pH值对应用mATP酶法进行梭外肌纤维分型的影响

目的 探讨酸性和碱性预孵育处理，对应用mATP酶法进行梭外肌纤维分型的影响。方法 mATP酶法。结果 ①用pH4．3的预孵育液孵育后，梭外肌纤维可分为Ⅰ，ⅡA，ⅡAB及ⅡB型；pH4．6预孵育后，梭外肌纤维可分为Ⅰ，ⅡA和ⅡB型；pH9．4和pH10．4预孵育液条件下，梭外肌纤维均可分为Ⅰ型和Ⅱ型肌纤维，且Ⅰ型肌纤维的比例略低于酸法。②预孵育液和作用液的pH值随时间的延长均有下降的趋势。结论 用mATP酶法研究梭外肌纤维的分型时应当说明并严格把握预孵育液的pH值。     

科学搜搜

汽车漂着开 21岁的中国学生能设计出啥样的车？看，就是它——大众Aqua。虽然它的身材像个扁平的比目鱼．但是身手非常矫腱，无论是在海上、冰面，或者坑洼不平的路面，它都能开得四平八稳。     

十种奇异透明动物

据国外媒体报道,透明或接近透明,并不意味着动物没有什么可隐藏。相反,没有颜色可以帮助它们躲开天敌。透明性还有助于生物保存珍贵的资源,这个好处谁都能看得到。1.透明乌贼属于酸浆鱿科(Cranchiidae)的乌贼,大约有60多种。透明乌贼通常又     

Isolation and characterization of a new species of Vibrio-Vibrio olivaceus sp.nov. from diseased flounder （Paralichthys olivaceus temminck et schlegel）

One kind of pathogenic bacteria was isolated from diseased (or dead) flounder (Paralichthys olivaceus L.) which expressed bacterial septicaemia. The phenotyic information of the 10 pure cultures was studied extensively, including morphological characteristics, colony characteristics in different media, physiological and biochemical characteristics, and the mol%G C ratio of the DNA for representative strain. The results showed that the isolates belonged to a new species of Vibrio. In addition, the representative strains have been re-checked by China Center for Type Culture Collection (CCTCC), the results were the same as ours, the examined strains were also regarded as a new species of Vibrio, and designated as Vibrio olivaceus sp.nov. by its isolated fish (Paralichthys olivaceus) based on its biological properties following Rules of International Code of Nomenclature of Bacteria. In the same time, we conducted studies on the serum homology of isolates, pathogenicity of isolates by experimental infection. The results showed that all the isolates were of serologic similarity and the isolates have strong pathogenicity to flounder. Inspection to pure cultures and isolates from liver of moribund fish from infection experiment by fluorescent antibody technique showed the feasibility of fluorescent antibody technique as an auxiliary method of examination.     

PH值对应用肌球蛋白 ATP酶法进行梭内肌纤维分型的影响

探讨酸性和碱性预孵育处理，对应用肌球蛋白ATP酶法进行梭内肌纤维分型的影响。采用肌球蛋白ATP酶法。PH4．3和PH4．6预孵育液孵育后，梭内肌纤维中的核袋纤维染色呈阳性，核链纤维染色相对较浅；PH9．4预孵育后，核袋与核链纤维均呈阳性或强阳性；PH10．4预孵育后，核袋1纤维呈阴性，核袋2纤维呈阳性，核链纤维呈强阳性。结论是：用mATP酶法研究梭内肌纤维的分型时，预孵育液的PH值对分型结果有显著影响，应当说明并严格把握预孵育液的PH值。     

