Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination

The long-arm and short-arm genes of fibroin light chain (L-chain) of silkworm, Bombyx Mori L., and the gene of human acidic fibroblast growth factor were cloned respectively and subsequently inserted into a transfer vector pVL 1392 used as a tool to target the L-chain region of the silkworm genome. Genomic DNA from their offsprings was extracted and the expected targeting was detected using polymerase chain reaction and DNA sequencing, as well as protein analysis. The results showed that positive events occurred and that the FGF gene was integrated into the L-chain locus through homologous recombination.     

电视广告中人物形象探析

广告人物形象蕴涵着广告的诸多因素,电视广告中的人物形象是商品与消费者之间的媒介物,是媒介方式具有语言传递和人性丰富情感交织的物态化显现。由于商业艺术本身具有的功能性,广告人物形象是有别于纯艺术人物形象的,只有研究和遵循广告人物形象塑造的规律和特点,规避人物形象塑造的误区,才能充分发挥广告人物形象在电视广告中的积极与能动作用。     

历书作为媒介物与“睹历思明”——当尊周思明的朝鲜人面对历书

朝鲜被清朝征服后,仍长期以明朝为正统,形成了强烈的尊周思明文化心态。文章讨论了有清一代朝鲜君臣对于不同历书的复杂情感,以及特定的处理方式：明朝先前所颁之《大统历》历书,勾起了朝鲜君臣关于明朝以及皇帝恩情的历史记忆,他们将旧历书视为故国遗物,对其倍加珍视与尊崇;由于朝鲜通行的历书——清朝所颁《时宪历》以及观象监所制之历——刊载有清朝年号,士大夫们对其相当厌憎,甚至对其加以改造。朝鲜人的“睹历思明”现象,有助于今人反思古代东亚世界中,历书作为媒介物在不同情境下的性质变化。