用柱后衍生荧光测定法检测食品中土霉素和四环素残留

应用高效液相色谱(HPLC)对动物性食品中土霉素(OTC)和四环素(TC)进行测定,样品经含乙二胺四乙酸二钠的丁二酸钠缓冲液除蛋白后,由SPE-C18小柱提纯净化,经ODS-C18柱分离后,用8%氧氯化锆溶液柱后衍生,与锆离子的螯合物在激发波长(Ex)406nm和发射波长(Em)515 m处检测.实验优化了锆试剂的浓度和柱后衍生的条件,提高了检出的灵敏度,土霉素和四环素的回收率分别为89.59%和92.59%,变异系数(CV)分别为4.98%和6.74%.土霉素和四环素的最低检出浓度(信噪比51)分别为3μg/L和5 μg/L,线性范围为0.01～10g/L,土霉素和四环素的相关系数分别为0.999 9和1.000 0.     

诺氟沙星——铽（Ⅲ）与核酸的相互作用研究及其应用

研究了诺氟沙星—铽(Tb^3 )络合物与核酸(DNA)的相互作用，据此建立了核酸的测定方法，讨论了诺氟沙星—铽与DNA分子之间的结合方式。     

嗜热蓝藻鞭枝藻藻胆体的分离及其光谱特性的研究

从嗜热蓝藻鞭枝藻（Ｍａｓｔｉｇｏｃｌａｄｕｓｌａｍｉｎｏｓｕｓ）中分离出藻胆体，并对其光谱特性进行了研究．完整藻胆体的室温荧光峰位于６７３ｕｍ，７７Ｋ荧光发射光谱中只有一个峰，位于６８５ｕｍ，解离的藻胆体的７７Ｋ荧光峰位于６８３ｎｍ，并在６６６ｎｍ和６５０ｎｍ处形成小的荧光发射峰．严重解离的藻胆体室温荧光峰位于６４５ｕｍ，７７Ｋ荧光峰位于６４４ｕｍ，６８３ｕｍ荧光显著下降，成为小峰．完整藻胆体与解离的及严重解离的藻胆体的吸收光谱没有显著差别，吸收峰位于６２２ｕｍ，在５８０ｕｍ处有一吸收肩．     

Spectroscopic study on the photophysical properties of chlorine substituted tetraphenylporphyrin-histidine and its zinc(Ⅱ)complexes

As the structural analogues of (bacterio)chlorophylls in photosynthetic organisms, porphyrins play an important role in building up artificial photosynthetic systems[13]. In natural photosynthetic pigment-protein complexes,certain amino acid residuals modulate the reactions ofcharge transfer and energy transfer via coordination andother types of interactions with chlorophyll molecules.For instance, in higher-plant photosynthetic reaction cen-ter PSⅡ, the oxidization-reduction system consistin…     

氢化物发生-原子荧光法测定菊花和大云中的铅

应用AFS—2202a型双道原子荧光分光光度计和断续流动的氢化物发生技术，采用HNO3-H2O2酸性体系和高压釜消解样品，对样品中的铅含量进行了测定。同时，研究了光电管负高压、灯电流、屏蔽气和载气流量、载流的选择、酸度等对实验的影响。在选定实验条件下，铅的检出限为0．08μg／L，标准物质桃叶(GBW08501)的验证结果与推荐值一致，相对标准偏差为1．9％。该方法简便、快速、灵敏，对于实际中药样品的测定得到了满意的结果。     

The concentration quenching characteristics of ~5D_3-~7F_J and ~5D_4-~7F_J (J=0―6) transitions of Tb~(3 ) in YBO_3:Tb~(3 ) phosphor

The photoluminescence quenching behaviors of 5D3-7FJ and 5D4-7FJ (J = 0―6) transitions of Tb3 in YBO3:Tb under 130―290 nm excitation were systematically investigated. The results revealed that the quenching concentrations of both 5D3-7FJ and 5D4-7FJ transitions of Tb3 in YBO3:Tb were mainly de-pendent on excitation wavelength. Particularly,the quenching concentrations of 5D4-7FJ transitions of Tb3 under 130―290 nm excitation were correlated with excitation bands of YBO3:Tb. The quenching concentrations of 5D3-7FJ transitions remained at low concentration (2%) under 186―290 nm excitation and then increased gradually with energy of incoming excitation photon when excited at 130―186 nm. This dependence should be involved in their excitation mechanisms and quenching pathway in particular excitation region.     

W_(18)Cr_4V钢真空油淬白亮层、熔化层形成原因的试验研究

W_(18)Cr_4V钢真空加热油淬后,表面出现白亮层和熔化层等异常现象。本文利用金相显微镜,显微硬度计、扫描电子显微镜和电子探针等手段对白亮层和熔化层进行了分析研究。试验表明,白亮层、熔化层均是真空油淬增碳的结果。本文对白亮层、溶化层形成原因进行了比较深入的分析探讨提出了产生白亮层必须同时具备的三个条件。     

Storage of irradiated human blood; a source of error in quantitative chromosome analysis

Summary Human whole blood was irradiated with 2.5 Gy of 220 kVp X-rays and stored before culture with 9.7 M BrdU and 19.4 or 38.7 M BrdU for 0, 24, 48 and 72 h. The frequency of dicentrics and ring chromosomes was determined in cells staining as first division (M1) metaphases with the fluorescence plus Giemsa technique. Storage had no influence on the observed aberration yields in 44 h cultures containing 9.7 M BrdU. In 66 h cultures at 19.4 M BrdU the observed yields after 2 and 3 days' storage were significantly lower as compared to cultures from fresh blood. No storage effect was revealed in 66 h cultures containing 38.7 M BrdU. In cases where cytogenetic radiation dosimetry has to be carried out using blood samples which have been in transit for 2–3 days, the findings are of relevance for a correct determination of the chromosome damage in M1 cells.     

细胞内游离钙的荧光测定

由于细胞内游离钙浓度是重要的生理指标之一,所以重点在测定细胞内游离钙的技术要求、影响测定的重要因素、钙的荧光试剂以及新进展四个方面叙述。实验证明Ｗａｚｏ－１试剂对细胞无毒性,与钙反应迅速、灵敏及选择性高,细胞内游离钙的荧光测定快速准确。但是,荧光试剂的合成路线较长,还需要改进。