Myocardial dystrophin immunolocalization at sarcolemma and transverse tubules

Using monoclonal antibodies against two different regions of the helical rod part of dystrophin¹, we have localized dystrophin on both plasma membrane and transverse tubules in cardiac muscle of man and several animal species. The staining persisted after experimental ischaemia, and was observed in long-standing heart disease. No immunostaining was seen at the intercalated discs. In skeletal muscle the same two antibodies stained only the plasma membrane.