受体缺乏改变小鼠脑内组胺含量及昼夜节律

在乙谜麻醉下,分别于明时(8:00 a.m.)及暗时(8:00 p.m.)断头处死野生型及组胺H1R基因敲除型小鼠,迅速取出脑组织并分离出皮层、纹状体、海马、下丘脑、丘脑、中脑及脑干等脑区.这些脑组织被制成匀浆并用HPLC荧光检测法测量其组胺含量.结果显示暗时处死时,H1R基因敲除型小鼠海马、丘脑、中脑及脑干中的组胺含量明显低于野生型小鼠.明时处死时,野生型小鼠各脑区组胺含量均较暗时处死显著降低,但这一变化在H1R基因敲除型小鼠中并未观察到.这些表明作为组胺的功能靶,H1R不仅介导组胺的功能,而且调节大脑中组胺含量与释放的昼夜节律.     

As2O3与不同繁殖条件对果蝇发生量的交互影响

以野生型果蝇、黑檀体果蝇为实验动物，研究了不同浓度As2O3在不同温度、不同空间下对不同品系果蝇发生量的影响。实验共分4个处理浓度、2个培养温度，并重复3次。结果显示不同浓度的As2O3对不同品系的果蝇在不同温度、不同空间条件下的发生量的影响有异，表明不同浓度的As2O3对果蝇的发生量有一定的影响。     

利用蛋白质组学技术研究拟南芥隐色素突变体cry 1的差异表达蛋白

隐色素是一类蓝光受体蛋白,在动植物中调节各种光反应.在拟南芥中隐色素是核蛋白,通过蓝光调节控制下胚轴的伸长,子叶的延展,光周期诱导开花以及生物钟.拟南芥隐色1(CRY1)是一种蓝光受体,主要调节下胚轴的伸长,但CRY1下游的光化学反应仍然不清楚.拟南芥隐色素突变体cry1-304和野生型col-4在蓝光条件下,经过7 d的生长,cry1-304的下胚轴的长度比野生型col-4的下胚轴长.为了弄清楚拟南芥蓝光受体隐色素在调节植物生长发育中的机制,采用双向电泳这种蛋白质组学的方法来分离对比cry1-304和野生型col-4在蛋白水平的表达变化.选取了15个差异蛋白质点,采用MALDI-TOF-TOF-MS进行肽质谱指纹图分析,其中10个蛋白质点得到鉴定和分析,它们包括一些普通酶、氧化还原酶、转录因子、结合蛋白、热休克蛋白等.     

酵母菌呼吸缺陷型和野生型线粒体DNA比较

以酒精酵母，ＩＦＦＩ１３００ 材料，建立了以”蜗牛酶＋机械振荡“复俣自理破碎细胞的方法；先提取并纯化线粒体，后从线粒体中提取线粒体ＤＮＡ的方法，得到了紫外吸收Ａ２６０／２８０为１．８２３，琼脂糖电泳为一条带且分子量大λＤＮＡ的线粒体ＤＮＡ。     

黄化油菜Cr3529的光合特性和叶绿素荧光分析

以野生型油菜3529为对照,研究了黄化突变体油菜Cr3529的光合特性和叶绿素荧光特性.结果显示,幼苗期、初花期、盛花期Cr3529的净光合速率（Pn）均显著低于野生型,但青荚期角果的Pn与野生型的无显著差异;在各个时期,Cr3529胞间CO2浓度(Ci)及气孔导度（Gs）与野生型无显著差异,而其PSⅡ原初光能转化效率（Fv/Fm）均高于野生型;突变体的表观量子效率（AQY）较低,其光饱合点和光补偿点均高于野生型;两者功能叶片的Pn日变化均呈“双峰”曲线,但野生型“午休”程度较突变体弱;从叶绿素荧     

Overexpression of OsRAA1 promotes flowering and hypocotyls elongation in Arabidopsis

Previously, OsRAA1, an AtFPF1 homologue gene, was found to play an important role in modulating rice root development. In the current study, OsRAA1 was overexpressed in Arabidopsis, and the transgenic plants showed early flowering and elongated hypocotyl phenotypes as compared with the wild-type under white-light conditions. The hypocotyls of transgenic lines were twice as long as those of wild-type plants under red-light conditions but were indistinguishable from those of the wild-type under blue and far-red light and darkness. In addition, the phenotypes of AtFPF1 transgenic lines were similar to those of OsRAA1 transgenic lines. These results suggested that OsRAAI/AtFPF1 protein is involved in regulating flowering time and plays an important role in the inhibition of hypocotyl elongation under continuous red light. The functions were preserved during the evolution.     

Kinetics of blue-green light-induced state transition and fluorescence quenching in the wild-type cyanobacterium Synechocystis PCC 6803 and apcD − and apcF − mutants

State transition and blue-green light-induced fluorescence quenching are two short-term processes in cyanobacteria. The details of their kinetics and the relationship between these processes have not been elucidated. In this work, these two processes were studied in the wildtype cyanobacterium Synechocystis PCC 6803 cells as well as in apcD^- and apcF^- mutants by monitoring their time- dependent 77 K fluorescence responses to blue-green light （430-540 nm） at a series of intensities ranging from 20-800 μE m^-2 s^-1. The lowest light intensity to induce fluorescence quenching in wild-type cells was 160 μE m^-2 s^-1 under the selected experimental conditions, while state transition took place at the intensities lower than 160 μE m^-2 s^-1 at a conservative level, but at variable rates. The quenching level increased at intensities higher than 160 μE m^-2 s^-1, reaching the maximum level at intensities equal to or higher than 200 μEm^-2 s^-1. Fluorescence kinetics indicated that both the length of the induction period and time required to reach the maximum level were functions of light intensity. State transitions as well as fluorescence quenching took place in both wildtype and mutant cells, but might involve different mechanisms.     

突变型大肠杆菌二氢叶酸还原酶基因的合成

利用PCR定点突变技术，以野生型大肠杆菌二氢叶酸还原酶基因为模板，获取3种突变型(Cys85Ser．Cysl51Ser，Cys85／151Ser)二氢叶酸还原酶(DHFR)基因．用限制性内切酶BamH Ⅰ与Pst Ⅰ将3种突变基因片段插入到克隆载体pUC18上，进行蓝白筛选，将筛选的克隆进行DNA序列测定．     

芜菁块根汁对果蝇发生量的影响

以野生型果蝇、白眼果蝇为实验动物 ,研究不同浓度芜菁块根汁 (以下简称块根汁 )在不同温度、不同空间下对其发生量的影响。实验共分 3个处理浓度、2个培养温度 (设空白对照组 ) ,重复 3次。结果表明 ,在培养管内 ,3个浓度块根汁均使 2个品系的果蝇在 2 0℃和 2 5℃温度下的发生量增加。在广口瓶内 :2 0℃温度下 3个浓度块根汁均使野生型果蝇的发生量下降 ,而白眼果蝇发生量的影响则为低浓度块根汁使其下降而中、高两浓度却使其增加 ;2 5℃温度下低、中两浓度块根汁使野生型果蝇发生量增加而高浓度却使其下降 ,而白眼果蝇发生量的影响则为低浓度块根汁使其增加而中、高两浓度却使其下降。说明不同浓度的芜菁块根汁对不同品系果蝇在不同温度、不同空间下的发生量的影响是不一样的