卡波氏肉瘤相关疱疹病毒ORF50结合蛋白的筛选

应用酵母双杂交系统,以包含RTA氮端530个氨基酸的片段插入载体pGBKT7作为诱铒,在人脾脏cDNA文库中,筛选得到4个阳性AD/文库质粒,并用酵母双杂交实验验证了阳性AD/文库质粒与RTA的相互作用.将阳性AD/文库质粒测序并对测序结果做BLAST分析,发现它们分别是:TLE2、RBP-Jk、ZNF-12和WHSC1.     

Kaposi＇s sarcoma-associated herpesvirus. Towards better understanding of its latency and pathogenesis

Kaposi＇s sarcoma-associated herpesvirus （KSHV） is a double stranded DNA virus. It was found to be related to Kaposi＇s sarcoma （KS）, primary effusion lymphoma （PEL） and multicentric Castleman＇s disease （MCD）, which cause severe illness in AIDS patients. As a member of human herpesvirus family, KSHV displays two distinct phases in its life cycle, the default latent and lytic replication phase. Following primary infection, the virus can quickly establish latent infection in the host. However, it is still not fully understood up to date how KSHV establishes and maintains viral latency in the host cells. KSHV mainly infects endothelial cells in the host, promoting proliferation and angiogenesis. Abundant angiogenesis is the key feature of KS and is the critical factor for KS progression. The mechanism of KSHV mediated pathogenesis is also largely unknown. In this review, we summarize the recent progress in the mecha- nisms of KSHV latency and pathogenesis, with particular views from our work.     

新疆某地维吾尔族男性吸毒者KSHV血清流行病学初步分析

为进一步研究HIV-KSHV感染相关疾病提供基础资料,利用ELISA法检测KSHV潜伏期LANA1、溶解期ORF65、K8.1抗原抗体,采用χ2检验分析有关数据,分析了新疆某地维吾尔族男性吸毒人群的KSHV血清流行病学的特点。吸毒人群主要以注射吸毒者为主(184/199,92.5%),在199份吸毒者血清中共检测到KSHV阳性血清60份,KSHV血清阳性率是30.6%;χ2检验分析显示,年龄、职业、文化程度、婚姻状况、HIV-1血清状态、吸毒方式以及吸毒年限均与KSHV血清阳性率无关,该吸毒人群中KSHV感染率较高,表明KSHV普遍存在于新疆某地维族男性吸毒人群。     

卡波氏肉瘤相关疱疹病毒vGPCR具有巯基化酪氨酸的氨基端的表达和纯化

卡波氏肉瘤相关疱疹病毒(KSHV)编码的G-蛋白偶联受体(vGPCR)基因是一种癌基因,在KSHV致瘤过程中起着重要作用.前期工作揭示vGPCR的氨基端Y26和Y28两位酪氨酸的巯基化作用对其致瘤性至关重要.本研究将vGPCR的氨基端(1～49位氨基酸)以及其yydd突变体分别同鼠Fc片段有机连接并命名为wt-vG-N...