模糊小脑模型神经网络在多辊冷连轧机轧制力预报模型中的应用

针对宽带钢多辊冷连轧机组特点,为提高轧制力的预报精度,在结合传统轧制压力模型的基础上把模糊算法和神经网络有机结合,设计出基于模糊小脑模型神经网络的多辊冷连轧机轧制力预报模型.通过对传统轧制力模型计算值、小脑模型预报计算值与实测值进行对比分析可知,基于模糊小脑模型神经网络的多辊冷连轧机轧制力预报模型具有较高的计算精度,更适合于多辊轧机在线计算机过程控制的应用,满足现场在线生产的要求,取得良好的板形板厚控制效果.     

Involvement of D-amino acid oxidase in elimination of D-serine in mouse brain

The physiological role of D-amino acid oxidase (EC 1. 4. 3. 3) in mouse brain is described. The presence of D-enantiomers of neutral common amino acids was surveyed in the brain. D-serine was shown to be present at high concentration only in regions where the enzyme activity was low. In normal mice whose D-amino acid oxidase activity was much higher in the cerebellum than in the cerebrum, free D-serine content was apparently lower in the cerebellum than in the cerebrum. In mice of a mutant strain lacking D-amino acid-oxidase activity, the free D-serine level was remarkably high both in the cerebrum and cerebellum. The results suggest that the enzyme is involved in the elimination of free D-serine in the cerebellum.     

Differential distribution of TASK-1, TASK-2 and TASK-3 immunoreactivities in the rat and human cerebellum

In this work, the distributions of some acid-sensitive two-pore-domain K⁺ channels (TASK-1, TASK-2 and TASK-3) were investigated in the rat and human cerebellum. Astrocytes situated in rat cerebellar tissue sections were positive for TASK-2 channels. Purkinje cells were strongly stained and granule cells and astrocytes were moderately positive for TASK-3. Astrocytes isolated from the hippocampus, cerebellum and cochlear nucleus expressed TASK channels in a primary tissue culture. Our results suggest that TASK channel expression may be significant in the endoplasmic reticulum of the astrocytes. The human cerebellum showed weak TASK-2 immunolabelling. The pia mater, astrocytes, Purkinje and granule cells demonstrated strong TASK-1 and TASK-3 positivities. The TASK-3 labelling was stronger in general, but it was particularly intense in the Purkinje cells and pia mater.Received 25 February 2004; received after revision 19 April 2004; accepted 28 April 2004     

Regional distribution of thiamin pyrophosphokinase in rat brain

Summary The highest specific activity of thiamin pyrophosphokinase was found in the cerebellum, and lower activity in cerebral cortex and midbrain. The regional difference in the enzyme activity was similar to that in thiamin content and the influx rate in rat brain, suggesting that the enzyme is involved in the thiamin transport.     

小脑经颅直流电刺激技术提升运动表现的应用

小脑是脑皮层下的一个重要运动调节中枢,它与大脑不同皮层区域在解剖和功能上紧密连接,配合大脑皮层完成机体的运动功能和运动学习。经颅直流电刺激(transcranial direct current stimulation, tDCS)是一种非侵入性的脑刺激技术,通过电极将微弱的电流作用于小脑能有效地提升皮层脊髓兴奋性和调控小脑与大脑皮层间的功能连接。本文系统梳理近20年国内外关于tDCS刺激小脑对提升人类运动表现影响的相关文献,研究结果表明tDCS刺激小脑可以改善人体的运动表现,如姿势控制、运动适应与运动学习、肌肉力量表现等。然而,tDCS刺激小脑的生理机制和刺激强度、刺激时间、刺激时间间隔等参数的选择有待进一步研究。未来的体育科学研究中,如何将tDCS刺激小脑的技术应用于运动训练,帮助运动员突破现有的运动能力仍有待深入探究。     

一种模糊小脑模型神经网络及其仿真研究

在传统小脑模型的基础上,利用其特有的寻址方式,引入模糊逻辑的思想,采用模糊隶属度函数作为接受域函数,提出了一种模糊小脑模型神经网络。重点研究了接受域函数的映射规律、隶属度函数及其参数的选取规律和学习算法。仿真结果表明,该模糊模型具有良好的泛化能力和逼近精度,且可获得连续性强且有解析微分输出的复杂函数。     

模拟5000米低氧大鼠脑内信息物质含量变化初探

利用减压舱模拟海拔５０００米低氧并采用放射免疫的方法，观察了大鼠大脑皮层和小脑内信息物质浓度的变化，并比较了两部位间的含量差异。以西宁地区海拔２２６０米作为对照，发现：高海拔低氧可引起大鼠脑内ｃＡＭＰ含量升高尤以小脑显著。     

广西卵形鲳鲹小脑来源细胞系的建立及特征分析

【目的】卵形鲳鲹(Trachinotus ovatus)是广西重要的海水经济鱼类,但近年来随着养殖规模扩大,各种病害侵染引起的疾病频繁暴发,给广西卵形鲳鲹养殖产业造成重大损失。建立卵形鲳鲹小脑来源的细胞系(Cell line from cerebellum of Trachinotus ovatus,TOCC),将有利于广西卵形鲳鲹养殖中病毒性病害的分离、鉴定和病害的侵染致病分子机理的研究,以及环境污染和变化的动态监测。【方法】采用胰蛋白酶消化法分离得到卵形鲳鲹小脑组织的原代细胞,并进行原代培养。待原代细胞铺满单层后进行传代培养。对稳定传代的TOCC进行18SrDNA基因测序分析,然后进行生长速率分析和染色体分析,并开展病原菌(溶藻弧菌Vibrio alginolyticus)胞外产物对TOCC的毒性分析。【结果】稳定传代的TOCC为成纤维样细胞,18SrDNA基因分析结果显示其来源于卵形鲳鲹;染色体核型分析发现TOCC具有二倍体特征,特征染色体数目为62条;不同培养条件下TOCC细胞的生长速率不同,28℃生长速度最快;病原菌胞外产物对TOCC具有明显的毒性。【结论】本研究成功建立广西卵形鲳鲹小脑组织来源的细胞系(TOCC)。细菌胞外产物对TOCC细胞毒性结果表明,该细胞系在一定范围内能够替代活体检测,实现体外细胞水平对广西海水养殖中病害侵染和环境污染物的快速检测,为养殖病害的有效预防提供技术支持。     

微创术加脑室外引流术治疗小脑巨大血肿临床分析

目的分析YL-1型一次性颅内血肿粉碎穿刺针微创手术加颅脑外引流器行脑室外引流术治疗小脑巨大血肿方法及疗效。方法对我院2004年3月～2009年1月采用微创术加脑室外引流术治疗25例小脑巨大血肿患者临床资料进行分析。结果运用此法治疗可使患者死残率降低、愈合时间缩短。结论正确使用此方法治疗小脑巨大血肿疗效显著,是目前基层医院抢救小脑巨大血肿较好的方法。     

“损伤小脑效应、去大脑僵直”实验方法的改进

为了探索新的、高成功率的损伤小脑效应和去大脑僵直的实验方法,并使2个独立的实验合并进行,笔者采用非麻醉、非开颅的手段,对小鼠小脑一侧进行损伤,并在此基础上,于小鼠人字缝前0.5～1 mm处横断脑干,去除大脑.结果显示小鼠一侧小脑的效应100%显现;绝大多数实验鼠表现出去大脑僵直的效应,实验成功率高达75%.合并2个独立的实验以及使用小鼠代替传统的家兔和猫进行去大脑僵直实验是可行的,其操作简单,重复性好,动物价格低廉,极易于在学生实验中开展.