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The xylosyltransferases I and II (XT-I, XT-II, EC 2.4.2.26) catalyze the transfer of xylose from UDP-xylose to selected serine
residues in the proteoglycan core protein, which is the initial and ratelimiting step in glycosaminoglycan biosynthesis. Both
xylosyltransferases are Golgi-resident enzymes and transfer xylose to similar core proteins acceptors. XT-I and XT-II are
differentially expressed in cell types and tissues, although the reason for the existence of two xylosyltransferase isoforms
in all higher organisms remains elusive. Serum xylosyltransferase activity was found to be a biochemical marker for the assessment
of disease activity in systemic sclerosis and for the diagnosis of fibrotic remodeling processes. Furthermore, sequence variations
in the XT-I and XT-II coding genes were identified as risk factors for diabetic nephropathy, osteoarthritis or pseudoxanthoma
elasticum. These findings point to the important role of the xylosyltransferases as disease modifiers in pathologies which
are characterized by an altered proteoglycan metabolism. The present review discusses recent advances in mammalian xylosyltransferases
and the impact of xylosyltransferases in proteoglycan-associated diseases.
Received 9 February 2007; accepted 5 March 2007 相似文献
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Wilson IB 《Cellular and molecular life sciences : CMLS》2004,61(7-8):794-809
In a journey lasting 40 years from the first reports on its activity in the 1960s to its purification and the cloning of relevant complementary DNAs, peptide O-xylosyltransferase has finally arrived at the same point as many other enzymes. This enzyme, whose systematic name is UDP--D-xylose:proteoglycan core protein -D-xylosyltransferase (EC 2.4.2.26), catalyses the first step in the biosynthesis of chondroitin, dermatan and heparan sulphates in the endoplasmic reticulum and/or the cis-Golgi cisternae. Analyses of their primary structure show that peptide O-xylosyltransferases are members of glycosyltransferase family 14 and so are homologous to 1,6-N-acetylglucosaminyltransferases involved in O-glycan and poly-N-acetyllactosamine branching. Furthermore, vertebrates appear to have two rather similar genes encoding xylosyltransferase I and xylosyltransferase II, but enzymatic activity was only detected for a recombinant form of the first isoform. On the other hand, Caenorhabditis and Drosophila have each only one peptide O-xylosyltransferase gene. In the worm sqv-6 mutant, a mutation of the xylosyltransferase gene is associated with defective vulval morphogenesis, indicative of the importance of the glycosaminoglycan chains of proteoglycans in animal development. There remain, however, open questions, for instance, on the enzymes intracellular localisation and structure-function relationships.Received 11 July 2003; received after revision 4 September 2003; accepted 24 September 2003 相似文献
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