国外期刊亮点

正破译首个比目鱼基因组中国水产科学研究院黄海水产研究所SSoonngglliinn CChheenn等破译了迄今为止世界上首个比目鱼基因组——半滑舌鳎全基因组精细图谱,这标志着鲆鲽鱼类养殖研究进入基因组时代。研究成果发表于3月出版的Nature Genetics。研究发现,普通养殖群体中的生理雌鱼比例仅为10%~30%;伪雄鱼Z染色体上的性别调控基因的甲基化可以遗传给后代;半滑舌鳎伪雄鱼后代更容易变成伪雄鱼;伪雄鱼W精子难以成活是导致ZW雌鱼和ZW伪雄鱼交配后代中没有WW超雌鱼的奥秘,这为半滑舌鳎性别控制和高雌性苗种研制提供了理论依据和技术手段。研究还发现半滑舌鳎变态发育前后差异基因富集于底栖适应性相关性状,筛选到可能与变态发育相关的15个正选择基因;半滑舌鳎表现出对底栖生活的适应性和明显的视觉退化现象;揭示了半滑舌鳎底栖生活阶段视觉退化后可能通过强大的侧线器官以及特异的感觉器官-乳头状突起进行弥补的分     

当归对吊尾大鼠比目鱼肌收缩功能的影响

探讨当归对吊尾14天大鼠比目鱼肌(SOL)收缩功能的影响及可能的作用机制。以尾部悬吊法建立大鼠废用性肌萎缩模型,将24只雌性SD大鼠分为正常组(CON),吊尾组(TS)和灌胃当归组(TS+Ang)。用PowerLab系统测定比目鱼肌等长收缩与强直收缩最大张力;定磷法测定Ca2+-ATP酶活性。结果显示:1 TS组肌肉湿重(MWW)和肌肉湿重/体重(MWW/BW)比CON组显著降低,TS+Ang组肌肉湿重升高了17%(P0.05),而肌肉湿重/体重不具统计学意义;2 TS组等长收缩最大张力(Pt)和强直收缩最大张力(Po)比CON组均显著降低;与TS组相比,TS+Ang组Pt值和Po值分别增加91%和49%(P0.01);3与CON组相比,TS组等长收缩最大张力达到一半的时间(TP50),达到等长收缩最大张力的时间(TPT)以及从最大张力峰值到舒张50%(RT50)和75%(RT75)的时间均显著缩短;与TS组相比,灌胃当归组分别增加109%,51%,57%和76%(P0.01);4吊尾组肌浆网Ca2+-ATP酶活性显著升高,灌胃当归后,Ca2+-ATP酶活性降低10%(P0.05)。结果说明:当归能有效缓解吊尾造成的肌肉收缩张力的下降和时程的缩短,当归对抗吊尾引起的比目鱼肌Ca2+-ATP酶活性的升高可能是其抗废用性肌萎缩的重要机制之一。     

Isolation and Characterization of a New Subspecies of Aeromonas salmonicida （ Aeromonas salmonicida subsp, flounderacida subsp. nov. ） from Stone Flounder（ Kareius bicoloratus L. ）

Biological properties were studied to appropriate pathogenic bacteria which were isolated from diseased (or dead) stone flounder (Kareius bicoloratus L. ) which expressed bacterial septicaemia, including morphological characteristics, colony characteristics, physiological and biochemical characteristics and serum homology of isolates, the results showed that the isolates belonged to a new subspecies of A. salmonicida. In addition, the representative strains have been re-checked and detected the mol% G C ratio of the DNA by China Center for Type Culture Collection (CCTCC), the examined strains were also regarded as a new subspecies of A. salmonicida, and designated as Aeromonas salmonicida subsp, flounderac/da subsp, nov. by its isolated fish (Kareius bicoloratus ). Molecular identification of analysis of the nucleotide sequence of the 16S rRNA gene were applied, the results showed high similarity (99%) with the 16S rRNA gene of Aeromonas salmonicida from GenBank database. Cluster analysis of phylogenetic tree revealed that the representative strain formed separately bootstrap-supported cluster.     

2．45米长大鱼

今年70岁的德国垂钓者冈瑟·汉塞尔（Gunther Hansel）在冰岛西峡湾之旅中，用了两个多小时的时间征服了一条超大比目鱼，同时还打破了一项纪录。     

开怀大猜

1.武侠小说中将能改变自己形态、容貌的技艺称为易容术,而东南亚的模仿章鱼可谓是动物界的易容高手。它至少可以模仿15种动物,包括海