Transformation of the salt-tolerant gene of<Emphasis Type="Italic">Avicennia marina</Emphasis> into tobacco plants and cultivation of salt-tolerant lines

Salt-tolerant gene, CSRG1, which was isolated from a kind of salt-tolerant mangroves, Avicennia marina, constructed the transgenic plasmid, pGAM189/CSRG1. CSRG1, GUS, Kmr and Hyg^r could be transferred into tobacco genome by the ameliorated leaf discs method of agro-bacterium-mediate transformation. Thirteen stable resistant lines were obtained when fifty transgenic explants were selected through 50 mg/L hygromycin and 150 mg/L kanamycin. Assessments of PCR amplification, Southern blot analysis and GUS histochemical staining showed that CSRG1 has been integrated into the genome of the eleven transgenic lines (frequency of transformation was 22%). Northern bolt analysis revealed that CSRG1 had expressed in transgenic lines. The assessments of salt-tolerant ability and photosyn-thetic rates indicated that the survival rate of the transgenic lines is 80%—90% and the transgenic lines could increase by 30%—40% in plant height, even when they were cultivated in MS medium containing 2% NaCl and the total seawater (salinity 24). It is supposed that the special physiologic metabolic pathway formed by the products of CSRG1 can really endow the tobacco plants with the high salt-tolerant ability, not only to Na^ stress, but also to the comprehensive stress of various ions.     

硅酸盐去阳离子形成石英脉体的可能性思考

关于地壳中广泛分布的石英脉体的成因，传统的岩浆演化最终产物的观点，长期以来被广泛接受，以地质观察为依据，提出了硅酸盐去阳离子作用、产生石英脉体的新观点。硅的析出是热液交代途径围岩的结果。这种水－岩作用可有水交代、碳酸盐化、氢交代和碱交代等不同方式，并可发生于热水流体运移的全部过程。     

优势互补 战略“双赢”——我院与重庆索特盐化股份有限公司签订产学研合作协议

2005年6月9日，重庆三峡学院与重庆索特盐化股份有限公司就产学研合作举行了签字仪式。院党委书记，院长唐培民教授，副院长胡继明教授，纪委书记陈文昆副教授，物理与电子工程学院副院长谭泽富副教授以及索特盐化股份有限公司党委书记廖正学，副总经理张德胜及有关人员出席签字仪式。签字仪式由学院副院长胡继明教授主持。     

寒武纪大爆发中的磷酸盐化胚胎化石

在扬子地台寒武纪最早期（梅树村期）发现的磷酸盐化保存的后生动物Ｏｌｉｖｏｉｄｅｓ具发育的不同阶段,从胚胎细胞分裂的囊胚期经可能的原肠胚期至胚胎的组织分化期最后直至幼体孵化。后生动物胚胎化石的研究为古生物学研究开辟了一个新领域－化石胚胎学,早期后生动物胚胎学研究对了解寒武纪大爆发中的生命的个体及系统发育有重要意义。     

Proteomic analysis of long-term salinity stress-responsive proteins in <Emphasis Type="Italic">Thellungiella halophila</Emphasis> leaves

Salinity is one of the most severe environmental factors that may impair crop productivity. A proteomic study based on two-dimensional gel electrophoresis is performed in order to analyze the long-term salinity stress response of Thellungiella halophila, an Arabidopsis-related halophyte. Four-week-old seedlings are exposed to long-term salinity treatment. The total crude proteins are extracted from leaf blades, separated by 2-DE, stained with Coomassie Brilliant Blue, and differentially displayed spots are identified by MALDI-TOF MS or QTOF MS/MS. Among 900 protein spots reproducibly detected on each gel, 30 spots exhibit significant change and some of them are identified. The identified proteins include not only some previously characterized stress-responsive proteins such as TIR-NBS-LRR class disease resistance protein, ferritin-1, and pathogenesis-related protein 5, but also some proteins related to energy pathway, metabolism, RNA processing and protein degradation, as well as proteins with unknown functions. The possible functions of these proteins in salinity tolerance of T. halophila are discussed and it is suggested that the long-term salinity tolerance of T. halophila is achieved, at least partly, by enhancing defense system, adjusting energy and metabolic pathway and maintaining RNA structure.     

阴离子组成不同对盐化土壤秸秆分解速率和土壤结构稳定性的影响

甘肃景点灌区分属两个封闭的卷槽盆地，排水不畅，土壤次生盐渍化现象严重。将供试土样施加秸秆后在25℃恒温培养两个月后，对其性质进行测定。结果表明，施加秸秆可以显著降低分散性粘粒含量，提高土壤结构稳定性；Cl^-/SO4^2-比值增加，粘粒分散性降低，土壤结构稳定性增加。     

环保食用菌菌种保存方法

食用菌菌种的保藏方法一般有：冰箱保藏、石蜡封口、沙埋等方法．它们各有特点，但都是通过改变温度和空气中二氧化碳浓度等环境条件来控制它们的生长、降低呼吸及代谢来达到保藏的目的，这些方法在保藏时间长短、被污染程度及对环境的要求方面有很大差异。有的操作技术较复杂，一般人员不易掌握。该方法是在对平菇1015菌株进行驯化的同时，     

数字测井技术分析确定盘龙矿区奥陶系灰岩含水层

合理选用数字测井的方法和参数,利用数字测井资料,结合勘探及抽水试验,综合分析阐述了韩城盘龙矿区峰峰组与马家沟组地层含水层位及富水性。测井、勘探及水文试验工作配合使用,能够相互验证,对区域地质水文作出准确、科学、合理的解释,极大地提高水文地质调查的效率。     

ZrO_2在Na_3AlF_6熔体中的铝热还原

研究了Na_3AlF_6-ZrO_2系相图,还原过程和ZrO_2在此熔体中的电化学行为。结果表明,此体系为简单的二元共晶系,当温度为960℃时,还原反应迅速,浓度可达2.5wt％。     

膜下滴灌改造盐(化)土荒地大田试验总结

通过4年的膜下滴灌改造盐化土荒地大田试验 ,基本掌握了滴灌条件下不同盐分含量土壤盐份、变化规律、脱盐机理、不同灌量对棉花生长发育的影响及其栽培技术要点。