８．６°Ｃ，降雨量为６７．３～８１．４ｍｍ。但是当气候变化时会影响扬子鳄的产蛋时间和产蛋窝数     

Testing reintroduction as a conservation strategy for the critically endangered Chinese alligator: Movements and home range of released captive individuals

The Chinese alligator (Alligator sinensis) is considered the most critically endangered crocodilian as a result of the near total loss of its habitat and its extremely small and fragmented wild populations. Plans for population recovery lie mostly with wetland res- toration and the reintroduction of captive-reared animals. We carried out a first-trial release of 3 adult Chinese alligators (1♂, 2♀) into a pond at the Hongxing conservation site, Xuancheng, southern Anhui Province; the animals were radio-tracked from May to October in 2003. We hypothesized that after a period of adaptation, the alligators would establish definable home ranges. Two (1♂, 1♀) of the 3 alligators were monitored for the whole of the tracking period. The male had an annual home-range size of 7.61 hm2, and the female 4.00 hm2. Water temperature and pond water level were two important factors influencing the alligators’ distributions, and daily movements. The radio-tracked alligators had overlapping home ranges, which notably included the one substantial island in the pond; that island is the only known nesting site of the local native wild alligators. Aggressive interactions between the released alligators and native wild alligators were observed during the breeding season around this island. All the three reintroduced alligators survived the winter of 2003 and were alive in the same pond in 2008. We concluded that the Hongxing conservation site provided a suitable habitat for the reintroduced alligators. However, the low water level in the pond resulting from farmland irrigation in August and September can be a substantial threat to the alligators’ survival. Therefore, regu- lations on irrigation in summer and autumn are needed to balance the water needs of the alligators and agriculture.     

扬子鳄SRAP-PCR反应体系的优化及引物筛选

以扬子鳄总DNA为材料,对影响SRAP-PCR的Mg2 、dNTPs、TaqDNA聚合酶、引物浓度等因素进行了优化,分析了TaqDNA聚合酶、样本浓度、Mg2 浓度、dNTPs浓度以及引物浓度对SRAP-PCR扩增结果的影响.筛选出扩增条带清晰、多态性丰富的SRAP引物l3对,建立了稳定的、可重复的扬子鳄SRAP-PCR最佳反应体系及PCR扩增参数.研究结果表明:在30μlSRAP-PCR反应体系中,样本最适宜为1μl(30ng/μl),Mg2 的最适为2μl(2.5mmol/L),dNTPs最适为2μl(2.5mmol/L),单引物的最适均为1μl(10pmol/μl);聚合酶在30μl反应体系中宜加入1U.SRAP-PCR引物筛选及其反应体系的建立,为今后利用SRAP标记技术开展扬子鳄种群的分子生物学研究提供了一个标准化程序和强有力的工具.