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排序方式: 共有154条查询结果,搜索用时 31 毫秒
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X. Wang C. A. Lessman D. B. Taylor T. K. Gartner 《Cellular and molecular life sciences : CMLS》1995,51(11):1097-1102
Gastrulation is characterized by dramatic cell migration which is thought to require the interaction of cell adhesion molecules with extracellular molecules. We have tested two novel peptides, a fibronectin peptide and a fibronectin receptor peptide, for their effects on gastrulation of the leopard frogRana pipiens. The fibronectin peptide DRVPHSRNSIT corresponds to residues 1373–1383 of the cell-binding domain of fibronectin; the receptor peptide DLYYLMDL corresponds to residues 124–131 of 1 subunit of a variety of integrins including 51. Either of these peptides significantly inhibited gastrulation after being microinjected into mid-blastulae. These results indicate that these sequences may correspond to the ligand/receptor interaction sites of fibronectin and its receptor(s). 相似文献
3.
重组人巨细胞病毒(HCMV)嵌合肽在毕赤酵母中的表达 总被引:2,自引:1,他引:2
目的:在巴期德毕赤酵母中表达人巨细胞病毒gp52C末端和pp150C末端串联片段的嵌合肽,方法:用SacI和BglⅡ分别酶切CMVp-pPIC9K重组质粒,电打孔法导入毕赤酵母GS115后,在缺组氨酸的MD板上筛选出转化子,然后根据甲醇利用快速型(Mut^ )和甲醇利用缓慢型(Mut^s)菌株的不同生长特点,筛选出Mut^ 和Mut^s型转化子,用PCR法进一步鉴定阳性克隆,分别用甲醇诱导Mut^ 和Mut^s型转化子表达目的蛋白4d,取培养产物冻干浓缩,进行SDS-PAGE和Western blotting,筛选出能特异表达目的的蛋白的菌株,分析蛋白的含量及纯度。结果:重组人巨细胞病毒可在甲醇利用快速型毕赤酵母中有效表达,其表达量约占培养上清分泌蛋白的76.5%,结论:重组人巨细胞病毒(HCMV)嵌合肽可在真核细胞毕赤酵母中成功表达。 相似文献
4.
牡蛎低分子活性肽对人肺腺癌A549细胞形态与超微结构变化的影响 总被引:4,自引:0,他引:4
以牡蛎(Saccostrea cucullata)体内分离提取出的牡蛎天然低分子活性多肽(BPO-L,Bioactive Peptides of Oyster in Peak L)为效应物,观察研究BPO-L对人肺腺癌细胞形态和超微结构的影响.光镜观察显示经0.1mg/mL BPO-L处理后的A549细胞体积增大,趋于扁平铺展状态,细胞核质比例变小,核仁数量减少.透射电镜观察显示经0.1mg/mL BPO-L处理后的A549细胞核形态较规则,细胞核内异染色质减少,常染色质增多;细胞质内线粒体数量增多,结构较为一致,高尔基体呈较为典型发达状态,并出现粗面内质网增多和多聚核糖体减少等变化.结果表明,BPO-L能有效改变人肺腺癌细胞的恶性形态与超微结构特征,对肺癌细胞具有一定的诱导分化作用. 相似文献
5.
玉米蛋白生物活性肽的开发 总被引:14,自引:0,他引:14
金英姿 《新疆师范大学学报(自然科学版)》2004,23(2):40-42
玉米蛋白含有丰富的蛋白质和独特的氨基酸组成,通过酶法改性可得到大量的生物活性肽。本介绍了玉米蛋白酶解物中的生物活性肽及其特殊的生理功能,利用低价值的玉米蛋白生产高附加值的生物活性肽具有原料来源丰富、成本低、安全性好等特点,可以充分利用玉米资源,大大提高玉米加工企业的经济效益和社会效益,应用前景十分广阔。 相似文献
6.
7.
根据获得的16条ELP序列及相变温度的数据,利用伪氨基酸组成方法提取其序列特征值.将伪氨基酸组成中的相关系数部分作为类弹性蛋白的特征向量,从类弹性蛋白序列出发,利用最小中位方差回归,找出与其序列相关系数的最佳阶数.运用均匀设计法,分别对支持向量机与BP神经网络参数进行优化.结果表明:BP神经网络获得的预测模型最佳,相变... 相似文献
8.
Human skin is permanently exposed to microorganisms, but rarely infected. One reason for this natural resistance might be
the existence of a ‘chemical barrier’ consisting in constitutively and inducibly produced antimicrobial peptides and proteins
(AMPs). Many of these AMPs can be induced in vitro by proinflammatory cytokines or bacteria. Apart from being expressed in vivo in inflammatory lesions, some AMPs are also focally expressed in skin in the absence of inflammation. This suggests that
non-inflammatory stimuli of endogenous and/or exogenous origin can also stimulate AMP synthesis without inflammation. Such
mediators might be ideal ‘immune stimulants’ to induce only the innate antimicrobial skin effector molecules without causing
inflammation.
Received 9 August 2005; received after revision 21 October 2005; accepted 16 November 2005 相似文献
9.
Lubelski J Rink R Khusainov R Moll GN Kuipers OP 《Cellular and molecular life sciences : CMLS》2008,65(3):455-476
This review discusses the state-of-the-art in molecular research on the most prominent and widely applied lantibiotic, i.e., nisin. The developments in understanding its complex biosynthesis and mode of action are highlighted. Moreover, novel applications
arising from engineering either nisin itself, or from the construction of totally novel dehydrated and/or lanthionine-containing
peptides with desired bioactivities are described. Several challenges still exist in understanding the immunity system and
the unique multiple reactions occurring on a single substrate molecule, carried out by the dehydratase NisB and the cyclization
enzyme NisC. The recent elucidation of the 3-D structure of NisC forms the exciting beginning of further 3-D-structure determinations
of the other biosynthetic enzymes, transporters and immunity proteins. Advances in achieving in vitro activities of lanthionine-forming enzymes will greatly enhance our understanding of the molecular characteristics of the
biosynthesis process, opening up new avenues for developing unique and novel biocatalytic processes.
Received 9 April 2007; received after revision 31 August 2007; accepted 28 September 2007 相似文献
10.
Zinc binding to peptide analogs of the structural zinc site in alcohol dehydrogenase: Implications for an entatic state 总被引:1,自引:0,他引:1
Bergman T Zhang K Palmberg C Jörnvall H Auld DS 《Cellular and molecular life sciences : CMLS》2008,65(24):4019-4027
Zinc binding to the peptide replica and analogs to residues 93–115 of horse liver alcohol dehydrogenase (ADH) was examined
by competition of the peptides and the chromophoric chelator 4-(2- pyridylazo)resorcinol for zinc and X-ray absorption fine
structure analysis of the zinc ligands. In the enzyme, zinc is coordinated by four Cys residues. In the peptide replica, zinc
is bound to three Cys and one His residue. A four-Cys zinc coordination is observed only when His is removed, leading to increased
zinc stability. ADH crystal structures reveal that the ε-amino group of the conserved residue Lys323 is within H-bond distance
of the backbone amide oxygens of residues 103, 105 and 108, likely stabilizing the zinc coordination in the enzyme. The peptide
data thus indicate structural strain and increased energy in the zinc-binding site in the protein, characteristic of an entatic
state, implying a functional nature for this zinc site.
Received 3 July 2008; received after revision 11 August 2008; accepted 1 September 2008 相似文献