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1.
徐传胜 《科技导报(北京)》2021,39(7):120-124
李雅普诺夫是圣彼得堡数学学派的中流砥柱,创立了运动稳定性理论、旋转液团平衡形状理论和微分方程定性理论,探索了概率论中心极限定理和一系列其他深刻数学力学问题,为俄罗斯跻身世界科技强国做出了卓越贡献。通过采撷李雅普诺夫的动人爱情诗篇和唯美科学奋斗目标,展示了其美丽的爱情观和追求卓越的科学精神、人生观和宇宙观。 相似文献
2.
同试管婴儿、克隆人、商业化代孕、干细胞治疗等医疗新技术引发的伦理争议相比,贺建奎基因编辑婴儿事件引发的关注更大、社会反响更为猛烈。探讨贺建奎基因编辑婴儿事件引发国际国内热议的原因,讨论了基因编辑婴儿的伦理、法律和社会蕴含。建议明确界定基因编辑技术的适用对象和范围,建立健全新型医疗技术临床应用的伦理审查和监管体系,提升全社会的伦理意识。 相似文献
3.
用动电位极化和恒电位极化法研究了A3碳钢在不同浓度NaCl的0.5mol/LNaHCO3溶液中亚稳态孔蚀行为。实验发现亚稳态电流波动峰具有快速上升、缓慢下降的特点。亚稳孔出现电位Em 服从正态分布,随着Cl-浓度的提高,Em 值向负方向移动。亚稳孔的峰频变化规律与电位关系不大。恒电位极化时,当电位高于Em而大大低于孔蚀电位Eb 时,电流波动保持一定时间后会最终消失,并产生直径为微米级的小蚀孔。当电位接近孔蚀电位时,一段时间的电流波动后电流往往迅速上升,最终转变为稳定蚀孔. 相似文献
4.
探讨了出口商品结构优化的多元统计分析法及应用,并通过示例说明了该方法在出口商品结构优化中的应用。 相似文献
5.
植物基因环境效应启动子 总被引:5,自引:0,他引:5
胡廷章 《重庆三峡学院学报》2002,18(5):125-128
植物的生长发育受到光照、温度、水分及氧等环境因素的影响,根据对不同环境的响应,植物基因的启动子主要可以分为光效应启动子,温度效应启动子和水效应启动子。在这些启动子中,除了含有基本启动子外,还存在一些特异的顺式调节元件,这些顺式调节元件在特异表达中发挥重要作用。 相似文献
6.
7.
构建T7噬菌体展示禽流感病毒抗原变异性基因片段文库. 首先, 从Gene Bank中查找筛选禽流感病毒抗原变异性基因, 将其截短、 修饰、 简并后得到禽流感病毒抗原变异性基因微阵列. 其次, 将合成的禽流感病毒抗原变异性基因片段文库扩增、 酶切, 链接到双酶切后的T7噬菌体载体基因上, 构成重组噬菌体DNA. 最后, 重组噬菌体DNA经体外包装和扩增, 得到T7噬菌体展示文库, 并进行T7噬菌体展示文库滴度、 重组率和免疫活性测定. 实验结果表明, 从Gene Bank中查找、 筛选、 剪切和修饰共获得96 258条序列构建T7噬菌体展示文库, 原始文库滴度为3.6×107个菌落/mL, 重组率大于90%. 用禽流感病毒H5N1抗体进行捕获, 经聚合酶链式反应(PCR)鉴定, 得到理想目的条带, 证明噬菌体表面展示蛋白具有抗原活性, 可用于禽流感病毒感染患者的快速检测及抗原表位筛选. 相似文献
8.
《科学通报(英文版)》2008,(19)
The purposes of this research were to study the stable expression of exogenous gene encoding therapeutic protein in attenuated Salmonella typhimurium, observe the metabolism of oral gene vaccine carried by attenuated Salmonella typhimurium in BALB/c mouse, and investigate the feasibility of prevention and treatment of tumors by the recombinant bacteria. Recombinant plasmid pcDNA3.1 VEGFR2(n1-7) was transformed into competent attenuated Salmonella typhimurium SL3261 to develop oral DNA vaccine SL3261-pcDNA3.1 VEGFR2(n1-7). To observe whether the exogenous gene can be expressed in the recombinant bacteria, PCR was performed to amplify the CMV promoter of the eukaryotic expression vector as the proof of stable expression of exogenous protein; transmission elec- tron microscopy (TEM) was applied to observe the morphology of the recombinant bacteria to confirm that the exogenous gene has no impact on the growth of the bacteria, and then BALB/c mice were immunized with the gene vaccine. After inoculation of the gene vaccine, the recombinant bacteria SL3261 could be detected in the tissues such as small intestine, colon, liver and spleen. And then, mice in each group were challenged with tumor cells. The results of animal experiment showed that tumor growth of the mice in experimental group was inhibited and survival time of immunized mice was prolonged compared with control groups. A higher lymphocyte infiltration in tumors from animals treated with DNA vaccine was observed. Immunohistochemical analysis of tumor samples revealed an en- hanced accumulation of CD8 cytotoxic T lymphocytes, as well as an increase in CD4 cells in the tumors of animals treated with the oral gene vaccine compared to tumors from control group mice. Ultrastructure of the tumor tissue showed that tumor cells in the samples of the immunized mice were well-differentiated. Our research confirmed that the exogenous gene can be stably expressed in the attenuated Salmonella typhimurium and has no impact on the growth of the recombinant bacteria; the exogenous gene can de delivered to the host by attenuated Salmonella typhimurium to produce anti-tumor effect with no obvious cytotoxity to the host. In this study, it is established that attenuated Salmonella typhimurium could be used as a vector for oral gene vaccine, and our study provided a theoretical basis for the body distribution and the metabolism of the recombinant bacteria. This strategy may provide a simple, safe and effective way for the prevention and treatment of tumors. 相似文献
9.
A/D转换技术及其发展 总被引:1,自引:0,他引:1
为了适应A/D转换发展的要求,A/D转换技术也变得越来越复杂。就A/D转换这一领域所应用的主要转换技术以及各自的特点做一简要的介绍,并由此推断出A/D转换技术的发展趋势。 相似文献
10.
Finding finer functions for partially characterized proteins by protein-protein interaction networks
LI YanHui GUO Zheng MA WenCai YANG Da WANG Dong ZHANG Min ZHU ding ZHONG GuoCai LI YongJin YAO Chen WANG Jing 《科学通报(英文版)》2007,52(24):3363-3370
Based on high-throughput data, numerous algorithms have been designed to find functions of novel proteins. However, the effectiveness of such algorithms is currently limited by some fundamental factors, including (1) the low a-priori probability of novel proteins participating in a detailed function; (2) the huge false data present in high-throughput datasets; (3) the incomplete data coverage of functional classes; (4) the abundant but heterogeneous negative samples for training the algorithms; and (5) the lack of detailed functional knowledge for training algorithms. Here, for partially characterized proteins, we suggest an approach to finding their finer functions based on protein interaction sub-networks or gene expression patterns, defined in function-specific subspaces. The proposed approach can lessen the above-mentioned problems by properly defining the prediction range and functionally filtering the noisy data, and thus can efficiently find proteins’ novel functions. For thousands of yeast and human proteins partially characterized, it is able to reliably find their finer functions (e.g., the translational functions) with more than 90% precision. The predicted finer functions are highly valuable both for guiding the follow-up wet-lab validation and for providing the necessary data for training algorithms to learn other proteins. 相似文献