WindoWs操作系统主引导扇区程序剖析

通过对操作系统启动过程的深入研究，在WINDOWS98 DOS状态下，对当前大容量硬盘主引导扇区的引导过程进行了全面的剖析，导出扩展INT 13H读取大容量硬盘逻辑扇区的方法，这将对大容量硬盘的管理与维护十分有利。     

Bromocriptine-induced removal of endoplasmic membranes from prolactinoma cells

Summary Adenomatous prolactin cells lose 39% of their cytoplasm volume within 7 days after the beginning of bromocriptine treatment. A simultaneous reduction of the rough-surfaced endoplasmic reticulum and the Golgi apparatus occurs. Their membranes are removed by rapid transport along the secretory pathway to the cell surface and to lysosomal destruction.We thank Prof. E. del Pozo, Sandoz Ltd, Basle, for the generous gift of injectable bromocriptine, Prof. E.R. Weibel, Dept. of Anatomy, University of Bern, for his technical advice, Dr U. Helfenstein, Institute of Biostatistics, University of Zürich, for his help in the statistical evaluation of the results and the EMDO-Foundation, Zürich, for financial support.     

La离子注入H13钢氧化层结构分析

研究了Ｌａ离子注入Ｈ１３钢的抗氧化特性，运用扫描电子显微镜（ＳＥＭ），背散射能谱（ＲＢＳ）和俄歇电子能谱（ＡＥＳ）对离子注入生成的表面优化层的结构进行了分析，探讨了抗氧化机理，实验结果说明Ｌａ注入Ｈ１３钢能够得到表面改性的良好效果。     

^13C化学位移与定位效应

以一元取代苯为基本模型，根据取代基对苯环间位仅有诱导效应的实验事实，并假定取代基对苯环间位和对位的诱导效应可视为相等，通过单取代苯１３Ｃ化学位移计算了常见基团的诱导效应（ＲＩ）、共轭效应（ＲＣ）及电子效应（ＲＥ），进而根据电子效应强度（ＲＥ）值定量地表征了定位效应和苯环的活化与钝化     

苏云金芽孢杆菌猝倒亚种Cry1Aa13基因的克隆及序列分析

根据Gen Bank中Cry1A类基因序列设计一对特异性引物，以苏云金芽孢杆茵猝倒亚种质粒DNA为模板，应用PCR扩增技术得到一大小约为3．6kb的DNA片段(Cry1Aa13)．通过引物步行法测定该片段长3598bp，其中开放阅读框(ORF)长3540bp，编码1180个氨基酸，分子量为133．49kD，等电点pI=5．0．序列比较表明该基因与Cry1Aa类基因高度同源(达95％以上)，该基因已在GenBank中登录，登录号为AF510713，并被Btδ-内毒素基因国际命名委员会正式命名为Cry1Aa13。     

基于残差网络的海洋温跃层分析方法

首先, 以世界海洋地图集2013（WOA13）海洋数据为实验数据, 提出将不等距微分法、 垂直梯度法应用于海洋数据预处理、 海洋区域划分和跃层分析中, 并通过对多种神经网络在基于WOA13海洋三维数据二分类实验的性能分析, 选取残差网络作为二分类实验的网络模型, 在三层残差网络模型基础上增加了Dropout保留层以防止过拟合. 其次, 将残差网络模型用于温跃层分析判定, 并针对改进模型进行超参数优化、 残差单元改进、 保留率调整等对比实验. 实验结果表明, 改进的ResNet 26网络对WOA13海洋区域数据的温跃层数据分类有效, 分类准确率超过94%.     

Benthic foraminiferal δ~(13)C minimum events in the southeastern Okhotsk Sea over the last 180 ka

A total of six d13C minimum events,i.e.,VI,V,IV,III,II,and I,were observed via a stable carbon and oxygen isotope analysis of infaunal benthic foraminifera Uvigerina spp.in gravity core OS03-1 in the southeastern Okhotsk Sea over the last 180 ka.These events occurred at112–109,102–90,85–76,57–54,44–40,and 17–10 ka BP.The largest negative excursions reached 2.5%in event V and were greater than 1%in the other events.We proposed that all d13C minimum events were caused by the increase in sea surface water productivity,the weakened formation of Okhotsk Sea intermediate water,and the enhancement of the oxygen minimum zone.The negative excursions were unaffected by methane hydrate destabilization and subsequent methane release based on the results obtained by using archaeal lipid markers.     

软盘特殊格式化及加密技术的研究

通过对软盘结构、物理参数、逻辑参数的分析,给出了一种实现软盘在不破坏原盘数据的基础上,通过使用Int 13H等中断技术,进行重新格式化、扩充软盘容量的方法及加密技术的实现.     

斑马鱼PKR的dsRBM1和dsRBM3重组及体外功能分析

为了重组斑马鱼(Daniorerio)双链RNA依赖的蛋白激酶(DrPKR)的双链RNA结合模体1(dsRBM1)和双链RNA结合模体3(dsRBM3)基因,并对重组基因表达的蛋白进行鉴定和功能分析,设计定向删除引物,应用PCR技术扩增DrPKR的ds RBM1和dsRBM3的基因片段,通过重叠PCR技术将dsRBM1和dsRBM3基因重组在一起得到dsRBM13基因,再将dsRBM13构建到原核表达质粒pET32a上并表达和纯化蛋白;通过表达蛋白的二聚化实验和pull down实验对重组蛋白dsRBM13进行体外功能分析。结果:DrPKR的ds RBM1和dsRBM3重组的蛋白dsRBM13可以进行二聚化和多聚化,且能结合Poly I:C(人工合成的dsRNA)。因此,重组DrPKR蛋白模体dsRBM13仍然具有二聚化和dsRNA结合活性,提示串联了三个dsRBM的Dr PKR激活过程中,串联两个dsRBM对DrPKR激活是必需的,并且在DrPKR结合dsRNA的激活过程中,因dsRNA的长短和空间结构的多样性,含有三个dsRBM的DrPKR可能存在更强和更多的结合方式,因此具有更强的抗病毒免疫反应功能。     

Change in the sedimentary environment of Wanquan River Estuary,Hainan Island,China

Pb geochronology and sediment core profiles of organic carbon, total sulfur and organic carbon isotope (d 13C) values were used to reconstruct the local environ-mental history of the Shamei Lagoon, located in the Wan-quan River Estuary, eastern Hainan Island, China. Totalsulfur and d 13C values decreased upwards in the top 30 cm of a sediment core that spanned the last 200 years of deposi-tion. Total sulfur concentration and d 13C values respectively decreased upward from 1.92% to 0.36%, and -20.63?to -23.64? The C/S ratio in the 19th century and earlier was relatively stable in the range of 0.47—0.80, and there was a positive correlation between organic carbon and total sulfur. Since around 1900 AD, the C/S ratio increased rapidly to a maximum of 3.94, but no simple correlation was found be-tween organic carbon and total sulfur during this more re-cent period. These results indicated that before 1800 AD, the lagoon had a fully marine character, and the location of to-day抯 Wanquan River Estuary was an open embayment.From 1800 to 1900, the salinity of Shamei Lagoon decreased noticeably. The amount of seawater which could enter thelagoon decreased gradually as the Yudai spit grew. Today,seawater scarcely affects the lagoon; it is essentially a fresh-water basin.